...quote Ryan Mehl, PhD, GCE has “10x the impact of PCR. But it’s 100x more complicated.” And Ryan Mehl would...

...integrates our database of over 2000 primers. Two 96-well PCR plates are prepared with the primer stocks for forward...

...nuclear receptor and coregulator research. Our Q-PCR datasets, for example, document the expression patterns...

... a blood draw, tail clip, or toe clip) and using PCR with the appropriate primers to detect your targeting...

...of a downstream application such as quantitative PCR to assess gene expression. There are several things...

...Development and optimization of a real-time quantitative PCR-based method for the titration of AAV-2 vector stocks...

...reaction. This will allow you to quantify the amount of PCR product that comes from amplification of the original... titer are direct p24 measurements via ELISA and qPCR for viral RNA. Measuring p24 levels in a viral prep...first converted to cDNA and then quantified using qPCR primers targeting specific viral components such...assays depending on the vector backbone. When using qPCR based methods for viral titration consider using...most accurate method of lentiviral titration uses qPCR to measure the number of proviral copies that have...cell line known to contain 1 integrated copy of the qPCR target gene. Developing the proper control line ...

...screen for this specific recombination outcome using PCR and then generate a monoclonal cell line with the...

... have DNA left over for other applications, like PCR, cloning, transfection, or long-term storage. But...

...insertions/deletions using a mismatch repair assay? Or is PCR followed by gel electrophoresis and/or next-generation...

...the purified virus should be ~1.3 Quantitative PCR: the number of viral DNA packaged in virions is determined...

...efficacy, we have used both Western blotting and PCR, depending on the level of endogenous expression....

...it’s so easy once you are in the lab. There is no PCR, no gel purification; the whole cloning process is...

...both edited and unedited (control) populations is PCR-amplified and Sanger sequenced. ICE compares these...

...bound genomic locus, which they then confirmed by PCR. Subsequent mass spectroscopy studies allowed for...

...Ehrhardt, A., & Baiker, A. (2012). Universal real-time PCR for the detection and quantification of adeno-associated...aliquots of virus. AAV titration by qPCR Overview: AAV titering by qPCR quantifies the number of genome-containing... hours for the qPCR run and data analysis. Refer to this Addgene AAV Titration by qPCR Protocol for more...remember to make one or two small aliquots to use for qPCR titering. This helps avoid multiple freeze/thaws... included. While the physical titer generated by qPCR is useful, I test a few different concentrations...

... selection. Screening of transformants by colony PCR confirmed the insertion of the Lambda insert at the...

...titrated following purification using qPCR or digital droplet PCR. Insect cells Insect cells are a less...

...plate overnight Screen colonies phenotypically or by PCR/sequencing While CRISPR-transposons are powerful...

...detected directly by Northern blot analysis or RT-PCR. Uses of the minigenome system Minigenome systems...