...of Nextflow supporters existed who had created a shared resource of pipelines for genomic data. Thanks ...safely operates in isolation, and can be easily shared with others without any complicated setup. We created...needed a user interface. In our next post, we’ll share our story about how we used Nextflow Tower to augment...

...Resource Center for Rett Syndrome research. With a shared passion for facilitating research and discovery...Novartis and rebranded to Novartis Gene Therapies, and Taysha Gene Therapies, founded on Gray’s work, are each...their MECP2-related plasmid tools with Addgene and share additional resources. Plasmid tools to facilitate...

...by researchers around the globe. These organisms share many genes with humans, are easily maintained in...for the reasons above but because they actually share many biological properties and processes with our...gradually become an important model organism. They share about 70% of their genes with humans and 85% of ...

...some aspects of their design.  Protein size and shape matters The green fluorescent protein (GFP) from...connexin) has been used as a proxy for dimerization (Shaner et al., 2008 - supplementary figure C). However...PMID: 15247428. PubMed Central PMCID: PMC490022. Shaner, Nathan C., et al. "Improving the photostability...

...(DFHO), a fluorophore modified from DsRed. DFHO shares many structural properties with DFHBI, but its ... Orange Broccoli are derived from Broccoli, and share similar properties to Broccoli despite binding to...PubMed Central PMCID: PMC4923196. Panchapakesan, Shanker Shyam S., et al. "Ribonucleoprotein purification...

...solution for which they are sure that there will be no sharp changes in costs once they establish the system ...functionalities include comments, notifications, sharing files, assigning team members to certain tasks,...collaborating with other labs or clients, you might need share data with them including reports and protocols. ...

...applications, including IF (or IHC or ICC), and will share some of their results and corresponding protocols...may also have tested an antibody in IF and will share their findings either through their publications...poorly in a given context, let your community know! Sharing your experience, positive or negative, will help...

...between protein abundance and band intensity is an S-shaped curve. Sparse proteins may not be detected at all...Created with BioRender.com. To see why this S-shaped relationship poses problems for proteins of very...this gel is misleading. We can see why on the S-shaped curve. On the protein abundance axis, both blue...

...FSC-W/SSC-W, respectively. H, A, and W describe the shape of a histogram, which graphically represents the...y-axis from SSC to FSC can alter the positioning and shape of the populations displayed. As the separation ...Heisig is a Research Associate in the lab of Arlene Sharpe at Harvard Medical School. His projects include...

...specialized equipment, try finding institutes that offer shared equipment that you can use at a lower cost. Don't...value in the process of creating a budget; you can share your budget with fellow researchers and save them...future. Showing that you are willing to work and share with others will help prove that you will be an ...

...recombineering can be found in Thomason et al and Sharan et al (a few strains can also be found at Addgene... you used the lambda red recombineering system? Share your tips below! Many thanks to our guest blogger...doi:10.1128/ecosalplus. PubMed PMID: 27223821. 4. Sharan SK, Thomason LC, Kuznetsov SG, Court DL. Recombineering...

...chromosomal integration of larger expression cassettes (Shaw et al., 2023). Those looking to express high levels... we would love for you to deposit it with us to share it with the scientific community! Good luck and ...86. Crossref, https://doi.org/10.1021/sb500366v. Shaw, William M., et al. “A Multiplex MoClo Toolkit for...

...specific epitope recognized by either antibody was not shared. Tables describe that the antibodies both recognized...on different primary blood leukocytes. The file shared also contains information about the labeling pattern...References Ayoubi, R., Ryan, J., Biddle, M. S., Alshafie, W., Fotouhi, M., Bolivar, S. G., Ruiz Moleon,...

...Quick Tip series on YouTube! In this series, we share quick tips to help you master fundamental technical...  Want your quick tips even quicker? We’ll be sharing our favorite parts of every quick tip video on ...

...Data Hub Challenge, now taking place Dec. 6, 2022! Share your AAV data, connect with other researchers working...t-shirt.  Our judges, Drs. Connie Cepko, Ian Wickersham,  and Dr. Bobae An will host a Q&A session after...

... of today, will stop posting, monitoring, and resharing content on X.  Addgene deeply values our community...knowledge, conversations, and community created and shared on our feed, we will not delete @addgene on X. ...

...scientific community. Data on CRISPR and synBio plasmid sharing In a recent review on CRISPR technology, for example... over time. You can see these data in the slide share. Addgenies are proud of the role we are playing...

... post. Alongside our efforts to facilitate the sharing of reagents and data, we’re also publishing research...supplementation to increase AAV2 yields and we’re eager to share more! Lastly, we’ll be at SfN2019. If you’re attending...

... time even as that lab bench is calling, let me share my top five new favorite science comic sites with... science comic not on our list? Let us know by sharing it with us in the comments.   ...

... expanding gateway compatible vectors currently shared amongst the zebrafish community. Don E, et al.... both agarose and polyacrylamide gels. They are shared through Addgene without licensing restrictions.... hope to see many more of their useful reagents shared via Addgene. Narayan S, et al. Cell Reports. 2017...

...Lab Contest! Though 2021 had more than its fair share of challenges, the annual Deck the Lab Contest brought... post on Instagram                     A post shared by Xi Chen (@seleena78)   Honorable Mention: Culture...

...years ago we used the #12DaysOfCRIPSR hashtag to share new CRISPR content designed to help you with your...the #12DaysOfCRISPR is back on Twitter where we shared one of these updated blog posts each day. If you...

..., we love to give scientists the opportunity to share their knowledge. One of our goals is to be a go-...to reach out to you, our readers, and ask you to share your expertise through our blog. Do you have skill...

... of science communication, tips and tricks for sharing your science with others, and introducing science...Addgene TikTok at the end of this month! We’ll be sharing our educational resources and tips and tricks for...

...science-themed submissions: string lights hung up in the shape of RNA, light-up neurons on the lab door, and lot...cardiovascularresearch #parkinsonsdisease A post shared by Ladies in labcoats (@ladiesinlabcoats) on Dec...

... 2013 to connect with the scientific community, share scientific discoveries and developments, and provide...neuroscience), or explore other areas such as scientific sharing or publishing, under the Other dropdown menu. You...

...excited to have more and more scientists offering to share their stories – about their research, their job ...are always looking for guest bloggers who want to share their research with the community, so email us at...

...bacteria on the toothpick was very cool!” and Izzy Shaw, who was excited about “Seeing a real lab!” And ...over the world know that we can help with plasmid sharing! Judging by how many prizes were given out during...

...publications alone aren’t enough. Scientists are sharing what they call “raw science” – data sets, code,... Mendeley are allowing researchers to track and share their own libraries and offering another way to ...

... of sharing science and making access to materials easier is improving the tools you use to share and ...

...PC) Chromas (PC) 2. You should see individual, sharp and evenly spaced peaks …like these…. 3. Expect...for originally publishing this and allowing us to share it with our readers! The chromatograms in this article...

...featuring plasmid tools from depositors who quickly shared their reagents: detection methods, synthetic nanobodies...19 pandemic, we saw the need to expand reagent sharing even broader and began working with tech transfer...

...connections is your university’s alumni database. Sharing a school is a little connection that many people... all kinds of people to connect with you and to share their stories. Thanks to the Benjamin Franklin Effect...

...an antibiotic resistant bacterial infection, she shared that tweet with Jan Zheng, a UX designer that she... open science Find blog posts about scientific sharing Find blog posts about networking Additional resources...

...Jul 2;8(7):e67902. Bähler J, Wu JQ, Longtine MS, Shah NG, McKenzie A 3rd, Steever AB, Wach A, Philippsen...943-51. Longtine MS, McKenzie A 3rd, Demarini DJ, Shah NG, Wach A, Brachat A, Philippsen P, Pringle JR....

... life on Earth. Selective breeding methods have shaped the plants that we grow and eat, and genetic engineering...References Sahoo, Dipak Kumar, Nrisingha Dey, and Indu Bhushan Maiti. "pSiM24 is a novel versatile gene expression...

...the strong, cross-disciplinary collaboration they shared to the work they did together. They especially ...making their Skype communications extra fun) who shared first authorship on their seminal, cooperative ...

....   Advice for success in academia How can we shape our time in academia to maximize productivity and...success.” 2. “Enjoy Teamwork.” Be friendly, open, sharing, rather than intensely competitive. Be curious ...

...which let you lock and hide individual vectors (shapes defined by 2D points connected by lines and curves...wide selection of tools for drawing and editing shapes and like Vectr, Inkscape uses vector graphics. ...

...about practices I could bring both to Addgene, and share with my peers. In fact, there was so much useful...newsletter Help other scientists save resources by sharing plasmids Find plasmids at Addgene ...