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Showing: 1 - 20 of 78 results

  1. AAV Packaged on Request

    Type
    Collection
    ... Viral Vector Packaging Service Packaged on Request AAV Packaged on Request Overview Details...Discover our AAV Packaged on Request service with all-in-one pricing for viral vector preps, which includes... in-stock capabilities. To meet that need, our Packaged on Request service lets scientists choose from...from over 3,000 transfer plasmids to be packaged in their choice of five serotypes, resulting in over 15,000...15,000 possible choices! By using AAV Packaged on Request, scientists can start experiments as soon as...purify, and titer viral vectors. Overview Our new Packaged on Request service is recommended for research-grade...1 mL, 2.5 mL, or 5 mL). Currently, we can only package plasmids in our collection in the serotypes listed...

  2. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ... Viral Vector Packaging Service Packaged on Request Scope of Service AAV Packaged on Request: Scope of... of Service Addgene's Packaged on Request Service is subject to Addgene’s Terms of Purchase and the applicable...LULLs). Titer For AAV preps produced through our Packaged on Request service, Addgene guarantees a titer...original order. Turnaround Time Production of a Packaged on Request material is generally ready for shipment...

  3. Impact of Genomic Variation on Function (IGVF) Consortium Collection

    Type
    Collection
    ... Depositor Collections IGVF Consortium Impact of Genomic Variation on Function (IGVF) Consortium... a new window) (Link opens in a new window) The Impact of Genomic Variation on Function (IGVF) Consortium...variation affects genome function, which in turn impacts phenotype. The National Human Genome Research Institute...These joint efforts will produce a catalog of the impact of genomic variants on genome function and phenotypes...systematically perturbs the genome to assess the impact of genomic variation on genome function and phenotype...

  4. Zinc Finger Consortium: OPEN Reagents

    Type
    Collection
    ...ID Name 13421 pAC-Kan-alphaGal4 13422 pBAC-lacZ 13424 KJBAC1 strain 21869 pKJ1267 (aka pAC-alphaGal4) 21870... consists of 5 individually packaged plasmids and 3 individually packaged strains, which are shipped as...

  5. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...Virmilion none, need Cas9 plasmid Bullock and Port 49330 pAc-sgRNA-Cas9 dU6 BspQI Transfection Puromycin yes, ...

  6. Retrovirus Plasmids

    Type
    Collection
    ...version of pRX-tight Puro encoding NAT instead of PAC Iglehart 20672 MSCV-IRES-GFP MSCV For transgene expression...γ-Retrovirus Envelope and Packaging Plasmids Common γ-retroviral envelope and packaging plasmids. ID Plasmid...12371 pCL-Eco Packaging Contains MoMLV gag, pol, and env Verma 35614 pBS-CMV-gagpol Packaging MoMLV gag and...retroviral plasmids at Addgene, including popular packaging, envelope, and transfer plasmids. Viral...retroviruses called γ-retroviruses. γ-Retroviruses can package relatively high amounts of DNA (up to ∼8 kb), and...Guide for more information about: γ-Retroviral packaging systems Frequently asked questions (FAQ) about...Plasmid Type Description PI 8449 pUMVC Packaging Contains MoMLV gag and pol; use with envelope plasmid Weinberg...

  7. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...AVAILABLE YET Cre is coexpressed with tdTomato and Pac (puromycin N-acethyl-transferase) pCDH-CB-copGFP-...provided this protocol detailing the process for packaging these transfer vectors and generating infectious... WPRE has been removed to increase the cloning capacity. pCDH-CB-IRES-copGFP-T2A-Puro 72299 Express gene...

  8. Bacterial Expression Systems

    Type
    Collection
    ...repressed by EsaR. Designed to be used with the pAC-EsaR Series . pAC-EsaR Series Various Plasmids pLac lactose... Bacterial two-hybrid assay using pBRα-β flap , pACλCI-β-flap , or their derivatives. Binding between ...protein in pBRα-β flap and the λCI fusion protein in pACλCI-β-flap results in the activation of lacZ expression...

  9. Luciferase Plasmid Collection

    Type
    Collection
    ...imperfect miRNA sequence in the 3' UTR Carl Novina 33154 pAC-Luc Firefly Creation of N-terminal Firefly fusions...Optical reporter of presynaptic ATP Timothy Ryan 24348 pAC-hRluc Renilla Actin5C Insect expression of renilla...

  10. CRISPR References and Information

    Type
    Collection
    ...expression: pAC2 , pAC152 , pAC153 , pAC154 ; pmax dCas9 activator expression: pAC91 , pAC92 , pAC93 , pAC94...Fly: gRNA cloning pAc-sgRNA-Cas9 PDF 165.1 KB Marraffini Bacteria: pCas9 new spacer cloning pCas9 PDF ...pAC94 , pAC95 ; dCas9 activator gateway donors: pAC84 , pAC1 , pAC147 , pAC148 , pAC149 ; gateway destination...destination: pAC90 PDF 1.0 MB Katic Nematode: Cas9 and gRNA use Cas9 (pIK86) ; gRNA empty backbone (pDR274...PDF 125.6 KB Marraffini Bacteria: pCRISPR new spacer cloning pCRISPR PDF 106.7 KB Mendenhall & Myers Mammalian...PDF 248.6 KB Zhang sgRNA cloning lentiCRISPR v2 packaging plasmids: pVSVg , psPAX2 ; positive control: CMV-EGFP...vector system: lentiCas9-Blast and lentiGuide-Puro packaging plasmids: pVSVg , psPAX2 positive control: CMV-EGFP...

  11. Cre-lox system

    Type
    Collection
    ...TagBFP, iCre CAG Mammalian Capecchi 113849 pCAGGS-pac-T2A-iCre iCre CAG Mammalian Capecchi 116879 CAG-Cremyc...Tamoxifen inducible CAG Mammalian Capecchi 125821 pCAGGS-pac-T2A-iCre (v2) iCre and PuroR CAG Mammalian Capecchi...

  12. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...BbsI none S. pyogenes Virmilion Bullock and Port pAc-sgRNA-Cas9 49330 Drosophila BspQI yes, cut S. pyogenes...pLKO5.sgRNA.EFS.PAC 57825 Mammalian/Lentiviral BsmBI none S. pyogenes Puro Ebert pL-CRISPR.EFS.PAC 57828...pyogenes Duchek pAC155-pCR8-sgExpression 49045 Mammalian BbsI none S. pyogenes Jaenisch pAC153-dual-dCas9VP96...vector 51132 Mammalian BsaI none S. pyogenes Huang pAC154-dual-dCas9VP160-sgExpression 48240 Mammalian BbsI...pyogenes tagRFP Ebert pFGC-pcoCas9 52256 Plant AscI, PacI, SbfI yes, cut S. pyogenes Sheen pT7-gRNA 46759 ...pDCC5 59984 Fly BbsI yes, nick S. pyogenes Duchek pAC5-dual-dCas9VP48-sgTetO 48237 Mammalian BbsI yes, ...Lentiviral BsmBI yes, cut S. pyogenes Puro Ebert pL-CRISPR.SFFV.PAC 57829 Mammalian/Lentiviral BsmBI yes, cut S...

  13. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ... of AAV is currently limited by its small packaging capacity (∼4.5 kb including ITRs), though there is...as well as our Packaged on Request service to give you more flexibility. AAV Packaging Plasmids The following...AAV1 AAV packaging plasmid, expressing Rep2 and Cap1 Wilson 104963 pAAV2/2 AAV2 AAV packaging plasmid,... AAV5 AAV packaging plasmid, expressing Rep2 and Cap5 Fan 112863 pAAV2/7 AAV7 AAV packaging plasmid, expressing...AAV8 AAV packaging plasmid, expressing Rep2 and Cap8 Wilson 112865 pAAV2/9n AAV9 AAV packaging plasmid,...Blog: AAV use in CRISPR Viral Vectors 101 eBook Packaging Plasmids AAV Plasmids Adeno-associated viruses...interest and effort directed toward expanding this capacity. Traditionally, AAV requires the presence of another...

  14. Lentivirus Plasmids

    Type
    Collection
    ...along with additional packaging components Trono 63586 psPAX2-D64V 2nd Packaging For making Integrase ...22500 pNHP 2nd Packaging Contains gag, pol, tat, rev Kasparov 1732 pCPRDEnv 2nd Packaging part of the FELIX...strain). Use with packaging construct encoding Tat. Reiser 17531 pCD/NL-BH*DDD 2nd Packaging Contains Gag/...Addgene lentiviral packaging, envelope, and transfer plasmids Viral...Lentiviral Pooled Libraries All Viral Plasmids Packaging Plasmids Transfer Plasmids Lentiviruses, a subset...lentiviral terms Popular Lentiviral Envelope and Packaging Plasmids One safety feature of lentivirus used...multiple plasmids. Some plasmids (typically called packaging and envelope plasmids) encode components of the...

  15. Jaenisch Lab CRISPR Plasmids

    Type
    Collection
    ... Plasmid 48223 pAC91-pmax-dCas9VP64 : dCas9VP64 on pmax expression vector. 48224 pAC92-pmax-dCas9VP96 ...vector. 48225 pAC93-pmax-dCas9VP160 : dCas9VP160 on pmax expression vector. 48226 pAC94-pmax-dCas9VP160...Plasmid 48214 pAC1-pCR8-dCas9VP48 : dCas9VP48 on Gateway donor vector pCR8/GW/TOPO. 48218 pAC84-pCR8-dCas9...TOPO. 48219 pAC147-pCR8-dCas9VP64 : dCas9VP64 on Gateway donor vector pCR8/GW/TOPO. 48220 pAC148-pCR8-dCas9VP96...Activators sgRNA Dual Expression ID Plasmid 48236 pAC2-dual-dCas9VP48-sgExpression : Dual expression construct...dCas9VP48 and sgRNA from separate promoters. 48238 pAC152-dual-dCas9VP64-sgExpression : Dual expression construct...dCas9VP64 and sgRNA from separate promoters. 48239 pAC153-dual-dCas9VP96-sgExpression : Dual expression construct...

  16. Zhang Lab's CRISPR Frequently Asked Questions

    Type
    Collection
    ...want to use double nickase, you could express two spacers and use PX335 to express the Cas9n (nickase). The...PAM sequence to the oligo when cloning my target spacers into the PX330 vector backbone? There is no need...usually adds it when they order the oligo. If your spacer sequence starts with a 'G', you naturally have ... compared to where the target site sequence (protospacer+PAM) is located? If there are other PAMs in the... there needs to be extensive homology of the protospacer followed by PAM. Click here for a target selection... together, with each plasmid having a targeting spacer cloned into the backbone ( pX330 or PX335 , depending...two genomic loci with wildtype Cas9, clone two spacers for each locus into PX330, then co-transfect or...

  17. Genetic Code Expansion

    Type
    Collection
    ...Huiwang Ai 73544 pEvol-pAcFRS.2.t1 pAcFRS.2.t1 E. coli p-acetyl-l-phenylalanine (pAcF) Bacterial TAG Farren... Isaacs 73545 pEvol-pAcFRS.1.t1 pAcFRS.1.t1 E. coli p-acetyl-l-phenylalanine (pAcF) Bacterial TAG Farren...141173 pAcBac1-3nitroY-A7-RS A7 3nY tRNA synthetase M. barkeri Mammalian Ryan Mehl 141174 pAcBac1-haloTyrRS...acid Bacerial, Mammalian TAG Peter Schultz 50831 pAcBac2.tR4-OMeYRS/GFP* tyrosyl-tRNA synthetase E. coli... amino acids Mammalian TAG Peter Schultz 50832 pAcBac1.tR4-MbPyl PylRS M. barkeri variety of unnatural...barkeri Tetrazine 3.0 Bacterial TAG Ryan Mehl 174081 pAcBac1-NES-Flag-R2-84-MbRS Tetrazine3.0 NES-Flag-R2-84...barkeri acridone Bacterial TAG Ryan Mehl 174101 pAcBac1.tR4-AcdRS82 Acridone 82 tRNA synthetase M. barkeri...

  18. CRISPR Guide

    Type
    Collection
    ...referred to as CRISPR ( C lustered R egularly I nterspaced S hort P alindromic R epeat) technologies. Before...for Cas-binding and a user-defined ∼20-nucleotide spacer that defines the genomic target to be modified....target is present immediately adjacent to a P rotospacer A djacent M otif (PAM) The PAM sequence (NGG)... an active, DNA-binding configuration, with the spacer region of the gRNA left free to interact with the...Cas9 will only cleave a given locus if the gRNA spacer sequence shares sufficient homology with the target...throughout the genome, called off-targets, that can impact your experiment. There are many online tools available...Cas9’s interactions with DNA phosphate backbone HypaCas9 - increase Cas9 proofreading and discrimination...

  19. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...contains many unique protospacer sequences that have homology to foreign DNA. Protospacers are separated by...Adaptive Immune System CRISPR (Clustered Regularly Interspaced Short Palindromic Repeat) sequences were initially...contain a species-specific sequence known as a protospacer adjacent motif (PAM). The CRISPR complex binds...sequence is only valid if it contains a special Protospacer Adjacent Motif (PAM) directly after where the... development has occurred at a truly astonishing pace, with work directed towards increasing on-target...fidelity Cas9 variants , SpCas9-HF, eSpCas9, and HypaCas9 display very low off-target activity due to rationally...SaCas9) is smaller than SpCas9 and more easily packaged in AAV. Cas9 orthologs also have distinct PAM ...

  20. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...consist of a 20-mer (“protospacer sequence”) upstream of an “NGG” sequence (“protospacer adjacent motif” or...Abstract The prokaryotic clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated...Figure 2A ). NOTE: In this example, sgRNA-A’s protospacer sequence and PAM happen to fall on the bottom...bottom (Crick) strand while sgRNA-B’s protospacer sequence and PAM fall on the top (Watson) strand ( Figure...will occur independent of orientation of the protospacer sequence/PAM relative to the top or bottom strand...However, if the first position of the 20-mer (protospacer sequence) is G, do not add another G ( e.g., ...cleavage site to ensure detection would not be impacted by a small indel at the sgRNA target site. Design...
Showing: 1 - 20 of 78 results