... are you’ve used a GFP derivative to conduct subcellular localization studies or make a reporter construct...et al. noticed that GFP had turned red. They subsequently confirmed that purified GFP could be imaged ...Find plasmids from this publication at Addgene. Subach OM, Patterson GH, Ting LM, Wang Y, Condeelis JS...

...to silence or incite neuronal firing and study subsequent effects on behavior. The system is an especially...the lab expressed the LiGluR opsin in different subsets of spinal cord neurons using the Gal4/UAS system...blog posts Read blog posts about viral vectors Subscribe to the Addgene blog Resources on Addgene.org ...

...NanoLuc, require injection of substrate to produce bioluminescence. Substrate pharmacokinetics and biodistribution...gastrointestinal tract. Require Administration of Substrate: Some luciferase reporters, such as Renilla luciferase...

...with smaller nick distances. Using D10A, they subsequently showed that editing efficiency is very low when...Cas9 with the left gRNA across the window. In a subsequent experiment, they were also able to introduce ...and Yan next examined a situation thought to be suboptimal for HDR. At the AAVS1 locus, the two nearest ...

...targeted to specific tissues, cell types or even subcellular regions of a neuron. Optogenetic approaches excel... excel at discrete cell or tissue-subregion targeting down to the limits of light delivery. Chemogenetic...DR If precise spatiotemporal control of a small subset of neurons is necessary, use optogenetics. If broad...

... tested the ability of GeoCas9 RNPs to cleave substrate when the reaction was carried out at elevated ... in diluted human plasma for 8 hours at 37°C. Substrate was then added and enzyme activity measured. SpCas9...reaction buffer for 8 hours at either 37 °C or 4 °C. Substrate was then added and cleavage product analyzed on...

...total of eight evolutionarily conserved CRISPR subtypes comprised of more than 40 cas gene families have...grouped the systems into three distinct types and subtypes using polythetic classification based on evolutionary...system and are not translatable to the other CRISPR subtypes. Still, the large and dynamic range of CRISPR ...

...labs. This week we are pleased to announce the submission of the results of our first study which can be...PubMed PMID: 16528756. 2. Oh, Steve KW, et al. "Substantial overproduction of antibodies by applying osmotic...Amin Hajitou. "Clathrin-mediated endocytosis and subsequent endo-lysosomal trafficking of adeno-associated...

...interest in STEM subjects; we generally refer to them as the “interested public.” Subscribers to Scientific...Common ones include age range, familiarity with the subject, shared interests, socioeconomic and/or racial/...

... (Fig. 1). However, once thawed, there was a substantial reduction (from 7.4 to 5.0) in the pH of samples...periods, and measured the pH. We found that tubes subjected to a recovery period of at least 96 hours had ...infectivity of the virus. However, there was a substantial reduction in infectivity after 3 weeks of exposure...

...temperature ranges and make them grow better at suboptimal ones, which could allow even your regular E. ...worth the trouble.  Enter ELSA: E. coli Learning Suboptimal Acclimatisation To resolve this impasse, our ...mesophilic chaperones would not function well under suboptimal conditions. This means recombinant proteins can...

...recombineering to create insertions, deletions, and substitutions - at the same efficiency as Gibson Assembly ...amplicons based on sequence homology, and the subsequent product is then digested and ligated into an ...nick repair is needed. Since the bacteria are subsequently grown at 37 °C, the recombineering plasmid is...

...ii) embedded in a gel, or (iii) attached to a substrate. These in vitro methods allow for a well-controlled... exchanging excitation filters). This avoids substantial changes in excitation power and will help to ...Backbones with Fluorescent Tags Find Plasmids for Subcellular Localization Browse the entire Fluorescent Protein...

...Nanobodies binding GFP were designed in 2009. Subsequent work showed that nanobodies could be fused to...suddenly seemed very realistic. Envisioning GFP as a substrate promoting dimerization, Tang et al. tested pairs...soon to allow genome modification only in a small subset of cells? Other proteins may also be utliized as...

...cell. Once this first order infection occurs, subsequent cycles of viral replication and retrograde transmission...viral replication are toxic. Second, infecting subsequently higher and higher order neurons doesn’t reveal...but the newly released RABV cannot infect any subsequent cells due to lack of G protein on the viral envelope...

...recombinant form through DNA sequencing of the line and subsequent cloning of the antibody chains (Crosnier et al... mammalian cells, for antibody production and subsequent purification.  Academic laboratories and companies...Engebrecht J, Trimmer JS (2019) A toolbox of IgG subclass-switched recombinant monoclonal antibodies for...

...Every few months we highlight a subset of the new plasmids and viral preps in the repository through ... were exposed to blue light. Cre-Vvd showed substantially improved activity as compared to other recombinase...fluorescent reporter. Find the plasmids here Yongsub Kim’s lab engineered prime editors to expand their...

...unusual substrates that other organisms can’t use. Other plasmids offer resistance to toxic substances like...possess plasmid-borne toxins like colicins and subtilisins to kill other bacteria in the area, freeing up...

...expensive exogenously-added substrate, chromoproteins don’t require other substrates for screening. Thus chromoproteins...distinction by name, chromoproteins are actually a subset of the fluorescent protein family. What sets them...

...extracted from purified, DNase-treated AAV and submitted for NGS. Raw sequencing data is analyzed to determine...sequences. Recombinase-dependent sequences may be subject to recombinase-independent recombination during...Check out our Viral Vectors Featured Topic Page Subscribe to Viral Vectors Blog Posts Learn How We Use NGS...