...References Cele S, Gazy I, Jackson L, Hwa S-H, Tegally H, Lustig G, Giandhari J, Pillay S, Wilkinson E, ...

...Genesis 34, 1–15 (2002).PubMed PMID: 12324939. 3. S. E. McGuire, G. Roman, R. L. Davis, Gene expression... PMID: 21356876. 5. J. Chow, Z. Marka, I. Bartos, S. Marka, J. C. Kagan, Environmental Stress Causes Lethal...PMID: 22205518. PubMed Central PMCID: PMC3574576. 7. S.-L. Lai, T. Lee, Genetic mosaic with dual binary transcriptional...

...861-72. PubMed PMID: 18035408. 3. Takahashi, K. and S. Yamanaka, Induction of pluripotent stem cells from...temperature-sensitive Sendai virus vectors. Proc Natl Acad Sci U S A, 2011. 108(34): p. 14234-9. PubMed PMID: 21821793... of canonical Wnt signaling. Proc Natl Acad Sci U S A, 2012. 109(27): p. E1848-57. PubMed PMID: 22645348...10): p. 1836-46. PubMed PMID: 22976355. 34. Wang, S., et al., Differentiation of human induced pluripotent...p. 82-94. PubMed PMID: 23329641. 36. Jopling, C., S. Boue, and J.C. Izpisua Belmonte, Dedifferentiation...): p. 987-1000. PubMed PMID: 3690668. 40. Ferber, S., et al., Pancreatic and duodenal homeobox gene 1 ...

...C->G or C->A conversions that can happen with BE3’s. T These byproducts likely resulted from excision ...NM, Barrera LA, Born DA, Edwards A, Gehrke JM, Lee S-J, Liquori AJ, Murray R, Packer MS, Rinaldi C, Slaymaker... Grünewald J, Zhou R, Lareau CA, Garcia SP, Iyer S, Miller BR, Langner LM, Hsu JY, Aryee MJ, Joung JK...at Addgene.  Nishida K, Arazoe T, Yachie N, Banno S, Kakimoto M, Tabata M, Mochizuki M, Miyabe A, Araki... X, Zhu B, Chen L, Xie L, Yu W, Wang Y, Li L, Yin S, Yang L, Hu H, Han H, Li Y, Wang L, Chen G, Ma X, ..., Geng H, Huang W, Pang X, Yang Z, Wu Y, Siwko S, Kurita R, Nakamura Y, Yang L, Liu M, Li D (2020) Dual...

...PubMed Central PMCID: PMC3193421. 4. Hacein-Bey-Abina, S., et al., LMO2-associated clonal T cell proliferation...5644): p. 415-9. PubMed PMID: 14564000. 5. Seki, T., S. Yuasa, and K. Fukuda, Generation of induced pluripotent...(4): p. 718-28. PubMed PMID: 22422317. 6. Diecke, S., et al., Recent technological updates and clinical...transformation-deficient Myc. Proc Natl Acad Sci U S A, 2010. 107(32): p. 14152-7. PubMed PMID: 20660764...

...Powers S, Miranda C, Dennys-Rivers C, Huffenberger A, Braun L, Rinaldi F, Wein N, Meyer KC, Solano S, Nguyen....cell.2013.01.038 Guy J, Gan J, Selfridge J, Cobb S, Bird A (2007) Reversal of Neurological Defects in...Sinnamon JR, Kim SY, Fisk JR, Song Z, Nakai H, Jeng S, McWeeney SK, Mandel G (2020) In Vivo Repair of a ...

.... Many thanks to our guest blogger, Jess S. Boehm. Jesse S. Boehm is the Associate Director of the Cancer... This post was contributed  by Jesse S. Boehm, the Associate Director of the Cancer Program at the Broad...

...Konermann explained, was new knowledge about Cas9’s crystal structure. “I thought maybe we could use information...target DNA. Nishimasu H, Ran FA, Hsu PD, Konermann S, Shehata SI, Dohmae N, Ishitani R, Zhang F, Nureki...activation by an engineered CRISPR-Cas9 complex. Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena...

...submit published or unpublished plasmid(s) online or submit plasmid(s) using a spreadsheet. Fields marked ...following descriptions for your plasmid: Encodes insert(s) A plasmid with one or more genes, protein coding...if you’d like to distribute your bacterial strain(s) through Addgene. Description – please indicate the...Figure 6: Enter information about the gene/insert(s). Step 3: Cloning Information Please enter the name...

...Natl Acad Sci U S A. 1991;88(20):8972-6. Moks T, Abrahrnsen L, Oesterlof B, Josephson S, Oestling M, Enfors...polyhistidine tag, alternative fusion tags were used, such as S. aureus’ Protein A (Moks 1987). These tags, however...

...10.1016/j.neuron.2016.01.040 Takeda S, Kadowaki S, Haga T, Takaesu H, Mitaku S (2002) Identification of G protein-coupled...

... to dramatically alter levels of expression. Cas9’s ability to alternately serve both as a means of modifying...gRNAs An alternative approach is to modulate Cas9’s nuclease activity by modifying the gRNA with which...nucleotides of complementarity to a target site, Cas9’s DNA binding capability remains intact while its nucleolytic...

...compared head-to-head with the E. coli and BioID2 BirA*s in the context of RaPID for their ability to detect...proteins were then subtracted from Ramanathan et al.’s RaPID datasets. Applications of RaPID RaPID sounds..., Rao, D.S., Neela, P.H., Zarnegar, B.J., Mondal, S., Roth, J.G., Gai, H., Kovalski, J.R., Siprashvili...

... for at least 4 hours.   If your final RNA sample(s) are resuspended in water or TE buffer, store them...break down RNA upon cell lysis.  In the late 1970’s, Chirgwin and colleagues showed that a strong protein...extraction. Researchers at the NIH in the mid 1980’s set out to develop a protocol that skipped the ultracentrifugation...

...References Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S. A modular cloning system for standardized...transcribed together. They can express one or more gene(s), and typically begin with a promoter and end with...

...technology and how It works 18:02 - 19:56 : Why Sample 6's first test was for Lysteria 19:56 - 24:41 : Who is.... Koeris' Website, biotechstart.org Read Sample 6's Paper on Bacteriophage-based Diagnostics Additional...

...plant cells. Like other model systems, expression of S. pyogenes Cas9 or Cas9 variants (hereafter referred.... References Belhaj K, Chaparro-Garcia A, Kamoun S, Nekrasov V (2013) Plant genome editing made easy:...https://doi.org/10.1128/mmbr.67.1.16-37.2003 Khatodia S, Bhatotia K, Passricha N, Khurana SMP, Tuteja N (2016...7: . https://doi.org/10.3389/fpls.2016.00506 Kwak S-Y, Lew TTS, Sweeney CJ, Koman VB, Wong MH, Bohmert-Tatarev...

....  Mahlandt, E. K., Palacios Martínez, S., Arts, J. J., Tol, S., van Buul, J. D., & Goedhart, J. (2023...single-component systems take advantage of BcLOV4’s light-induced binding of the plasma membrane. In previous...doi.org/10.1016/j.ceb.2015.08.005.  Tei, R., Bagde, S. R., Fromme, J. C., & Baskin, J. M. (2023). Activity-based...

...Trinklein ND, Thie H, Wilkinson IC, Tandon AK, Anderson S, Bladen CL, Jones B, Aldred SF, Bestagno M, Burrone... XY, Eng H-Y, Lane D, Lefranc M-P, Clark M, Dübel S (2018) When monoclonal antibodies are not monospecific...Publications, New York. Helma J, Cardoso MC, Muyldermans S, Leonhardt H (2015) Nanobodies and recombinant binders...

...experiments: Retrieve Ensembl Transcript ID(s) of your favourite gene(s) from Ensembl genome browser: https:/...index.html Design crRNA/ssODN: Using Ensembl Transcript ID(s) as an input query, pick crRNA (guide RNA sequence...Garcia-Diaz C, Blin C, Grant V, Bulstrode H, Gogolok S et al. 2017. Efficient CRISPR/Cas9-assisted gene targeting...PubMed PMID: 9928493. 4. Liang X, Potter J, Kumar S, Ravinder N, Chesnut JD. 2017. Enhanced CRISPR/Cas9...