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  1. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    Published
    Nov. 14, 2017, 2 p.m.
    ...make a career in academia, you are likely in good shape, as your own adviser will probably have a lot of...webpages. Importantly, find a good mentor that can share relevant experience and resources with you to ease...

  2. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ...at the limited number of hits. So, here, I will share my process with you. But before I do that, let me...formatting guidelines, etc.) you have some criteria to shape the document. Get and use a reference management...

  3. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    Published
    Oct. 13, 2016, 2:30 p.m.
    ...knowledge to a variety of problems. In this post, I’ll share what I’ve learned as a healthcare consultant and...as marketing drugs to physicians and patients, shaping discussions with insurance companies about the ...

  7. How to Be an Excellent Trainee

    Type
    Blog Post
    Published
    Sept. 26, 2023, 1:15 p.m.
    ...protocol steps and other tips and tricks your mentor shares. Additionally, go ahead and write down where that...that one drug is stored or how to access the shared server. The blue box labeled “A5” on the second rack...

  8. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...have a shape, like a box, ellipse, or circle, drawn around clusters to define populations. This shape is ...

  9. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ...frequency in a reference of highly expressed genes (Sharp and Li, 1987). In 2000 the Ikemura lab created the...Genet 12:683–691. https://doi.org/10.1038/nrg3051 Sharp PM, Li W-H (1987) The codon adaptation index-a measure...

  10. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    Published
    Jan. 4, 2022, 2:15 p.m.
    ...you are making your hypothesis stronger. When you share data at a conference poster session, you don’t necessarily...  Communication (or lack thereof): Information sharing gets more challenging the bigger an organization...

  11. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    Published
    March 28, 2024, 1:15 p.m.
    ...tissue morphogenesis, organ development, and the shaping of the extracellular matrix. During immune responses...cellular processes.   Figure 1: Integrins can shape into three conformations: (A) bent-closed, (B) extended-closed...

  12. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    Published
    Aug. 23, 2016, 2:30 p.m.
    ...the fine points of visual aids. Here’s a great slideshare with more details on visuals in science presentations...down it is a great segue to talk about how the sharing model works. See if you can elicit helpful comments...

  14. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    Published
    Nov. 7, 2014, 2:56 p.m.
    ... E. coli strains E. coli are gram-negative, rod shaped bacteria that were named after Dr. Theodor Escherich...strain is a bit more convoluted due to researchers sharing and renaming samples throughout history. It was...

  15. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...binding pocket of an engineered receptor that change shape with light exposure to activate them. PORTLs use... Chibukhchyan, A., Ramakrishnan, C., Inoue, M., Shane, J. C., McKnight, D. J., Yoshizawa, S., Kato, H....

  16. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ...optimizing!  Recommended Reading Issa, S. S., Shaimardanova, A. A., Solovyeva, V. V., & Rizvanov, A. A. ....org/10.1038/s41434-019-0075-6 Issa, S. S., Shaimardanova, A. A., Solovyeva, V. V., & Rizvanov, A. A. ...

  17. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    Published
    Jan. 16, 2025, 3:42 p.m.
    ...doi.org/10.1016/j.omtm.2022.07.010 Issa, S. S., Shaimardanova, A. A., Solovyeva, V. V., & Rizvanov, A. A. ...j.neuron.2016.09.021 Walters, R. W., Yi, S. M., Keshavjee, S., Brown, K. E., Welsh, M. J., Chiorini, J. ...

  18. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ...blocking for 1 h at room temperature with gentle shaking, gently wick off excess liquid from each membrane...start) and add your membranes to the solution, shaking gently for 1 h at room temperature. This will allow...

  19. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    Published
    March 6, 2018, 1:49 p.m.
    ...sequences, but are sensitive to distortions in helix shape (22). Large purine:purine mismatches and most smaller...hydrogen bonds and a base-pair size nearly indistinguishable from a Watson-Crick base pair, are joined ...

  20. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    Published
    Nov. 2, 2021, 1:15 p.m.
    ... model. For example, in a recent study by the Wickersham lab (Lavin, TK. 2020) it was determined that ...10.1089/hum.2019.277 Lavin TK, Jin L, Lea NE, Wickersham IR (2020) Monosynaptic Tracing Success Depends...

  22. Which Fluorescence Microscopy Techniques is Best for Me?

    Type
    Blog Post
    Published
    Oct. 10, 2017, 1:57 p.m.
    ... focus). This means that the images will appear sharp without interference from blurry out-of-focus light... lightsheet that projects a number of thin cone-shaped beams into the sample. These beams interfere to...

  23. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    Published
    July 11, 2017, 2:30 p.m.
    ...how science can be accelerated immensely when we share knowledge and reagents. I have not intentionally...PMCID: PMC4744125. 3. Sanjana, Neville E., Ophir Shalem, and Feng Zhang. "Improved vectors and genome-wide...

  24. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    Published
    April 25, 2017, 2:30 p.m.
    ...:19169259. PubMed Central PMCID:PMC2901231. 3.Verkhusha, Vladislav V., and Alexander Sorkin. "Conversion...25581799. PubMed Central PMCID:PMC4344411. 13.Berlin, Shai, et al. "Photoactivatable genetically encoded calcium...

  25. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ... also known as immunoglobulins, are ~150 kDa, Y-shaped proteins that are both a natural part of the immune... the Addgene blog: Read about another reagent sharing platform for antibodies, the Developmental Studies...

  27. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ...and reliable qPCR data, you’ll of course want to share it so others can provide you with the highest form....2018.12.002.Teo IA; Choi JW;  Morlese J; Taylor G; Shaun S. LightCycler qPCR optimisation for low copy number...

  29. New Western Blot Protocol Video!

    Type
    Blog Post
    Published
    March 28, 2023, 1:15 p.m.
    ... Addgenie Meghan and Abi run a Western blot and share tips for this tricky application.    You can check...

  30. Viral Vectors 101 eBook 2nd Edition

    Type
    Blog Post
    Published
    July 11, 2024, 1:15 p.m.
    ...We are very excited to share the latest edition of our Viral Vectors 101 eBook!  This practical guide...

  31. Antibodies 101 Series on TikTok

    Type
    Blog Post
    Published
    March 19, 2024, 1:15 p.m.
    ...isn't your platform, all of our videos will also be shared on our @addgene Instagram account, or you can watch...

  32. 2022 Depositor Week

    Type
    Blog Post
    Published
    Dec. 12, 2022, 2:15 p.m.
    ...revamped Data Hub! The community needs more data-sharing heroes like you.)  ...

  33. CRISPR Guide Update

    Type
    Blog Post
    Published
    Oct. 10, 2024, 1:15 p.m.
    ...With this update, we have added new sections to share and describe some incredible new CRISPR technologies...

  35. Congrats to the 2019 Deck The Lab Winners

    Type
    Blog Post
    Published
    Jan. 17, 2020, 3 p.m.
    ...submissions on Twitter and Instagram and we’re excited to share these creative entries as the winners of the 2019...

  36. Addgene at SfN23!

    Type
    Blog Post
    Published
    Nov. 9, 2023, 2:15 p.m.
    ... text, we invite you to join us! We'll also be sharing our conference experience on X, Instagram, and ...
Showing: 241 - 280 of 490 results