We narrowed to 232 results for: pET

...particularly enriched in highly transcribed regions and repetitive regions, such as telomeres and centromeres. These...presumably due to their abundance of R-loops and repetitive sequence. All of the R-loops described above...

... formation of replication competent lentivirus (RCL). Replication competence can arise from recombination...

... get on stage for a Science Slam – a sort of competitive TED talk – to win the audience’s applause with...photography and participate in a science photo competition. Or nominate yourself as an educator for a short...

...into the host genome, the virus is replication incompetent, meaning it can’t replicate and continue the ...Viral cargo-specific safety   After replication competency, the second concern with virus safety is the ...

... a team at UVic to compete in iGem, an MIT-sponsored synthetic biology competition. In 2010, he founded...

...70% ethanol immediately. Finally, avoid sticking pipettes into solution bottles directly, rather decant ...material that is intended for reuse (e.g. Pasteur pipettes for liquid waste) should be sterilized before ...

...CRISPR-transposons by the Sternberg and Zhang labs (Peters et al., 2017; Klompe et al., 2019; Strecker et ...225 . https://doi.org/10.1038/s41586-019-1323-z Peters JE, Makarova KS, Shmakov S, Koonin EV (2017) Recruitment...

...Prashers will find it increasingly difficult to compete (for funding, etc.) with that produced by the confident...the U.S. environment, and a decline in the competitiveness of U.S. science and technology.” This last ...

...division. These non-plasmid-containing bacteria can compete with and confound the goal of your experiment —...express the Tn5 element, but thankfully, most competent cells do not express this factor. Zeocin is handy...

...virions) are not expressed. Note that replication-competent AdV vectors also exist and are used in certain...topic, which is that natural AdVs, replication-competent viruses with a recombinant genome, and adenoviral...

...system, the possibility of creating a replication-competent virus via recombination between the transfer and...recombination events necessary to create a replication-competent virus. Second, Tat is eliminated through the addition...

...single colony with a sterile flat toothpick or pipette tip and swirl in a small amount of sterile water...loading dye with glycerol to your samples before pipetting them onto the gel. The figure above summarizes...

...replication that can be packaged into replication-incompetent phage particles using a helper system. These ...antimicrobials combines them with both replication-competent phages and antibiotics. Phage therapy fell out...

...each other, dressing in costume, and holding competitions (ranging from cook-offs, to corn toss to trivia...enjoyed some friendly -or in some cases, serious-  competition with our corn toss boards, Kan Jam, and Ladder...

...intense amount of competition between microorganisms for available resources. This competition drives microbes...

...residue at the N terminus with a small peptide (LPETG). In EXCELL, the mgSrtA variant can be displayed...interacting cell surface proteins with a biotin tagged LPETG peptide. Biotin tagged proteins can then be purified...

...References March for Science Website Locke, John, and Peter Laslett. Locke: Two treatises of government student... 15-008: Perspectives on Broader Impacts. The Pipettepen: The March for Science Raises Concerns Over Politicization...

...levels of assertiveness and cooperation: avoiding, competing, accommodating, collaborating, and compromising...Though there may be a time and place for avoiding, competing, or accommodating, if you find yourself gravitating...

...controls help you rule out errors like uneven pipetting or irregular membrane transfer. Figure ...important for semi-quantitative western blots. No pipetter is perfect, and proteins may not transfer precisely...

...-mimetic sequences that imitate this motif and compete with both small molecule integrin inhibitors and...their targeted integrin subtype, even when in competition with other similar integrins, as reported by ...