...legal complexities can arise when scientists are sharing their materials. Questions about intellectual property...material transfers. When Addgene entered the plasmid sharing space, we heard over and over again that the legal...were one of the biggest hurdles for scientific sharing. So we set out to streamline this process. We take...material) Affirmation that materials will only be shared with non-profit organizations Assurance that...

... of plasmid sharing  Interested in more facts about Addgene's 10 years of plasmid sharing? Check out this...breakthrough research and your commitment to scientific sharing. In the coming years, we will continue to improve...improve our services and work to make sharing even easier. We can't wait to see what you put on the next ...

...Addgenies Brook Pyhtila and Nicole Waxmonsky Resource sharing shortens the time needed to go from planning an... experiments. Addgene’s dedication to resource sharing has led us to develop a searchable and sortable...submitting their sequences (and plasmids!) so that this shared resource can continue to grow. This post was ...CRISPR Technologies Available Through Resource Sharing. 2016. In press. Additional Resources on the Addgene...

...more about alternative careers for scientists, and share cool science. Unfortunately, it never clicked for...scientists on Twitter: who are they? What do they share? And how they are connected? Here are the highlights...personal on Twitter. Or maybe scientist prefer to share links to research that isn’t behind paywalls. 5....Use Reddit for Science Communication 10 Ways to Share Your Science Why Scientists Should Give Twitter ...

...Video to Share Your Research 5 Reasons to Use Reddit for Science Communication 10 Ways to Share Your Science...struggles in the laboratory routine. We hope that sharing and laughing at these struggles will bring people...like to see portrayed in LEGO form, feel free to share with us at [email protected]. Who knows,...

...manuscript by ZZZ et al., describes ... The authors share a wealth of data on ... The language is ... The ...public on-line repository (like GitHub, Zenodo, Figshare, Triad) while the wet-lab resources, like constructs...passionate about open science, BIG data analysis and sharing whatever she knows with whoever cares to listen...the Addgene blog Find blog posts on scientific sharing Get tips on writing a review article Resources...

...best way to solve problems is to work together and share information broadly. “Otherwise we will all have... come up with discoveries on their own and only share what they think will get the research funded,” she...she learned more about Addgene. “The mission to share critical reagents such as plasmids and viral stocks...He’s also interested to see Addgene expand the sharing platform to other reagents in biology. Luk H. Vandenberghe...

...make the screen-sharing experience more interactive. The button is on the right of the Share Screen control...accessed by anyone at any time. This document can be shared and serve as a springboard for future discussion..., Denfeld BA, de Eyto E, Hampton SE, Keller PS, Sharma S, Lewis ASL, Weyhenmeyer GA, O’Reilly CM, Lofton...

...-C’) that give the feature its characteristic T-shape. The arrangement of the B-B’ and C-C’ palindromic...sequences (A-A’, B-B’, and C-C’ regions) and forms a T-shaped structure. The Rep binding element (RBE) and terminal...different AAV serotypes that vary in their RBE and T-shaped hairpin loop sequences (Wilmott et al. 2019). Most...10.1089/hgtb.2016.133. PMID: 28967288. Shitik, E. M., Shalik, I. K., & Yudkin, D. V. (2023). AAV- based vector...

...years, I've been looking forward to seeing how the sharing of his OSKM factors have affected the stem cell...engineering and stem cells, and the difficulty of sharing banked iPSCs across international borders. While...lost in the details. The speakers at this meeting shared plenty of details, but they also uniformly stepped...ground-breaking work on spinal cord injuries; Jessica Young shared research on stem cells and Alzheimer's Disease;...

...given by Phil Sharp. After a few rotations, Doench decided to complete his PhD work in Sharp’s lab.  Doench... easier for people everywhere…. the nonprofit, sharing ethic that Addgene has behind it is something that... sharing...

... maps and protocols! In reality, there are many shared components across viruses (AAV, lentivirus, etc...This means that lentivirus and gamma-retrovirus share a lot of core components, so we will discuss them...are slightly different in sequence. Both LTRs do share common repeat regions in addition to their unique...components Lentiviruses have gone through their fair share of lab evolution. There are 1st, 2nd, and 3rd generation...

...Scientific Writing.” In this post, we’d like to share parts of our workshop with you!  Accessibility  ...your readers are now a team, working together to share information. Sometimes it is most helpful to use...practice image. We know; we asked each group to share with the rest of the workshop.  For Figure 1, Addgene... will flow out the bottom syringe."Feel free to share your alt text in the comments! We learned a lot ...

...educational content Addgene truly believes that sharing speeds science, and our entire mission is to enable...informative content to help researchers in creating and sharing knowledge, related to the types of materials we...originally started as a way to easily store and share in-depth answers to frequently asked questions. ...licensed and cited figures from papers or image sharing repositories. Then we format, do a final review...

... connexin localized correctly to gap junctions (Shaner et al, 2008). Obligate dimers (such as dTomato)...assay to demonstrate monomeric behaviour in cells (Shaner et al, 2013; Bindels et al, 2017). Still, the data...knowledge and experience that is gathered can be shared by publishing it as (part of) figure 1, uploading...or cells or organisms that are engineered can be shared to simplify the comparison by others. Together,...

...proteins may display reversible intensity changes (Shaner et al, 2008; Bindels et al, 2017) and photoswitching...use widely varying amounts of excitation power (Shaner et al, 2008). In confocal laser scanning microscopy... concentration may affect photobleaching rates (Shaner et al, 2008). This brings us to the key question...does not follow a simple mono-exponential decay (Shaner et al, 2008; Bindels et al, 2017) and cannot be...

...scientists. You can find him on Twitter @krisshamloo and at krisshamloo.com     Additional Resources on the Addgene...for them and let them in on the fun too. Kris Shamloo is a Software Engineer at Addgene and is interested... Kris Shamloo...

...also don’t have to go back to school right away. Shannon Rinaldi, Addgene’s Lab Manager, was taking classes...stay in science.” Addgenies Beth Kenkel and Shannon Rinaldi in the Addgene lab! Read their Instagram... publish their research and are not so keen on sharing their results in fear of getting scooped, she said...side of science, she stuck with it. Lab Manager Shannon Rinaldi values loving what she does. Her parents...

...Interactions. Filonov GS, Verkhusha VV. Chem Biol. 2013 Vladislav Verkhusha iRFP GAF domains from miRFP709...Shcherbakova et al. Nat Commun. 2016 Vladislav Verkhusha Multiple FAST Fluorescence-activating and absorption...of protein-protein interactions in living cells. Shao S, Zhang H, Zeng Y, Li Y, Sun C, Sun Y. Commun Biol...

...can optimize your growth time, temperature, and shaking speed to maximize cell density and plasmid yield... total culture volume and you should ensure the shaker speed is fast enough for sufficient gas exchange...culture. Generally, large cultures in flasks may be shaken around 220 RPM, but smaller cultures, especially...especially those in deep-well plates, require a faster shaking speed for proper aeration (260-300 RPM). 37℃ doesn...