...followed suit. Two Addgenies, Susanna Bachle and Yijun Zhang, recently attended virtual conferences (TAGC and...and ASGCT, respectively) and Addgenie Angela Abitua hosted a virtual Reproducibility for Everyone workshop...host will see the highly voted questions,” says Zhang. “Usually the moderator starts by asking the speaker... hosted in breakout rooms, or even on social media.Zhang was able to view posters without presenters “...to start. “There was not much time to network,” Zhang says. “The schedule was pretty busy, but after the... Jennifer Tsang...

...bioreporter spontaneously changes from green to orange such that non-growing cells are orange, slow growing cells... But the shape and metabolism of leaves can be changed by light and temperature and leaf autofluorescence...cells are orange-green, and fast growing cells are green. A second colonizer, labelled with a constitutively... Jennifer Tsang...

...meetup.com! Many thanks to our guest blogger Angela Kaczmarczyk! Angela Kaczmarczyk is a co-organizer at BosLab...2015, there were six of us. We were initially strangers but we all shared a passion for DIY Biology and...biohacking courses. Our members pursue a diverse range of projects at BosLab. Some are entrepreneurs using...RNA with small molecules. Other members’ projects range from hacking probiotics to filming educational lab... Angela Abitua...

...B978-012198382-6/50026-1 Wang R, Xiang S, Feng Y, Srinivas S, Zhang Y, Lin M, Wang S (2013) Engineering production...for specificity and sensitivity, for example by changing certain amino acids to improve its stability. ...single protein that can recognize the target protein (Wang et al., 2013). These proteins are also plasmid-derived.../10.1016/j.bios.2016.04.091 Cruse JM, Lewis RE, Wang H, Geziena MT, Steven GE, Lorna J. (2004) ANTIGENS...

... a broad pH range. The four biometric probes successfully targeted endosomes under a range of pH conditions...and RNA expression - but what if you just want to change protein levels? Until recently, no such tool existed...proliferation. These cells also did not contain changes in expression of TRIM21-associated genes, indicating...transcription factors in series and independently changes inducer levels. That is, if transcription factor...expression of a reporter protein, it is possible to change the amount of noise in reporter gene expression...

...trolls. People don’t like to connect with complete strangers as it borders on my first rule of networking--...a useful way to build or use a network. Total strangers can’t really help or refer you. Do not ask people... lot about requesting connections to complete strangers for informational interviews. Many have had success...game for connecting to others.  Pro tip: You can change the option for sending LinkedIn messages to Click...considered intrusive and it’s still connecting with a stranger so is highly discouraged. You should be using ...Turn off notifications to your network of profile changes. If you are working, and all of a sudden you start...

...resolve this issue, Shangqin Guo and her colleagues at Yale University fused a color-changing fluorescent timer...proteins system for Caenorhabditis elegans By Jennifer Tsang Fluorescent proteins (FPs) traditionally introduced...’s native locus, but introducing large genetic changes in C. elegans with CRISPR can be difficult since...synthesis and, after a few hours, a conformational change causes the protein to fluoresce red. H2B-FT-red...j.celrep.2020.107804 The CRISPR corner By Jennifer Tsang Here are a few highlights from recent CRISPR plasmids...

...of [after undergrad] was to learn and speak the language of science. I didn’t come out of [undergrad] feeling...work,” she said. “School will always be there.” Changing your path! It’s okay! Our blog writer, Beth Kenkel...research and technical assistant, when her life changed––she had a daughter. Needing a career switch because...thought, ‘Let me pursue this, let me make a slight change so I can have flexibility and be up to date with...Bioinformatics it is.’” Though she misses the bench, the change gave her the balance she needed. It is incredibly...incredibly helpful to me to know that you can make big changes in your career path and still find comfort and ...

...•T-to-G•C base changes (Figure 1), but it can be challenging to make these base changes, especially in... protospacer, the editing capacity of the ABE8s ranged from 1.5x higher for positions A5-A7 to 3.2x higher...hemoglobin. Previous studies have shown that one base change in the promoter region of gamma hemoglobin (HGB1...effects. ABEs have been described to introduce base changes in cellular DNA and RNA dependent or independent...authors tested ABE8.17-m and found that one base change mutation (ABE8.17-m+V106W) (Gaudelli et al., 2020...

...all incoming plasmids was a big change for Addgene - we had used Sanger sequencing for quality control ...fall within a 10 fold range. Plasmids that don't meet the lower bound of this range are “failed” and removed.... Priority number one was to ensure that this changeover had no impact on our ability to provide requesting...Abudayyeh OO, Platt RJ, Brigham MD, Sanjana NE, Zhang F (2017) Genome-scale CRISPR-Cas9 knockout and transcriptional...

...:10.1126/science.aas9129   Zhang W, Yin J, Zhang-Ding Z, Xin C, Liu M, Wang Y, Ai C, Hu J. In-depth assessment...patient mutation or need to make a precise amino acid change, there is very little flexibility in the location...shown to be less effective at NGH PAMs, though (Zhang et al, & Walton et al). Additionally, SpG Cas9 with...NGAG - xCas9 NG Less effective at NGH PAMs (Zhang et al, 2021) NG Cas9 NG - SpG Cas9  NG  ...

...there were many changes to the  Board of Directors and the Executive Director changed twice. I had to ...liaise with the Executive Director. As members changed, I had to learn how to develop new working relationships... had different styles from their predecessors. Changes were implemented and I had to adapt accordingly...write on behalf of the Advocacy Committee would change format depending on the particular style and requirements...growing too and with that would come necessary changes. I also learnt to adapt my leadership style according...

...O'Neill, P. S., Villette, V., Bradley, J., Wang, Z., Jiang, D., Zhang, G., … Lin, M. Z. (2024). A fast and ... J., Luebbers, A., Green, E., Philibert, C. E., Zhang, H., Layne, M. D., Wu, J. C., & Garcia-Marcos, M...diseases (Diabetes & cancer). Image adapted from Wu and Wang 2024 under a CC-BY 4.0 license. Find more neuroscience... neuroscience antibodies here! Wu, Z., Wang, W. (2024). Anti-APP [6E10-A5] in Immunohistochemistry (Human...

...CFD scores (which range between 0 and 100) are then converted to a weight (which ranges between 0 and 1)...These values (ranging from -40 bp to 40 bp) are also converted to a weight (which ranges between 0 and...cut-to-insert distance) This gives each guide a score ranging between 0 and 1. The cutting frequency determination...specificity of a guide to the target. The CFD score ranges between 0 and 100 for each guide, with 100 being...

... are sensitive to pH. Anything between orange and light orange usually indicates that the cells have grown... 37 °C and 5% CO2 and get agitated with sudden changes in temperature (especially if you have air conditioning... to recover after they are passaged. This could change their growth rates and morphology, rendering their...culture media contain the pH indicator phenol red (pH range 6.0 (yellow) - 8.0 (red)). Blood pH normally lies...numbers Keep an eye on the passage number. Cells change their growth rate and morphology at high passage...

...prepping. Be sure to stay within the recommended range of the filter or you might inadvertently lower the... the culture medium. Improve the culture media Changing to a richer media or adding supplements can improve... low copy plasmids or slower growing strains, a change in media may be advantageous. Reduce the antibiotic...for high copy plasmids in standard growth strains range from 12-16 hours, but cultures with lower copy plasmids...oxygen for optimal growth, as insufficient gas exchange will prevent your cultures from reaching the desired...shaker speed is fast enough for sufficient gas exchange in the overnight culture. Generally, large cultures...

...players in the CRISPR field. Just a year after Feng Zhang’s lab identified Cas13a (C2c2) (Abudayyeh et al.,...end joining (NHEJ). Designing an RNA editor The Zhang lab envisioned a two-component RNA editor: a Cas13...adenosine to inosine in RNA, creating a functional A->G change. They fused ADAR deaminase domains (ADARDD) to ...introducing a C opposite the target A to be edited. This change specifies the edit to be made when multiple As ...Capabilities through RESCUE Two years later, the Zhang lab expanded the RNA editing toolkit to allow C ... their RESCUE (RNA Editing for Specific C-to-U Exchange) system (Abudayyeh et al., 2019). To avoid some...

... dilution should fall within the range of the assay for a wide range of titers. We usually dilute our ...determine if your titers are within the expected range. We have also found that generating the standard...virus. It is important to note that the dynamic range of the ddPCR is between 1 and 100,000 genome copies...per reaction. Since AAV titers tend to be in the range of 1012 to 1013 GC/mL, you must serially dilute .../s is recommended to ensure an even temperature change among all of the droplets, but you can go as low...

...preferred buffer. If that’s the case, a buffer exchange can be performed using a desalting column or an...western blot. This can be due to conformational changes in the antigen or lower detection sensitivity of...antibodies may impact their quality and efficacy (Wang et. al., 2018). Aggregation can arise from a shift.... PMID: 23908655; PMCID: PMC3725456. Schwarz H, Zhang Y, Zhan C, Malm M, Field R, Turner R, Sellick C,...j.ijbiomac.2015.03.027. Epub 2015 Mar 25. PMID: 25819219. Wang W, Roberts CJ. Protein aggregation - Mechanisms,...A. Pathak, Christopher J. Roberts, Prasad S. Sarangapani, Protein aggregation, particle formation, characterization...

...olds in a single evening. Grades K-5 cover a broad range of attention spans, skill levels, and STEM exposure...Chemistry activities from Quatr.us Foldscopes Steve Spangler Science The Fab Lab Middle school: supplementing... key concepts (Fig. 2). One thing I will likely change for next time is giving fewer examples and think...regulated. I did not try to sugarcoat the potential dangers of people using the technology in an unethical ...aspects of talking to 8th graders was the broad range of questions I received. I gave my presentation ...each, and each group went on its own wonderful tangents. Some groups asked about vaccines, others were...