...plasmids in the library.  Spinfection If you have a large number of cells to transduce, you may want to consider...pooled libraries can be intimidating and require a large amount of experimental work, but with this guide...protocol. After functional titer is determined, larger lentiviral preps can be used for screening-scale...

...pathways and represent the largest family of human drug targets. To enable large-scale profiling of many ...by endogenous GPCR. Example applications include large-scale pharmacological profiling or interrogation...

...lentiviral-based CRISPR libraries available at Addgene. The large packaging capacity and stable expression of lentiviral... vivo CRISPR screens. AAV vectors have played a large role in these advancements, due to their many benefits...of molecular pathways. These screens have gotten larger, easier, and more comprehensive thanks to the consistent...

...the University of Goettingen, and several other large research institutions. The following graphic depicts...institutional and lab servers specifically designed to hold large amounts of data. Although it may be useful to selectively...

...novel drugs. Commercial Affairs teams make up a large and diverse group of professionals running the marketing...challenging. For instance, the client might need to get a large amount of market research done to inform a go/no-go...

... fused to a dimerizing FP has potential to form large stable polymers of dimers. 3) FRET biosensors that...dimerizing FP is fused to the POI. These structures are large (microns), bright, non-physiological, and difficult...

...which is formed by a complex of two proteins: the large (L) catalytic protein and its cofactor VP35. VP30...necessary control elements for each would be relatively large. Cloning the viral genes in this plasmid would be...

...datasets are too large to transfer to a journal repository, they are likely too large to be retrieved ...their own data, with some smaller labs feeling that larger, better resourced, groups would harvest and reanalyze...

...ambitious projects that require epitope tagging of a large cohort of genes (e.g. tagging genes representing...success of the epitope tagging experiment depends largely on how efficiently these components are delivered...immense possibilities to delineate the function of large protein cohorts in diverse mammalian cell types....

... chemical synthesis of these molecules. To make large amounts of pure product in an affordable way, researchers...that can be assembled into individual TUs or very large (up to 20!) multi-TU constructs (Moore et al., 2016...needed in mammalian cell types, and comes with a large library of common parts to get you started quickly...build genetic circuits and would benefit from a larger library of parts but don’t need to add tags to ...multiplexed CRISPR, and chromosomal integration of larger expression cassettes (Shaw et al., 2023). Those...

... a patent counsel and ran a group at two fairly large companies, Genzyme and Biogen. They’re very different...

... the user in different formats. This can have a large impact on downstream results. Examples: “HTML”, ... agnostic”, “model organisms”, “small genome”, “large genome”, and “organism biased scoring”. Trends: ... CRISPR design (I’m talking screens and/or very large genomes), then it is worthwhile buying a dedicated...

...deliver larger cargo such as base editors and prime editors. While using AAV to deliver large transgenes... with split AAVs. In general split AAVs break a large transgene in two pieces and package each piece into...

...offering temperature-sensitive materials, we noticed a large percentage of these shipments arrived in India at...expertise in a specific country can quickly reduce a large number of barriers. Though we haven’t solved every...

...reconstitution via mRNA trans-splicing. By splitting a large gene into smaller fragments, delivering them by ...prime editing, and delivery of disease-relevant large proteins. They demonstrated REVeRT in various contexts...

...Reddit. Here are some reasons why: 1. Reddit has a large and active community of people interested in science... moderators did not delete or suppress were the large number of scientist posters with serious career ...

... to its efficient use in screening experiments; large amounts of virus are required to ensure that you...

...open-access. Though it is time for me to refill my large coffee cup and get back to our booth, I will have...

...colonies that are comprised of cells with a large nucleus, large nucleoli, and little cytoplasm. Positive...other methods. This could be a reflection of the large size of the plasmid or silencing of the plasmid ...oncogenes, such as Myc. Viral vectors also have the largest genomic footprint of iPSC generating methods due...infect dividing and nondividing cells and have a larger cloning capacity (8-10 kb) than other retroviral...transfection efficiency is cell-type dependent and larger plasmids have lower rates of transfection. Minicircles...

...destined to continue in academia. It seems it’s largely a matter of luck if a student or postdoc ends up...