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Showing: 81 - 100 of 219 results

  1. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    Published
    June 22, 2023, 1:15 p.m.
    ...into the host genome, the virus is replication incompetent, meaning it can’t replicate and continue the ...Viral cargo-specific safety   After replication competency, the second concern with virus safety is the ...

  2. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ...70% ethanol immediately. Finally, avoid sticking pipettes into solution bottles directly, rather decant ...material that is intended for reuse (e.g. Pasteur pipettes for liquid waste) should be sterilized before ...

  3. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ...system, the possibility of creating a replication-competent virus via recombination between the transfer and...recombination events necessary to create a replication-competent virus. Second, Tat is eliminated through the addition...

  4. Plasmids 101: Colony PCR

    Type
    Blog Post
    Published
    May 12, 2016, 2:30 p.m.
    ...single colony with a sterile flat toothpick or pipette tip and swirl in a small amount of sterile water...loading dye with glycerol to your samples before pipetting them onto the gel. The figure above summarizes...

  5. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ...replication that can be packaged into replication-incompetent phage particles using a helper system. These ...antimicrobials combines them with both replication-competent phages and antibiotics. Phage therapy fell out...

  6. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    Published
    Sept. 3, 2015, 2:30 p.m.
    ...each other, dressing in costume, and holding competitions (ranging from cook-offs, to corn toss to trivia...enjoyed some friendly -or in some cases, serious-  competition with our corn toss boards, Kan Jam, and Ladder...

  7. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    Published
    Oct. 10, 2023, 1 p.m.
    ...division. These non-plasmid-containing bacteria can compete with and confound the goal of your experiment —...express the Tn5 element, but thankfully, most competent cells do not express this factor. Zeocin is handy...

  8. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ...virions) are not expressed. Note that replication-competent AdV vectors also exist and are used in certain...topic, which is that natural AdVs, replication-competent viruses with a recombinant genome, and adenoviral...

  9. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    Published
    Dec. 7, 2020, 2:53 p.m.
    ...CRISPR-transposons by the Sternberg and Zhang labs (Peters et al., 2017; Klompe et al., 2019; Strecker et ...225 . https://doi.org/10.1038/s41586-019-1323-z Peters JE, Makarova KS, Shmakov S, Koonin EV (2017) Recruitment...

  10. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    Published
    July 30, 2020, 1:15 p.m.
    ...Prashers will find it increasingly difficult to compete (for funding, etc.) with that produced by the confident...the U.S. environment, and a decline in the competitiveness of U.S. science and technology.” This last ...

  12. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    Published
    Sept. 1, 2015, 2:30 p.m.
    ...equipment, categorize all of your minor tools, like pipets, or mini-centrifuges, etc. Be sure to capture as...phones.  As for reagents and consumables (such as pipet tips, glassware, etc), get a simple list from your...

  14. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    Published
    March 28, 2024, 1:15 p.m.
    ...-mimetic sequences that imitate this motif and compete with both small molecule integrin inhibitors and...their targeted integrin subtype, even when in competition with other similar integrins, as reported by ...

  15. March for Science

    Type
    Blog Post
    Published
    April 21, 2017, 2:30 p.m.
    ...References March for Science Website Locke, John, and Peter Laslett. Locke: Two treatises of government student... 15-008: Perspectives on Broader Impacts. The Pipettepen: The March for Science Raises Concerns Over Politicization...

  16. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    Published
    Jan. 4, 2022, 2:15 p.m.
    ...levels of assertiveness and cooperation: avoiding, competing, accommodating, collaborating, and compromising...Though there may be a time and place for avoiding, competing, or accommodating, if you find yourself gravitating...

  17. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    Published
    Aug. 2, 2016, 2:30 p.m.
    ...your primers should be free of palindromic and repetitive sequences, but if present, a minor extension ...template should be isolated from a methylation competent bacterial strain (e.g. DH5α which is dam+). Methylation...

  19. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ...with fluorescent derivatives of its substrate trimpethoprim (TMP-tag) (Miller et al., 2005). Although this...Benzylguanine (BG) Many Easy eDHFR 159 Trimpethoprim  (TMP-tag) Few Easy HaloTag 295 ...

  20. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    Published
    June 8, 2021, 1:15 p.m.
    ...experiences! These applications can be highly competitive, so asking for help from mentors and other students... Imposter: The Effect of Perceived Classroom Competition on the Daily Psychological Experiences of First-Generation...
Showing: 81 - 100 of 219 results