Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering
Type
Blog Post
Published
May 3, 2018, 1:48 p.m.
...similar to previously discovered Cas13 enzymes - what makes these orthologs so special? The first property is...Cas13d enzymes have a median length of ~930aa - making them 17-26% smaller than other Cas13s and a whopping...whopping 33% smaller than Cas9! Their small size makes then easy to package in low-capacity vectors like...broader regulators of Cas13d activity. This property makes WYL proteins an intriguing counterpart to anti-CRISPR... yielded >80% knockdown. It seems that CasRx may make it possible to target essentially any RNA in a cell...and we at Addgene appreciate our depositors for making their tools available to the broader community....