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We narrowed to 181 results for: grna

Showing: 101 - 120 of 181 results

  1. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...subsequently tested the original BE3 and HF-BE3 using a gRNA with high off-target cutting, and they found that... constructs (wild-type and truncation mutants), gRNAs used for TZAP gene editing, and a Hi6xs-MBP tagged...as Cys4, tRNA, and ribozyme systems for multiplex gRNA expression. Expression of toolkit components can...compatible backbones that can be combined with your gRNA and repair templates of choice though modular cloning... a single, unique, and constitutively expressed sgRNA targeting the 5’ end of each essential gene. Koo...

  2. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ... system uses light to deactivate photocleavable gRNAs within seconds. A CRISPR-based lineage tracing tool...cerevisiae genes with 6-12 guides per gene where gRNA expression is induced by anhydrous tetracycline....

  4. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ...Then choose which Cas enzyme to use and design a gRNA to where you would like to introduce the edit. Finally....org Browse All CRISPR Plasmids Find Validated gRNAs for Your Next Experiment Check out our CRISPR Guide... (Yu et al., Nat Chem Biol). Mutate the PAM or sgRNA sequences Cas9 likely goes through multiple rounds...

  5. Design Tips for Prime Editing

    Type
    Blog Post
    Published
    Jan. 23, 2025, 2:15 p.m.
    ... a prime editing guide RNA (pegRNA). The pegRNA consists of a CRISPR gRNA extended to include a primer...extension of the pegRNA should not be C. A C base is speculated to base pair with G81 of the gRNA (Anzalone ... the end of the pegRNA. Adding 3′ polyU tracts to the end of pegRNAs (but not epegRNAs) can improve either...left), while pegRNAs that use other bases in this location bind to Cas9 with canonical sgRNA structure (...your tool of choice. Nicking sgRNAs PE3 and PE5: These tools use an sgRNA to guide nicking of the unedited... editors bound to pegRNAs drive editing (left), while prime editors bound to sgRNAs only drive nicking...Created with BioRender.com. RNA protectors epegRNAs: epegRNAs include structured motifs at their 3′ end...

  6. Addgene’s Top 10 Blog Posts from 2014

    Type
    Blog Post
    Published
    Dec. 16, 2014, 8:01 p.m.
    ...Most Popular Posts of 2014 How to Design Your gRNA for CRISPR Genome Editing By John Doench (Guest...

  7. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...1143-8.  A new tool for CRISPR gRNA validation Selecting a gRNA sequence that effectively targets...DNA break. Next, the gRNA being tested is expressed along with Cas9. If the gRNA effectively cuts the ...for scientists to validate the efficacy of their gRNAs. The first step is to clone the target sequence ...genome-wide knockout screens. These vectors and sgRNA libraries expand upon the CRISPR family of plasmids...plasmids by offering a lentivirus-based mechanism for sgRNA delivery and providing a means for large scale functional...

  9. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    Published
    March 27, 2018, 1:32 p.m.
    ...use a dual vector system composed of SaCas9 and a gRNA in one AAV and a repair template in second a second...small Cas9 homologs like SaCas9 and their requisite gRNAs take up essentially all of an AAV’s ~4.5 kB packaging...

  10. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    Published
    Aug. 20, 2020, 2:30 p.m.
    ...check that your tracrRNA and crRNA (or synthetic gRNA) are derived from the same species. Expanding the...Guide Browse All CRISPR Plasmids Find Validated gRNAs ...

  12. Editor's Choice, August 2016

    Type
    Blog Post
    Published
    Sept. 2, 2016, 1:32 p.m.
    ...the transitions at target sequences specified by gRNAs. Caveats, potential specificity issues, and other...

  13. What's New in CRISPR - September 2019

    Type
    Blog Post
    Published
    Sept. 10, 2019, 12:56 p.m.
    ...lab. The Bikard library contains ~93,000 unique gRNAs that target random positions along the E. coli MG1655...targeting requires two sgRNAS: one that targets the knock-in site and a universal sgRNA that targets the donor... the genomic loci of interest. As the universal sgRNA is not predicted to target sites in the human, pig...

  14. Build Your CRISPR Vocabulary

    Type
    Blog Post
    Published
    July 2, 2024, 1:15 p.m.
    ...spacer and the remaining sequence as the scaffold. gRNA: "Guide RNA" is a general term referring to the ...require a tracrRNA. sgRNA: “Single-guide RNAs” were invented by synthetic biologists. An sgRNA combines a crRNA... tracrRNA pair or an sgRNA. It is often used interchangeably with the term sgRNA. Seed sequence: The 8...complementary DNA sequences. In laboratory settings, an sgRNA includes the crRNA and tracrRNA sequences in a “... on the 3’ end of an RNA guide (either crRNA or sgRNA). Cas9 requires perfect complementarity in this ...
Showing: 101 - 120 of 181 results