...library comes either with or without mCherry and GFP fluorescent controls. Alternatively, you can order... TF library (aka the MORF Collection) and an accompanying expression atlas, including over 3,500 human...endoderm, and mesoderm). These profiles were then compared to that of reference cell types generated by the...

...be another method to assess transfection success. GFP is often used as a reporter and we will be covering...cells. Of course, these mammalian vectors must be compatible with the cell type they are tranfected into –...called transfection. Transfection is somewhat comparable to bacterial transformation (the introduction...

...the viral protease, Nsp5. Our set also includes a GFP construct as a control. For easier portability, all...the human interactors we found in our map have an impact on virus replication. While we and our collaborators...

...fluorescent protein topics page Get the basics on GFP Find microbiology blog posts Resources on Addgene.org...variant library is monitored by fusing the variants to eGFP followed by an internal ribosomal entry site and...a variant has wild-type levels of abundance, the eGFP will be stable and fluoresce. But if the variant...unstable, it will be degraded, leading to reduced eGFP fluorescence. Cells expressing the library are then...fluorescence-activated cell sorting into four bins based on their eGFP:mCherry ratios. Genomic DNA from each bin are then extracted...and colonization opportunities for bacteria. To compare the artificial leaf to A. thaliana leaf, Bernach...

...via phosphorylation, promoting transcription of a GFP reporter. Subsequent exposure to red light rapidly...expression levels are optimal. These tools are compatible with many strains of E. coli, as well as with...

...ones? Many of the available biosensors use a single GFP or CFP/YFP-derived FRET pair. If you need to free..., a neuron subtype) and specific subcellular compartments for measurements. All of the sensors discussed...calcium concentrations in different subcellular compartments can vary greatly. Additionally, the calcium ...susceptible to artifacts and pH interference, and comparison between experiments can be more difficult due...particularly useful sensor for neuroscientists is CaMPARI. This sensor switches from green fluorescence to... of neurons in vivo to study circuit activity. CaMPARI is available for neuron-specific expression (hSyn...expression (CAG promoter). An improved version named CaMPARI2 is available for neuron-specific expression in...

...non-immunogenic violet-excited GFP variant Vex. To understand the impact of this gene editing tool on the...

...selection cassette. They could also switch from FLAG to GFP or a different epitope. Addgene: Do you have any ...the function of the protein. Myers: We’ve done comparisons of our new system versus standard ChIP-seq for...

...line, Hep3B. From left to right: Mitotracker Red, GFP for the biosensor, Hoechst, and overlay).   ...(DKFZ) who has developed new redox sensitive GFP2 (roGFP2)-based biosensors for live cells. His work focuses..., Pedre Pérez’s biosensor fuses MPST to roGFP2. Because roGFP2 contains two cysteines near the chromophore... anchoring domain), in electroporated in rat hippocampal cultured neurons, imaged 14 days post-transfection...the MPST hydrosulfide can be transferred to the roGFP2 cysteine thiols and change its fluorescence properties... the screen to understand mouse cortical and hippocampal neural microcircuits. “In the longer term, this...

...Expression with Cre-lox Control Protein Activity with GFP Resources at Addgene.org Find KTR reporter plasmids...signal strength, and how long their signals last all impact transduction of the message. To study signaling...dimeric Dronpa is less prone to aggregation in cells compared to the tetrameric Dronpa it was derived from. ...

... for a researcher to come into contact with than GFP. Similarly, if the viral vector carries an shRNA ...materials, you’ll need to use aerosol-tight caps, compatible with your centrifugation speed, to cover the ...

..., Myc and SV40 Nuclear Localization Signals, and GFP. B) Workflow to assess Cas9-PAGE editing with viral...variant has similar photostability and brightness compared to the original, though its maturation is even...cell-specific tropism, with slightly reduced efficacy compared to AA9-X1.1. Importantly, AAV1-X1 is not neutralized...

...Lindsey M. Costantini, and Erik L. Snapp. "Superfolder GFP is fluorescent in oxidizing environments when targeted...with fluorescent proteins (FPs) localized to compartments other than the cytoplasm. That is, unfortunately...investigators realize too late that they have missed the impact of dark, non-fluorescent, and misfolded FP-fusions...quantitative imaging experiments or even negatively impact cells (see Figure 1). When behaving properly, ...positioning of the FP sequence relative to cell compartment targeting sequences, which often have absolute...that FPs are often poorly suited for cellular compartments other than the cytoplasm. Generally, FPs evolved.... Many of the resident proteins within these compartments undergo significant post-translational modifications...

...check out our article: Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection. Cellular...expressed transiently by transfection or stably impacts the success of BiFC experiments. In either case...independent of PPIs. Overexpression can be assessed by comparing the expression levels between BiFC constructs ...localization of BiFC constructs should also be compared against those of their endogenous counterparts...association between FPs independent of PPIs. Simply compare the fluorescence intensity of your BiFC constructs...under relatively physiological conditions when compared to FRET. However, it’s important to note that ...

...contrast, the widely used green fluorescent protein (GFP) gives a bright green fluorescence when exposed to...fluoresce at very low levels. Chromoproteins in nature Compared to the history of fluorescent proteins in molecular...eforRed, asPink, spisPink, scOrange, fwYellow, amilGFP, amajLime, cjBlue, meffBlue, aeBlue, amilCP, tsPurple...

... a superfolder GFP fusion to stabilize the protein in mammalian cells. LwaCas13a-msfGFP can mediate cleavage...the ability to cleave the RNA target.  Table 1: Comparison on Common CRISPR Enzymes Name  Enzymatic Domains...multiplexing; expressing 5 gRNAs simultaneously produces comparable gene knockdown to single gRNA expression. Although... cell stress, and RNA size distribution. When comparing LwaCas13a to shRNA, they found that LwaCas13a ...

...which must be stabilized by monomeric superfolded GFP and averages only ~50% RNA knockdown. In initial ...off-target editing. In one direct transcriptome-wide comparison, REPAIRv2 reduced off-target sites from the 18,385...edits by ~45% and off-target A-to-I edies by ~94% compared to RESCUE.   Addressing the potential use of RESCUE...

...in this case a reporter gene (e.g. luciferase or GFP), regardless of whether it interacts with a transcriptional...transcription. The HIS3 reporter system (e.g. the Gateway-compatible bait Y2H plasmid pEZY202  from Yu-Zhu Zhang’s...particular cell type, or a subset based on a cellular compartment or growth stage). A prey library can either be...

...In cases such as this, an epitope such as c-Myc, GFP or V5 can be used to tag the protein of interest ... need to choose the protein type that is most compatible with the host species and isotype of their primary...developed, which can be used for most antibodies compatible with either Protein A or G.  While commonly used...

...Mammalian Cells Learn about the Dangers of Using GFP for Protein Localization Resources on Addgene.org...line identity and purity making it easier for comparable studies to be performed on a cell line even decades...through working for a variety of pharmaceutical companies including GSK, AstraZeneca-MedImmune, and Infors...