Technical Design of a Western Blot
Type
Blog Post
Published
Aug. 22, 2024, 1:15 p.m.
... incubation temperature for your specific protein(s) of interest.
Determining linear detection range
...assay or BCA assay. Be sure to aliquot your sample(s) before adding in your denaturing and/or loading buffers...that covers a size range appropriate for the target(s) and transfer method.
Membranes
The two membrane ...your standard curve, your standard, and your sample(s), as you can only compare samples to a standard curve...using a protein stain like Coomassie Blue or Ponceau S. It is far less prone to error than normalizing to...