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Addgene
Showing: 101 - 120 of 224 results

  1. Top 10 Open Science Developments of 2013

    Type
    Blog Post
    Published
    Dec. 19, 2013, 3:46 p.m.
    ...published systematic reviews on an agent used to promote bone growth based on data voluntarily made available ...

  3. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    Published
    Aug. 4, 2015, 2:30 p.m.
    ...for microscopy enthusiasts! Addgene has empty backbones containing GFP variants used by Sattarzadeh et...

  6. SunTag and Fluorescent Imaging

    Type
    Blog Post
    Published
    March 28, 2017, 2:30 p.m.
    ...on Addgene.org Find Fluorescent Protein Empy Backbones Visit Our Fluorescent Protein Guide Page Find ...

  7. Antibodies 101: Affinity Tags

    Type
    Blog Post
    Published
    Sept. 19, 2023, 1 p.m.
    ...six consecutive His residues (or more). Histidine bonds with immobilized metal ions (Nickel, Cobalt, Copper...

  9. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    Published
    March 13, 2014, 4:08 p.m.
    ...designing oligos for cloning my target sequence into a backbone that uses the human U6 promoter to drive expression...

  12. Antibodies 101: Epitope Availability

    Type
    Blog Post
    Published
    Jan. 11, 2022, 2 p.m.
    ...using heat and/or chemicals to break some of the bonds keeping the protein folded, changing it from its...

  18. Plasmids 101: Origin of Replication

    Type
    Blog Post
    Published
    Nov. 11, 2020, 2:23 p.m.
    ... base pairs are held together with two hydrogen bonds not three as G-C pairs are. As a result, stretches...

  19. Plasmid Cloning by PCR

    Type
    Blog Post
    Published
    March 29, 2016, 2:30 p.m.
    ...MCS)), but do not cut elsewhere on the plasmid Bonus: It is helpful to choose restriction enzymes that...
Showing: 101 - 120 of 224 results