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  1. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

    Type
    Blog Post
    Published
    June 19, 2018, 1:38 p.m.
    ...bacteria and archaea to regulate their buoyancy. The Shapiro lab recently discovered that gas vesicles also ...Lakshmanan, A., Farhadi, A., Kumar, S.R., Nety, S.P., & Shapiro, M.G. (2018). Acoustic reporter genes for noninvasive...

  2. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

    Type
    Blog Post
    Published
    Sept. 12, 2023, 1:15 p.m.
    ...molecular biology tools not listed in today’s post? Share them in the comments below. This post was written...software for your needs 5 great apps for lab life  Sharing your lab protocols using apps to save you time ...

  3. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

    Type
    Blog Post
    Published
    June 29, 2023, 7 p.m.
    ... who need it!  After a few months, we wanted to share these, and other, findings with the larger content...something that was challenging while helping us share our resources better with the community and knowing...

  4. Hot Plasmids - October 2022

    Type
    Blog Post
    Published
    Oct. 6, 2022, 1:15 p.m.
    ...channelrhodopsins by: Mike Lacy New work from the labs of Mingshan Xue and John Spudich identifies a novel class ...10.1038/s41593-022-01094-6. (This work was also shared as a preprint: https://doi.org/10.1101/2021.09.17.460684...

  5. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    Published
    Nov. 17, 2016, 3:30 p.m.
    ...researchers we’ve met with have asked if we can help them share and distribute other reagents. Virus distribution...continue to develop it. More communication and sharing will only help research advance. Remember: productivity...

  6. Identifying Sequence Elements with SnapGene's Feature Database

    Type
    Blog Post
    Published
    Dec. 21, 2017, 2:06 p.m.
    ...that SnapGene supports “Collections”, which are shareable databases of DNA and protein sequences, the next...organization can annotate their sequences from a shared database of custom common features. Second, customers...

  7. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    Published
    June 9, 2022, 1:15 p.m.
    ...include age range, familiarity with the subject, shared interests, socioeconomic and/or racial/ethnic background...Additional resources on the Addgene blog 10 Ways to Share Your Science Science Communication - 9 Ways to Get...

  9. What the HEK?

    Type
    Blog Post
    Published
    April 18, 2023, 1:15 p.m.
    ...qualities, which is inconsistent with kidney cells (Shaw et al.). Since then, transcriptome studies of the...the HEK not? References and Resources References Shaw G., et al., Preferential transformation of human...

  10. Summer SciComm: Science Storytelling

    Type
    Blog Post
    Published
    Sept. 16, 2022, 1:15 p.m.
    ...communication and can use what you've learned to share your science with others.    My favorite quote about...sense of curiosity and wonder why you’re doing and sharing science in the first place?   References and Resources...

  11. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    Published
    Jan. 21, 2014, 3:08 p.m.
    ...outside of the traditional academic setting and to shape and strengthen interests and skills. Although the...your lab bench.  Want further information about shaping your career to your values, interests, and skills...

  12. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    Published
    Aug. 2, 2018, 12:56 p.m.
    ... a classroom presents equipment needs, such as shakers and incubators, and biosafety issues, such as how...build an open-source community of users who can share their experiences and knowledge with each other ...

  13. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    Published
    April 9, 2020, 1:15 p.m.
    ..., our group has been inundated with requests to share our plasmids with other researchers. We have been...requests and tracking their fulfillment manually in a shared spreadsheet and it takes time away from our research...

  14. Early Career Researcher Toolbox: Free Tools for Science Writing

    Type
    Blog Post
    Published
    April 28, 2021, 1:15 p.m.
    ... you can access it from anywhere, and it can be shared with others. The Web Importer plug-in lets you ...Files can be synced across devices, and you can share your library with others which makes it easier to...

  15. Advice for Scientists Starting a Lab

    Type
    Blog Post
    Published
    April 29, 2014, 1:43 p.m.
    ...counter-productive." He also emphasizes the point to “encourage sharing - in part by having projects ambitious enough that...you have any relevant tips for starting a lab. Or share your success stories below. And don't forget to ...

  16. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    Published
    Feb. 2, 2021, 2:15 p.m.
    ...eligibility, interest, and career fit. Most internships share a common set of criteria such as citizenship status...that will also benefit from these opportunities. Share the resource, and apply as a cohort. This may sound...

  17. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    Published
    Aug. 20, 2024, 1:15 p.m.
    ... Negative and positive samples are clearly distinguishable as two separate populations. Created with biorender.com...She is currently a Research Assistant in Arlene Sharpe’s lab at Harvard Medical School.   More resources...

  18. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type
    Blog Post
    Published
    Feb. 18, 2014, 4:22 p.m.
    ...navigate career changes and overcome barriers by sharing broader perspectives and holding one another accountable...perspective. Sometimes it is helpful to find peers that share a similar direction or problem. For example, a group...

  19. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    Published
    Jan. 31, 2019, 1:32 p.m.
    ... pathological active regions, microenvironments shape a tumor’s evasiveness, evolution, and response to...always interacting with its surroundings and being shaped by them. Another set of highly influential microenvironment...

  20. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    Published
    April 10, 2018, 12:56 p.m.
    ...but they are laborious to generate and costly to share. What is a RANbody? A RANbody is a fusion between.... Staining with multiple HRP-RANbodies is indistinguishable since they produce the same signal, so only...

  21. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    Published
    April 14, 2022, 1:15 p.m.
    ...either simultaneous or independent of the other (Shahied, 2004). They are often used for clinical applications...antib8020028. PMID: 31544834; PMCID: PMC6640713. Shahied, L., et. al. (2004). Bispecific Minibodies Targeting...

  22. Antibodies 101: Fab Fragments

    Type
    Blog Post
    Published
    Sept. 5, 2024, 1:15 p.m.
    ...Imagine an antibody. Do you immediately visualize a Y-shaped protein reminiscent of the Addgene mascot Abi? ...chain. When assembled, the four chains make a Y-shaped protein that can be divided into the Fragment Crystallizable...

  23. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    Published
    April 12, 2016, 2:30 p.m.
    ...2015; Koike-Yusa et al., 2014; Ma et al., 2015; Shalem et al., 2014; Wang et al., 2014), knock down (Gilbert... 26190106. PubMed Central PMCID: PMC4559080. 8. Shalem, Ophir, et al. "Genome-scale CRISPR-Cas9 knockout...

  24. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    Published
    Jan. 14, 2016, 3:30 p.m.
    ...One of the best things about sharing plasmids through Addgene is that we provide an added level of confidence...Serial Cloner, and Snapgene), and save these to a shared computer or server. This will ensure that future...

  26. How to Prepare for an Industry Interview

    Type
    Blog Post
    Published
    July 9, 2020, 1:15 p.m.
    ...stories to answer interview questions. Story telling shares many of the same elements as a STAR answer: a setting...questions can be hard during this talk since you’ll be sharing your screen and won’t be able to see who’s asking...

  28. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    Published
    Jan. 5, 2021, 2:15 p.m.
    ...competing mobile genetic elements. Image by Basem Al-Shayeb in the Doudna Lab. Scientists looking for more...your experiments? AAV-flex-taCasp3-TEVp from Nirao Shah's and Jim Well's lab induces cre-dependent apoptosis...

  29. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    Published
    Oct. 22, 2019, 12:20 p.m.
    ... readers to more information if they desire it. Shapes. In this type of poster, written and graphical ...trickier to make figures fit nicely into circular shapes rather than a square or rectangular boxes. Background...

  30. Tips for Technical Support Calls

    Type
    Blog Post
    Published
    Sept. 20, 2016, 2:39 p.m.
    ...help emails and phone calls, I feel compelled to share my years of accumulated wisdom so that you, the ...concern is based on data you have produced, please share the data. Maybe not all, but many bio companies ...

  31. Gaining Leadership Skills Volunteering at a Professional Organization

    Type
    Blog Post
    Published
    Aug. 6, 2015, 2:30 p.m.
    ...Vision and Ophthalmology Research (ARVO). Hopefully sharing my experience will show you how you can get started...and benefited greatly by improving my skills and sharing with other more junior leaders what I had experienced...

  32. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    Published
    June 5, 2014, 3:32 p.m.
    ...can rely on one another to celebrate good news or share a bottle of wine after a rough day, and we have ...making the switch from academia to entrepreneurship, share your thoughts with us in the comments below. We’...

  34. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ...that matures quickly and the other slowly, see Verkhusha et al.), one benefit of the common FP timers is...19889833. PubMed Central PMCID: PMC2801723. 3. Verkhusha, Vladislav V., et al. "An enhanced mutant of red...

  35. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ...been used for protein expression. Plant systems share many of the same features and processing requirements...loves being able to help the scientific community share plasmids.   Additional Resources Thermofisher Protein...

  36. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    Published
    May 22, 2015, 4:58 p.m.
    ... technology: because they necessarily alter the shared environment, they must never be used without popular...associated ecosystems.  Because the environment is shared by everyone, he is deeply concerned with ensuring...

  37. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    Published
    July 16, 2020, 1:15 p.m.
    ...False, type='scriptV4', hide_playlist=True, viral_sharing=False, embed_button=False, autoplay=False, hidden_controls... on the Addgene blog Learn more about plasmid sharing during COVID-19 Resources on Addgene.org Find...

  38. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ...designing the best donor DNA, and avoiding common mishaps in this class of genome edit. Knock-in mutations...2020). 10.1016/j.jbiotec.2019.11.010 Wright, D. W., Shah, S. S., Heyer, W. D. Homologous recombination and...

  39. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    Published
    May 15, 2014, 3:33 p.m.
    ... 1996, is an eleven β-sheet-containing “barrel” shape, with the chromophore concealed at the center of...Gal Haimovich of greenfluorescentblog. Gal will share his list of 10 things to keep in mind when choosing...

  40. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ...at the limited number of hits. So, here, I will share my process with you. But before I do that, let me...formatting guidelines, etc.) you have some criteria to shape the document. Get and use a reference management...
Showing: 201 - 240 of 490 results