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  3. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    Published
    Feb. 18, 2016, 3:42 p.m.
    ...you should send it for sanger sequencing using primers that will allow you to read over the insert. See...

  5. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ...Polymerase Chain Reaction (PCR) by using a biotinylated primer. Biotin can also be enzymatically incorporated ...

  9. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...select the appropriate pair of vector and gene primers from Table 2 of the associated publication which...of sequence homology between the vector and gene primer for SLIC cloning. Amplify the pCoofy vector and...

  12. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    Published
    Feb. 27, 2024, 2:15 p.m.
    ... was inserted in the plasmid backbone and what primers can be used to verify it, as shown below:   Figure...

  15. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...encodes a unique restriction enzyme site and unique primer sites for more rapid screening by restriction digest...

  16. CRISPR 101 eBook...en español

    Type
    Blog Post
    Published
    Jan. 18, 2024, 2:15 p.m.
    ...¡La primera edición de nuestro libro electrónico CRISPR 101 ya está disponible en español! Esta traducción...

  17. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    Published
    June 9, 2022, 1:15 p.m.
    ...absence of a predefined DNA sequence that uses trprimers and enzymes that will amplify a sequence only ...
Showing: 81 - 97 of 97 results