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Showing: 181 - 210 of 459 results

  2. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    Published
    Dec. 12, 2019, 2:22 p.m.
    ...visible part behind these changes: the backend technology that powers the new website search. Tackling ... resources, blog posts or reagent items.  The technology behind Addgene’s new search  Like millions of...

  4. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    Published
    May 9, 2017, 2:12 p.m.
    ... is one of the latest developments in CRISPR technologies.  It’s a simple and effective method with multiple...CRISPR–Cpf1 Asing a Single crRNA Array." Nature Biotechnology 35.1 (2016): 31-34. Pubmed PMID: 27918548. 2...

  5. Hot Plasmids - May 2022

    Type
    Blog Post
    Published
    May 26, 2022, 1:15 p.m.
    ... for example).  Given that the liver is an immunologically active organ, this advance is likely to afford...Kaufman, et al, 2022.    This new barcoding technology would allow researchers to avoid the off-target...

  6. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    Published
    April 5, 2022, 1:20 p.m.
    ...multiprotein complexes.  Fig. 2. Multiplex immunolabeling with subclass-switched recombinant antibodies...monoclonal antibodies for enhanced multiplex immunolabeling of brain. Elife. 2019 Jan 22;8:e43322. doi:...

  7. Hot Plasmids - February 2022

    Type
    Blog Post
    Published
    Feb. 22, 2022, 2:15 p.m.
    ...live-cell imaging, single-molecule tracking, immunolabeling of fixed cells, Western blotting, and more....future genetic screens.   DeWeirdt PC, et al. Nat Biotechnol. 2021. doi: 10.1038/s41587-020-0600-6. The Penn...

  8. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    Published
    June 15, 2023, 1:15 p.m.
    ...allow a protein to be more effectively used in a technology or as a therapeutic.  A (very) brief history ... mostly accessible to experts. That many GCE technologies have idiosyncrasies presents a significant barrier...

  9. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    Published
    June 6, 2023, 1:15 p.m.
    ...Schambach A. Gammaretroviral vectors: biology, technology and application. Viruses. 2011 Jun;3(6):677-713...An Overview of the Application of Viruses to Biotechnology. Viruses. 2021 Oct 14;13(10):2073. DOI: 10.3390...

  10. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    Published
    June 2, 2015, 6:06 p.m.
    ...you have any questions or thoughts about this technology, leave them in the comments below.  References... Reyon D, Goodwin MJ, Aryee MJ, Joung JK. Nat Biotechnol. 2014 Jun;32(6):569-76. doi: 10.1038/nbt.2908...

  12. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    Published
    Nov. 21, 2019, 1:53 p.m.
    ... the lab to be using words for their field or technology in a consistent way. Just creating a lexicon ...first weeks or months when I was learning the terminology of a new field. Annotate: When creating important...

  13. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    Published
    Oct. 29, 2019, 2:52 p.m.
    ...Tools for studying bioelectricity Dyes and other technologies are now available for fluorescent reporting ...synthetic bioengineering." Current opinion in biotechnology 52 (2018): 134-144. PubMed PMID: 29684787. Oviedo...

  14. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    ...Colorful CRISPR technologies are helping researchers visualize the genome and its organization within...and engineered sgRNAs using CRISPRainbow.” Nat Biotechnol. 34(5) (2016): 528-30. PubMed PMID: 27088723....

  15. Developing Lab Management Software for Biology

    Type
    Blog Post
    Published
    Aug. 25, 2016, 2:30 p.m.
    ...about how they would like these things tracked. Terminology and language are tricky Names for things will...running and more time working on interesting biotechnology code.There are valid technical reasons to choose...

  17. To Each HIS Own

    Type
    Blog Post
    Published
    March 2, 2023, 2:15 p.m.
    ...Proteins with a Novel Metal Chelate Adsorbent. Bio/Technology. 1988; 6: 1321–1325. Janknecht R, de Martynoff...growth factor I from culture medium of E.coli. Bio/Technology. 1987, 5:379-382. Bornhorst JA and Falke JJ. ...

  18. Antibodies 101: Conjugation

    Type
    Blog Post
    Published
    March 26, 2024, 1:15 p.m.
    ...References and resources References Bendall SC, Nolan GP, Roederer M, Chattopadhyay PK (2012) A deep profiler... profiler’s guide to cytometry. Trends Immunol 33:323–332. https://doi.org/10.1016/j.it.2012.02.010 Dennler...

  19. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    Published
    July 11, 2019, 1:26 p.m.
    ...first blog post in this series, I’ll highlight terminology you should be familiar with before working with...Cre is expressed.  Several variations of this technology can be used to customize the effects of this ...

  20. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    Published
    July 23, 2020, 1:20 p.m.
    ...updated Jul 23, 2020 by Jennifer Tsang. CRISPR-Cas technology is constantly evolving. Variants of Cas proteins... applications: natural brakes for CRISPR-Cas technologies. Nat Methods 17:471–479 . https://doi.org/10.1038...

  22. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    Published
    March 8, 2017, 3:15 p.m.
    ...team will communicate with your organization’s technology transfer office or other authorized office directly... ship the samples to Addgene and contact your technology transfer office about the MTA. Pro Tip! Download...

  23. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ...network of your peers. Furthermore, using new technologies to solve a problem can allow you to approach...other scientists that we forget that some of the terminology we use routinely isn’t common to non-scientists...

  24. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    Published
    July 14, 2016, 2:30 p.m.
    ...specificities of Cpf1 endonucleases in human cells. Nat Biotechnol. 2016 Jun 6. PubMed PMID: 27272384 Find plasmids...-Cas Cpf1 nucleases in human cells." Nature Biotechnology (2016). PubMed PMID: 27347757. Find plasmids...

  25. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    Published
    Dec. 1, 2015, 3:30 p.m.
    ...Fengshan Forest Park. As a national science and technology park, it enjoys preferential governmental policies...high-tech development zone so many science and technology companies and start-ups choose to locate there...

  26. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    Published
    April 6, 2021, 12:30 p.m.
    ...Aug 3, 2017 and last updated Apr 6, 2021 by Will Arnold. Introducing NGS in 2017 for all incoming plasmids...Partnering with seqWell, we are using the plexWell technology to easily and quickly create Illumina sequencing...

  27. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    Published
    June 22, 2023, 1:15 p.m.
    ...bleach (plastic) or ethanol (other surfaces). For AAVs, which are resistant to ethanol and isopropyl alcohol...

  28. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    Published
    Aug. 1, 2024, 1:15 p.m.
    ... most PVDF membranes recommend re-wetting with methanol before rinsing with wash buffer, as dry PVDF won...Buffer SDS 2% (w/v) Detergent Β-mercaptoethanol (βME) 100–115 mM = 0.7%–0.8% (v/v) Breaks...

  29. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    Published
    Sept. 26, 2019, 12:50 p.m.
    ...efficiency and off-target effects compare to existing technologies, and their use for HDR editing and mouse-model...compared head-to-head with existing CRISPR delivery technologies, Nanoblades had higher rates for editing efficiency...

  30. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ...control protein expression for experimental, biotechnological, and medical applications. Researches can ...desired protein. Despite being a rather nascent technology, plant cells have been used to express a wide...
Showing: 181 - 210 of 459 results