...chose needed to fulfill certain requirements: Customizable to our specific needs Modern and well-supported...the order and usage of the scripts needs to be customized in the pipeline for the specific needs of the...Since each process can use a unique container, we customized each container for its particular purpose and...bbduk process.    At first, to make sure we understood the concept of channels and implemented the input...user interface. In our next post, we’ll share our story about how we used Nextflow Tower to augment our ...

...Stochastic multicolor labeling is a popular technique in neuroscience and developmental biology. This... persistently fluorescent during development. Stochastic multicolor labeling systems, many based on Brainbow...for improving the level of detail possible for stochastic multicolor labeling of the brain. Labeling Drosophila...express the same XFP transgene in an adult fly. As a stop cassette is located directly upstream of the fluorescent... researchers who developed Tetbow. Conclusion Stochastic multicolor cell labeling systems allow single...

...situ hybridization histochemistry: use for mRNA detection.” The Journal of Histochemistry and Cytochemistry...interaction has also been adapted for use in immunohistochemistry and antibody labelling. In these experiments...referred to as the Avidin-Biotin Complex (ABC) immunohistochemistry (IHC). For more information on ABC and IHC...Cytochemistry : Official Journal of the Histochemistry Society, 41.12 (1993): 1751–1754.PubMed PMID: 8245422. Additional...

...proteins from E. coli, Bacillus subtilis, or Pichia pastoris depending upon your specific protein folding needs...alleles in microbial populations through time, Christopher Marx's lab has engineered and validated a new...one-pot reaction. Using this system, you can create custom RNA binding proteins in as little as 3 days. Abil...Abil et al., 2014 show that you can fuse custom PUF proteins to a translational repressor to down-regulate...Rajarshi, et al. "Engineering RNA endonucleases with customized sequence specificities." Nature communications...

...papers or are brainstorming new technological innovations. Let’s take a look at three historical techniques...that save both time and money. Yet, knowledge of historical techniques comes in handy whether you are perusing...throughout the world. Let us know your favorite "Historical Lab Technique" in the comments section below!...Benjamin Chain. "The sequence of sequencers: The history of sequencing DNA." Genomics (2015). PubMed PMID...

...Science History Rap Battle A great example of science story telling following the unfortunate story of Rosalind...and their importance in understanding biological history. 4. Please Don't Kill My Hive Beautifully filmed...

...Location, Location, Location! Failure to Plan (for Storage) Is Planning to Fail Patience Is Bitter but Its... the transport issues. 2. Failure to plan (for storage) Is planning to fail Freeze/Thaw Cycles Of course...cycles can still reduce AAV titer. For long term storage, make > 25 μl aliquots in 0.5 ml tubes, snap freeze... liquid nitrogen or a dry ice/ethanol bath, and store at -80° C. Once thawed, the AAV aliquot should be... PubMed Central PMCID: PMC3098627. 6. Salegio, Ernesto A., et al. "Axonal transport of adeno-associated...

...documenting your plasmids, storing them, providing quality control and customer support, making plasmids...Yesterday we hit a huge milestone here at Addgene – 500,000 plasmids shipped! That's a 1/2 million plasmids...The trust of our depositors to allow us to help store and distribute their plasmids. The partnerships ...

...equilibrium” where the cancer stops growing, and the immune system stops fighting. Such quiescent cancer...study cancer from every possible angle to leave no stone unturned, but cancer has been producing landslide...landslide after landslide, only adding more stones to the field. Fortunately, our bodies have been fighting ... downregulate the expression of the major histocompatibility complex (MHC) class I proteins on their surfaces...

...the CRISPR types and is thought to be the common ancestor of all other CRISPR systems. Type III systems ...(like me!) of CRISPR types — weird and poorly understood. There are three subtypes, A–C, and they are ...mechanism of type II is, unsurprisingly, well understood, and the first taught when introducing CRISPR...Covarrubias, P. C., Shmakov, S. A., Silva, C., Tolstoy, I., Johnson, D. B., Koonin, E. V., & Quatrini,...., Hunnewell, P., Alfonse, L. E., Carte, J. M., Keston-Smith, E., Sothiselvam, S., Garrity, A. J., Chong...

...shared resources. As we step into 2025, these milestones remind us of the extraordinary impact that happens...shared progress. But numbers tell only part of the story. This year, we made significant strides in making... D., Cooper, J. A., Crosby, K., Harvey, K. J., Houston, D. W., Kumaran, R., Rego, M., Schofield, C., …...024-01095-8 Kahn, R. A., Virk, H., Laflamme, C., Houston, D. W., Polinski, N. K., Meijers, R., Levey, A....

...have helped Addgene grow and spread our mission.  Boston Museum of Science, where else? Addgenies and other...honored guests headed to the Museum of Science in Boston, where the entire Blue Wing was set aside for the...refresher on the Central Dogma of Biology, I would stop by later this year when it is displayed!).  The ...Joanne shared a moving thank you email from a requestor in Iraq (Addgene's first). After the speeches,...

...maintain high standards for both our depositors and requestors.  Requesting Plasmids available for Packaged ...quality control assays we run with all of our in-stock viral preps: ddPCR, purity testing, sterility testing...AAV2 to be 7x1012, the same titers found in our in-stock preps. If a request has technical considerations...considerations that may affect expected titers, a customer support specialist will reach out to you during the request...

...depositors, used by our requestors, and supported by our repository. You can enjoy these stories, and other 20th...rabies plasmids in our repository to international requestors.    I would be remiss not to mention that 2024...Throughout the year, we’ll be celebrating with stories of innovation and impact made possible by the sharing...

...applications in plants. This smaller, easily customizable vector functions in multiple species, including...for many applications, the plasmid can also be customized due to its modular structure. Two multiple cloning... regulatory elements to be inserted, further customizing gene expression. Sahoo et al. have tested the...Western blotting. Given its versatility and customizability, pSiM24 can be used not only for plant engineering...

...surely what is one of many awards that will be bestowed upon discovers of CRISPR/Cas9 genome engineering...panel discussion with Dr. Craig Mello and Dr. Paul Stoffels from J&J. The panel was moderated by a non-scientist...done with this technology to help patients. Dr. Stoffels and others in the audience discussed how J&J is...Technology? Learn More: Addgene's CRISPR/Cas9 Guide History of CRISPR Cas - A tale of survival and evolution...

...is especially exciting, including histone acetylation by p300, histone demethylation by LSD1, cytosine ... subpages, our updated CRISPR Guide and CRISPR History provide a great overview of the development of ...resources: CRISPR Plasmids and Resources and CRISPR History and Development for Genome Engineering  ...

...patient partners so that they can help tell their stories. I was paired with a six-year-old boy named AJ ...watch fellow Run4Rare team members complete the Boston Marathon, he wanted to run and play with my kids... as efficiently in humans. This is why we cannot stop searching for novel solutions to treat rare diseases...against FOP. Second, Addgene will be hosting the AJ’s Boston League team (Contributing many new heroes to the...

...Nina Dudnik, Founder of Seeding Labs, tells the story about working as a student in a rice lab in the ...supply house.  These large, cheap aliquots could be stored indefinitely at room temperature (thus also saving... powder in 50% nitric acid, but I never let that stop me. I only had to replenish my glass milk supplies... required less agar, less ethidium bromide, less stop buffer and miniscule amounts of plasmid sample.  ...

...indexing strategy that simultaneously tracks clonal history and cell identity at the single-cell level, thus... The CellTags revealed that cells with common ancestors tended to have similar reprogramming outcomes....results suggest that iEP reprogramming is not stochastic, but rather there’s a particular cell state that...track the dynamics of gene expression and clonal history associated with changes in cell identity. The Morris...