...the CNS by lentivirus, which has a larger packaging capacity than AAV. Such an approach was described ...should be screened for activity in vitro and then packaged into AAV for in vivo delivery. Multiplex gene ... also possible and is only constrained by AAV packaging limits (i.e., how many sgRNA expression cassettes...

... target is present immediately upstream of a Protospacer Adjacent Motif (PAM), the sequence NGG.  If these... SpCas9 and its enhanced variants, eSpCas9 and HypaCas9, are great choices. Check out our SpCas9 containing...Cas9 variant with higher specificity (eSpCas9 and HypaCas9) instead.  Choosing a Cas9 for site-specific modifications... within the editing window (bases 4-8 of the protospacer). While this is a small window, this system can...

...lentiviral infection begins with your cells, both your packaging cells and your target cells. Poor tissue culture...harvest after transfecting with the transfer and packaging plasmids. A benefit of the multiple harvest strategy...benefitting your cells, medium exchanges may also impact the quality of the lentiviral particles you harvest...density, volume, incubation time, and MOI can all impact the success of your lentiviral infection. To ensure...

...binding capacity and high protein retention. It comes in a variety of pore sizes and protein capacity, allowing...conjugate will have to be tested to ensure it doesn’t impact the antibody’s binding to your protein of interest... is a little quicker to use, with high binding capacity, though it has lower retention after binding and...10 minutes) and simple, utilizing individually packaged membrane ‘sandwiches’. They work well for proteins...

...take up essentially all of an AAV’s ~4.5 kB packaging capacity. Luckily, researchers have devised methods...the chosen acceptor vector. The vectors are then packaged into AAV virions via transient transfection of...the viral vector. This delivery method frees up space in the AAV allowing a researcher to fill it with...

...many of the same restrictions, like limited cargo space (~4.7 kb) for your genetic payload — there are a... cell types not of interest. Systemic capsids packaging Cre-dependent transgenes can also be paired with...enhancers, and switches all take up precious cargo space in AAV vectors. Dual expression systems, which use...to be administered concurrently to minimize the impact of neutralizing antibodies developed in response...promoter/enhancer screening. However, AAV-PHP.AX packages viral genomes at a lower efficiency than AAV9 ...Zheng, Q., Kozareva, V., Beddow, T., Tobey, I. G., Pacouret, S., Chen, A. T., Chan, Y. A., Ryvkin, D., Gu,...

...rich and diverse workforce. Out of all the career spaces I explored for my post-PhD life, the science policy...webinar via the NC Science Policy student group SciPolPack for a more detailed discussion on how to to define...to chat with someone working in a science policy space you’re interested in. Many fellowship programs can...her work focuses on incentivizing the real-world impacts of health services research. She is particularly...

...recombination between homologous pairs before viral packaging even begins. This spontaneous recombination persists...2020). In general, we observe that 0.1 - 0.8% of packaged viral genomes contain a recombined/functional ...which can lead to off-target expression of the packaged transgene in neurons and glia.   Check the recombination...v14715, titer 2.4x10e13 GC/mL) for this study were packaged at Addgene and had a relatively low recombination...

...lentiviral capsid-based bionanoparticle system for packaging Cas9/gRNA ribonucleoproteins for transient expression...aptamer which binds an aptamer binding protein for packaging into a lentivirus-like particle. Read the paper...paper in Nucleic Acids Research Find the gRNA and packaging plasmids   Cytosine deaminase for RNA editing...

...environmental impact on a scale of 1-10: the lower the score, the lower the environmental impact in terms ... product? How can we consider the environmental impact of those processes? Who even regulates them? You...

...this time more visible and thereby have a positive impact on science. ReFigure saves time, knowledge and ...definitely give you credit for it and increase your impact! Download ReFigure extension and visit ReFigure.org... with Joe Akin. She envisions a world where the impact of teaching and doing science is rapid, concrete...

...the focus shifts to choosing an unexplored and impactful research topic that aligns with your interests...when selecting a research project. What will the impact be in a best case scenario? If you get your dream...what will your paper look like? What will be the impact on your field? You want to choose a research question...

..."CRISPR", short-hand for Clustered Regularly Interspaced Short Palindromic Repeats, in Pubmed returned...want to use double nickase, you could express two spacers and use PX335 to express the Cas9n (nickase). The...usually adds it when they order the oligo. If your spacer sequence starts with a 'G', you naturally have ...

...around the world were closed and not receiving packages. So the entire team mobilized to figure out how...subsequent fluctuations in distribution. I don’t have space in this blog to call out every Addgene Team because...workforce for when we need to be working at full capacity to serve the scientific community. First, we applied...

... here.   Virus production HEKs are the go-to ‘packaging cells’ for viral production because they express...need only transfect the ‘helper’ plasmids (viral packaging machinery) along with the viral plasmid containing...the cargo of interest, and HEKs will produce and package viral particles. The viral particles are then filtered...

... SARS-CoV-2 are the lentiviral production and packaging vectors VSV-G and psPAX2, which were used in a...Repositories around the world facilitate the rapid pace of SARS-CoV-2 research by removing logistic hurdles...and financial support to maintain repositories’ capacity for storing, validating, and distributing such...

...the human interactors we found in our map have an impact on virus replication. While we and our collaborators...one are in a pLVX vector background, so you can package them into lentiviral vectors if you need to. Additionally...get notified!) Attacking coronavirus, one FedEx package at a time Since the publication of our paper, our...

...individually overexpressed and available for lentiviral packaging and delivery to target cells as a pooled library... 2023). Fig. 1: Barcoded TF library was packaged in lentiviral particles and used to transduce ...so it is an invaluable tool to be used in this capacity. Similar to the Human TFome Library, the MORF ...

...Archives allots for 25 MB of space per user, but, if you need more space, you can sign up for a plan. ... by iGreenEarth AMINO is a very simple app that packs important biological information, amino acid abbreviations...

...Biology and, on occasion, would meet in the same space where BosLab exists today. Back then, it consisted...Kendall square, but we saw the potential in the space and decided to reactivate it. We started out by ...projects at BosLab. Some are entrepreneurs using our space to perform early-stage experiments. For example,...