...amplicons.  Sybr has high background noise, due to the large amount of dsDNA in most starting materials, and ...

...Tips and tricks from the bench Don't pick too large of a colony. Too many bacteria can inhibit your ...the insert site. A positive clone will produce a larger size product then a negative clone without the ...-) will not. Backbone-specific primers (2) give larger sized products for positive clones (+) compared...

.... Picking receptors The receptor you choose is largely dependent on what cellular response you aim to ...

... prone to recombination, while a library with a large backbone might be more difficult to transform.  ...generally needs to be determined experimentally. For larger libraries that require hundreds of nanograms of...full-size plasmids (or for smaller plasmids to overtake larger plasmids, if the library varies significantly in...

...proteasome for degradation. The HaloTag is quite large (33 kDa) and does not self-cleave off of the protein...

... This specific example is attractive due to the large number of sheer molecules and signal you can generate...

...groups or, by extension, race. Many researchers do large-scale CRISPR screens in cancer cell lines, looking...address this issue, but it still points to a far larger and more complex problem endemic to biomedical ...

...Permeabilization What is the point?  Antibodies are large proteins that need a little help crossing cell membranes...

... parts. That was the motivation for releasing a large parts kit. Once you have so many parts available...

... called Slow-FT, Medium-FT, Fast-FT, and mK-GO. Large Stokes Shift (LSS): Stokes shift (named after George...discovery, the family of FPs just keeps getting larger with hundreds of variants available. Read on to...

...commonly used to create functional knockouts or large deletions, the ability to analyze and interpret ...

... are just 2 examples of organizations that hold large, annual conferences. Gordon conferences offer more... Career Expo and the ACS career fair, which are larger meetings offering resources for students seeking...

...experience. With this valuable training behind you (and largely only that is necessary), there are opportunities...to manipulate cells (manufactured by one of the larger biotech companies), and as far as I remember, it...

...from purified recombinant proteins at small and large scale.  This new technique involves selection in...

...using RNA-seq. Since the REPAIRv1 machinery is too large to fit into adeno-associated viral vectors, they...

... Design and selection for targets can be done largely in vitro; Easy and reproducible commercial synthesis...metal ion, or fold to incorporate themselves into a larger target, such as a protein. Such flexibility makes...

..., to search the literature, and to synthesize a large volume of information: basically, a scientist’s ...

...Gibson assembly method Single reaction, can assemble large DNA sequences, no restriction enzymes needed Not...

...Addgene’s ready-made entry clones can be used with a large variety of plasmids. Speed The Gateway system enables...

...diagnosis indicate, for Stage 3, that the cancer is large in size and may have spread while Stage 4 indicates...

...Zebrafish mutants In the 1990’s, two revolutionary large-scale random mutagenesis screens produced ~1,500...

...insertions and deletions (indels) most likely to have large phenotypic effects such as those that affected gene...

...effectively a million dollar sales pitch. Given this large sum, one might expect that applicants would work...

...about the advantages of pooled CRISPR libraries for large-scale functional screens Read this blog post about...

... purification procedures. Even if you produce a large amount of protein in your expression host of choice...

...proteins are split between strand 10 and 11 to yield large (1-10) and small (11) fragments. In several cases...

... and structure. Their porous surface provides a large area for binding, but antibodies conjugated within...

...vectors are replication-deficient. Thus, a very large number of phagemids would be needed for an in vivo...

...variants use different RT enzymes and performed large edits with similar or better efficiency than PEmax...

...properties of the final product, particularly in large animal studies. Endotoxin contamination is minimized...

...outlined below, allow for researchers to select large sets (with overhangs that violate some of the traditional...

...Interestingly, he also adds to the disproportionately large number of honorees to have trained under a previous...fortunate enough to apprentice with top scientists to larger groups of trainees at an earlier point in their...

... from degradation. When designing epegRNAs with large structured motifs like mpknot, use the pegRNA Linker...

...pipetting small volumes (< 2 µL) or volumes too large for a standard micropipette (> 1,000 µL). The standard...

...editor experimentalist, and especially shines once large-scale prime editing experiments come into play. ...

... did, and state that it would help you manage a large number of files as part of the job. Structure of...

...software was originally designed to help us work in large formats, such as 96-well microplates, and thanks...realized the need to modernize our approach to handle larger volumes of data. In this two-part blog series, ...

... vivo (Slimko and Lester, 2003).  Addgene has a large selection of codon optimized genes, so check out...

...information in a small space and is ideal for tracking large collections. Many companies, such as Thermo Fisher...

...been very difficult for us to respond to such a large number of requests by ourselves without Addgene....