Addgene

We narrowed to 178 results for: DAP

Showing: 101 - 120 of 178 results

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Learning Technical Skills at a Lab Central Workshop

Type

Blog Post

Published

Feb. 20, 2018, 2:30 p.m.

...using this kit is: Fragmentation and end repair Adapter Ligation Clean up/Size selection Amplification ...
Visualizing Translation at the Single Molecule Level

Type

Blog Post

Published

Aug. 1, 2017, 1:15 p.m.

...disrupting ribosome-mRNA complexes. Recent work has adapted this technique to monitor local translation and...
Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

Type

Blog Post

Published

Nov. 20, 2018, 8:17 p.m.

... that exist online, but this approach has been adapted for the identification of physical materials that...
Choosing Your Fluorescent Proteins for Multi-Color Imaging

Type

Blog Post

Published

Oct. 9, 2014, 3 p.m.

...and RFP or the Sedat Quad filter set, designed for DAPI / Fluorescein / Rhodamine / Cy5 (e.g. Semrock's)...
Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

Type

Blog Post

Published

May 19, 2020, 1:15 p.m.

... in much of the world, we have found ourselves adapting to a new way of doing things. These new processes...
Hot Plasmids - February 2022

Type

Blog Post

Published

Feb. 22, 2022, 2:15 p.m.

...mitochondrial (mito-mCherry-1xFLAG) localization. Adapted from Liu et al. 2021. Anti-FLAG and anti-HA...
Antibodies 101: Isotypes

Type

Blog Post

Published

Oct. 19, 2021, 1:15 p.m.

... constant domains. This switching helps B cells adapt during the course of an immune response since it...
Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

Type

Blog Post

Published

June 6, 2019, 12:41 p.m.

...group inserted sgRNAs targeting various innate and adaptive immune genes into pXPR_053. They expressed this...
A Deep Dive into BioBuilder

Type

Blog Post

Published

Oct. 11, 2017, 1:44 p.m.

...with synthetic biology lab kits and lesson plans adapted from her own MIT curriculum. Drawing on current...
Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

Type

Blog Post

Published

July 28, 2017, 1:30 p.m.

...cell differentiation (a cell type important for adaptive immune responses, which flies do not have!) (4...
Luminescent Imaging with Nano-lanterns

Type

Blog Post

Published

May 25, 2017, 2:30 p.m.

...Nano-lantern, showing the technique’s versatility and adaptability to many different contexts. Improving Nano-...
CasPEDIA: A Functional Classification of Cas Enzymes

Type

Blog Post

Published

Jan. 25, 2024, 2:15 p.m.

...Comparison of different Cas enzymes using CasPEDIA. Adapted from Adler et al. You can also search by ...
Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

Type

Blog Post

Published

Nov. 5, 2019, 1:28 p.m.

...years flew by, but since then, scientists have adapted CRISPR nucleases for many more specific research...
Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

Type

Blog Post

Published

June 2, 2015, 6:06 p.m.

...designed for plant expression, PTGs will likely be adaptable to many different biological systems. Although...
Plasmids 101: The protein expression toolbox

Type

Blog Post

Published

Feb. 16, 2023, 7:05 p.m.

... Fig. 3 dTag mechanism of protein degradation. Adapted from Nabet et al. Check out Addgene's Lentiviral...
Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

Type

Blog Post

Published

March 19, 2019, 1:31 p.m.

...sequence TOM20 (pTriEX Tom20 mVenus BoPD). In dark adapted cells expressing these plasmids, tgRFP fluorescence...
Summer SciComm: Preprints

Type

Blog Post

Published

Aug. 16, 2022, 1:15 p.m.

...asapbio.org/preprints-and-covid-19 Ettinger CL, Sadanandappa MK, Görgülü K, Coghlan KL, Hallenbeck KK, Puebla...
Plasmids 101: Cre-lox

Type

Blog Post

Published

Jan. 13, 2015, 3:47 p.m.

...natural viral life-cycle, and researchers have adapted the components for use in genome manipulation. ...
Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

Type

Blog Post

Published

Dec. 14, 2021, 2:15 p.m.

...methylation, leaving the target CpG unmethylated. Adapted from Sapozhnikov and Szyf 2021. Let me leave...
Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

Type

Blog Post

Published

Aug. 24, 2021, 1:15 p.m.

...indirect ELISA (more details below) can also be adapted to a competitive format. Figure 2: Overview...

Showing: 101 - 120 of 178 results

Results per page:

Learning Technical Skills at a Lab Central Workshop

Visualizing Translation at the Single Molecule Level

Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

Choosing Your Fluorescent Proteins for Multi-Color Imaging

Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

Hot Plasmids - February 2022

Antibodies 101: Isotypes

Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

A Deep Dive into BioBuilder

Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

Luminescent Imaging with Nano-lanterns

CasPEDIA: A Functional Classification of Cas Enzymes

Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

Plasmids 101: The protein expression toolbox

Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

Summer SciComm: Preprints

Plasmids 101: Cre-lox

Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

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