... or set of plasmids. We hope that these articles make it easier for you to find and use the plasmids you...for in vivo expression, and/or produce vectors to make mutant cell lines or transgenic animals.   Wall...To expand the capabilities of Gibson assembly and make a more modular platform, the Jacks lab generated...cumbersome to use in a high throughput manner or make it difficult to quantify interaction strengths. ...Cas9-based AID tagging Seven years ago, Masato Kanemaki's lab successfully used the plant-based auxin-inducible...introduction of auxin range from 10-20 minutes, making AID an appealing tool for studying essential proteins...described in their recent Cell Reports paper, the Kanemaki lab overcame this hurdle using CRISPR technology...

...smallest bacteria. The absence of a rigid cell wall makes them resistant to antibiotics and antibacterial ...interpretation. Also, the signals from the nuclear area make the detection of mycoplasmas difficult (Ligasová...contamination when you are working without gloves or masks. Make sure to change the gloves and masks regularly instead...line stock for mycoplasma contamination before use. Make sure there is no contamination present in the serum...disposable pipettes to avoid cross-contamination. Make sure you keep all the required equipment and reagents...Avoid long exposure of the cell culture to air and make sure to tighten the flask cap before placing them...

... an incredibly fast-moving field, and we want to make it easy for you to keep up with new developments...to update our web resources and plasmid pages to make them more useful and easy to navigate. We also updated.... Base Edit: Base editing plasmids allow you to make precise C:G->T:A or A:T->G:C edits in DNA without...Like RNA targeting systems, RNA editing does not make changes to the genome, thus providing an avenue ...CRISPR Genome Editing and The CRISPR Software Matchmaker Day 2: Homology directed repair: CRISPR 101: ...

... and then condensing that and making it accessible to students.” Making science approachable is often ...eScience Labs.  “We work with faculty members to make sure the courses encompass what they want to teach...onsite, Caitlin can visit the operations team to make sure “that everything that’s going into the kit ...non-profit Fred Hutchinson Cancer Research Center. And make sure to subscribe to the Career Channel of the blog...

...this responsibility seriously. Every decision we make, every project we undertake and every service we... that our materials are affordable but enough to make us self-sustaining. With this unique model, we are...opposed to fundraising.   Aside from enabling us to make all decisions with the goal of  benefiting our scientific...ever curious what specifically drives the decision making process at Addgene, just look to our core values...curated, high-quality data and reagents Addgene makes it easy for scientists to deposit and request plasmids...

... science brick-by-brick”. Our mission is to help make science mainstream and to show people the very human...at these struggles will bring people together and make these daily challenges a little easier to overcome... As we started building, we thought – how can we make the connection between LEGOs and science more concrete...and sounds to synchronize with the images thereby making the videos more real. To give you an idea of the...amount of work, see how many pictures we take to make a walk cycle of one step. Challenges in the LEGO...

... programs and it seems to me that most Addgenies make an effort to avoid plastic bags and to conserve ...conservation in the lab One stingy science project was to make my own glass milk for purifying cut plasmid DNA ...amount of wasteful packaging.  Turns out, one could make a lifetime supply for about $10 US dollars worth...accessed blogs on BitesizeBio is the protocol for making your own Taq polymerase by Outreach Scientist, ...founder, Melina Fan, recently shared our protocol for making competent cells to save time and money. My other...

...Resource Identifiers (RRIDs) for each antibody, making it easy for you to determine whether any given ...been, a community resource that supports both toolmakers and tool users. We encourage you to share your...resource. We are excited to work with all of you to make this the best and most accessible recombinant antibody... are some ways that you can participate: If you make recombinant antibodies or other recombinant affinity...get started.  If you have suggestions about how to make the resource better or ideas for new materials to...

...DNA, but they still rely on the host machinery to make additional copies. ori sequences are generally high...replication machinery room to come in and get busy making copies.     Types of origins of replication ...plasmid type in your cells at a time? Do you want to make a lot of your plasmid? Is the gene toxic in high..., so if you need two plasmids for an experiment, make sure they have "compatible" ori's. See the table...more copies with the addition of chloramphenicol – make sure your growth conditions aren't working against...

...Gearing explains how Cas9 homolog, Cpf1, may make it easier to make gene edits at new genomic locations. 6.... Why and Where Do I Find One? If you need help making a transition into a new job or maybe to an entirely...gene delivery, but have limited packaging capacity making them difficult to use with CRISPR as the cargo....Your Genome Edit Once you’ve used CRISPR/Cas9 to make a change to the genome, you need to check whether...

...within the UV spectrum, while wavelengths over 700 nm make up the infrared (IR) spectrum. When photons are ...advantage of a brighter fluorophore? Strong brightness makes it easier to distinguish a signal from noise; therefore... together in the same color panel. In this case, make sure to compare the excitation/emission spectra ...www.bdbiosciences.com/en-us/resources/bd-spectrum-viewer.   To make life easier, always try to use fluorophores with...worry, there are strategies to resolve this issue. Make sure to check out our blog post on color compensation...and 2) BV421 is a very bright fluorophore, which makes it easier to detect the transgenic protein. We don't...

...At Addgene, it is our mission to make it easy for you to share plasmids. To achieve this goal, we will...and perform standard quality control but will not make the plasmids available until you update us with ...by sequencing. Upload any support files that will make it easier for scientists to best utilize your plasmids...it appears in the Entrez Gene list, as this will make it easier for other scientists to find information...and Mammalian Expression). This information will make it easier for scientists to find your plasmid by...Please review all of the information on this page to make sure it is correct. You can easily update the information...

...action. Instead of rushing in, you take steps to make it more likely you’ll get your tasks done correctly...to employers because they save time and money and make employees more productive. Careers where you’ll ...applicants compared to the number of available roles). Make your resume stand out Before you begin to apply ... need to think of other aspects of yourself that make you stand out. For every role you need to show that...Grad Student to Entrepreneur Learn to Network and Make the Contacts for Your Dream Job Resources on Addgene.org...

...the readability of your writing. It makes suggestions on how to make your writing less complex. While I...’s a list of tools and resources that might help make writing a little easier. Writing schedule tools ... should accept all of the suggestions Hemmngway makes (it wasn’t designed for technical writing), it’s...and you can share your library with others which makes it easier to work with collaborators. Zotero is ...

...well-understood standardized terms makes it a lot easier for organizations to make policy decisions on research...positive feedback on “our” MTAs, which sometimes makes people think our MTAs are different than other institutes.... Setting clear expectations from the beginning makes the sharing process smoother, more consistent, and...Stopwatch award and seek feedback as to how we can make our process easier for others.  If you’ve still ...

...working toward a solution. At the end of the meeting, make sure to thank everyone for their participation and...through a meeting with no idea why you are there. Make the most of everyone’s time by considering the level...grows, so will the length of your group meetings. Make sure to keep them manageable. At Addgene, teams ...last meeting. Use some positive peer pressure to make sure everyone completes their assigned items to ...like to send around a Google doc where people can make comments. At meetings, we just focus on the parts...

...documentation helps make sure everyone is on the same page. Effective delegation allows you to make the best use...expected to DO all the work – she is expected to make sure the work gets done and done well. Involving...for failure by delegating is a bad idea. How do I make sure it goes well? As with most managerial responsibilities...mistakes will be made that could have been prevented.  Make sure everyone understands that no question is “dumb...

...goals. You must understand your own style before making this choice.  How big is the lab? While larger ... the position. Am I choosing the right mentor? Make sure your advisors will be good role models and ...non-academic career path?  If you are considering this, make sure to choose an advisor that supports both paths...is one case where the topic of your research can make your transition easier – choose a lab that works...always be in the zone, but even brief stays there can make all the difference. Choose a lab that brings on ...

...Gel issues usually come down to voltage and time. Make sure to load an appropriate protein ladder on your...issue and can’t figure out what’s causing it, try making fresh running buffer. Old or improperly made running...in our Technical Design of a Western Blot post to make sure you have the optimal gel for your experiment...transfer. If you are expecting low amounts of protein, make sure your reversible staining method of choice is... fairly quickly, even at 4 °C, so when in doubt, make up a fresh batch.  Antibodies We always recommend...the solution used to expose them. You’ll need to make sure the sensitivity of your exposure solution matches... amounts directly, stripping and reprobing. Just make sure to go from least to most abundant protein if...

...the improvements we’ve implemented and how they’ll make it easier for you to find the plasmids you need....SnapGene powered plasmid maps at Addgene  Three factors make our SnapGene powered plasmid maps far easier to ...identities of most features labeled directly on them making them much easier to quickly identify. Improved ... to paste directly into your notebook. This will make it easier for you to share plasmid information with...their functions. We hope that these updates will make it much easier to browse the repository and quickly...