...used in experiments. Retroviral vectors make up a large toolbox used by researchers for (among other things...

...in this educational video. “SLC6A8 belongs to a large family of solute channels that are responsible for...

...many samples you may have, access to one or more large, expensive ultracentrifuge.  Figure 1: An ...

...communication has helped Addgene move from completing 2-3 large projects a year, on average, to completing 8-10 ...

... can be excited many times, thereby producing a large number of emitted photons before they are destroyed...

...to be large enough to target larger areas, typically a few hundred nl. Alternatively, for larger brain...lentiviral vectors are superior packaging vectors for larger genes - they can hold up to 8 kb, while AAV can...

...) and even on 60 Minutes. We are blessed with a large community of scientists who support us by depositing...

...microscope showed us that, in monkey neurons, the large majority of the gold particles were in the cytoplasm...after it has been administered. Although we found a larger proportion on the membrane in mice neurons, most...

... lab management software aren’t just limited to large volume facilities; it can be useful in academic ...

...CRISPR guide RNAs (gRNAs) for genome editing in large-scale has led to the rapid generation of genome-...

..., two full antibodies bound together may be too large. Using a complex that is one full antibody bound...and have lower binding affinity than some of the larger fragments.  Think of antibody fragments as new ...

...germline editing. Although this paper represents a large step forward in the CRISPR field, it’s important...

...of fragments, or the ligation of very small/very large fragments, these problems can be overcome by screening...

...indicating that Met protein functions have been largely conserved since the species diverged. Tips on using...

... commonly observed with direct stimulation are largely absent in the soCoChR-expressing system. Taken ...

...Steitz was presented with a physical model of his large ribosomal subunit, he kept sliding his palm across...the models we create are around 30 million times larger than the real thing. Nevertheless, the models are...

... and time-consuming to generate, especially for large animals such as primates. Viral vectors with cell-type...

...sequence ranging from a single nucleotide change to large insertions like the addition of a fluorophore or...

...the possibilities can seem overwhelming given the large assortment of fluorescent proteins available. Fortunately...

[email protected]. These deposits are considered large deposits and are exceptions to our standard workflow...

...the conference you are attending on the larger side? At large conferences, chances are that several talks...

...friendly, to own the process entirely remains a large undertaking. Priority number one was to ensure that...regions that are GC rich. Since NGS assembles a larger sequence from many smaller sequences, regions with...

...cells with unique spatiotemporal phenotypes from large heterogeneous cultures.  HeLa cells expressed...without any reduction in performance compared to larger genome-wide libraries. Find the library here ...

...at two sites within the viral genome to excise a large portion of it. This ongoing trial is sponsored by...

...you want to use in a given experiment. For very large panels (upwards of 30-40 colors), spectral flow ...particularly useful tool for those wishing to run larger panels: five laser spectral flow machines have ...fluorophores. This allows for the creation of much larger panels, as fluorophores that cannot be distinguished...

...could be good choices if you have a particularly large number of inserts to assemble. You could also consider...

...CRISPR variety (see slides from my talk below). Largely organized by Dmitry Samarsky, and a very welcoming...

...in my lab notebook from time to time. I wrote in large letters “Going for it!” We kept our day jobs and...

...Conversely, if your protein of interest is large, look for a larger pore size (0.45 µm).  Types of transfers...to overnight to run, and is recommended for very large proteins. A semi-dry transfer is faster — 3–30 minutes...

...can be produced at high titers, can accommodate large transgenes, transduce quiescent and dividing cells...

...system, the mutagenesis plasmid (MP6) permits quick, large-scale mutation of the gene of interest. They created...

...believe this isn’t cause for concern. However, a large number of hits to one sequence/gene - we set our...

... advantage of this technique is the potentially large number of single clones obtained (on occasion we...

...relies on antibody chains, which are relatively large and are not expressed consistently throughout cells...

...interest from relevant parties, and the company at large, by polling and informal communications we made ...winding down. Perhaps other projects have taken on a larger priority for the participants. Perhaps people feel...

...and actual molecular size – it is possible that a large or charged FP fusion can change the subcellular ...

...gRNAs Importantly, the use of truncated guides has largely been demonstrated to result in decreased mismatch...

...you are interested in viewing which has drawn a large crowd, making it difficult to see the actual poster...posters you are interested in viewing. For most larger conferences, poster titles and abstracts are available...

... homology directed repair (HDR), and generating large genomic deletions. What are the advantages of Cas9...

...microscopy) Visually compelling. Easy to convey a large number of details without overwhelming your audience...