Skip to main content
Addgene
Showing: 81 - 100 of 102 results

  1. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ...too young and should not be used for important transfection experiments right after they’ve been thawed....

  5. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...electroporation or poor cellular entry with other transfection methods. The Junwei Shi Lab addressed this problem...

  6. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    ...restriction digest! Then, you can transform or transfect the cells that you want to use for your experiments...

  7. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...structures within cells. HeLa cells were transfected with plasmids encoding FPs tagged with the plasma...

  8. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ...could interfere with interpreting the results of transfection assays. Go forth and perform your DNA extractions...

  9. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    Published
    Dec. 7, 2021, 2:15 p.m.
    ...protein of interest, such as a sample transiently transfected or stably infected with the protein of interest...

  10. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...scientists use a multitude of techniques including co-transfection of two or more plasmids, the use of multiple...

  11. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    Published
    April 20, 2021, 2:30 p.m.
    ...plasmid, and (3) the packaging plasmid. These are transfected into target cells which produce lentiviral particles...

  12. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    Published
    Nov. 3, 2022, 12:15 p.m.
    ...optimizing this step, or consider enriching for transfected cells by flow sorting or drug selection, assuming...

  13. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    Published
    Jan. 3, 2019, 1:35 p.m.
    ... as early as 48-72 hours post-transformation/transfection in a transient assay. For stable transformation...

  14. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...genome. While this may effectively serve as a transfection or transduction control, a different gRNA would...

  16. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ... With the ability to produce neuron specific transfection using Cre-lox, this new method could allow for...

  18. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    .... But that doesn’t mean you have to clone and transfect multiple promoter-gRNA constructs in order to ...

  19. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    Published
    March 25, 2025, 1:15 p.m.
    ...HMCES, either as a fusion construct or by co-transfection. HMCES forms a chemical crosslink with DNA abasic...

  20. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    Published
    Feb. 23, 2016, 3:30 p.m.
    ...PITCh-gRNA binding sites. This PITCh plasmic is cotransfected with a plasmid carrying Cas9, the PITCh-gRNA... containing Cas9 and the PITCh-gRNA. Step 3: Contransfect the PITCh vector with the vector carrying Cas9...
Showing: 81 - 100 of 102 results