...T. Expression of recombinant antibodies. Front Immunol. 2013 Jul 29;4:217. doi: 10.3389/fimmu.2013.00217...the production of recombinant Erythropoietin. J Biotechnol. 2020 Feb 10;309:44-52. doi: 10.1016/j.jbiotec...beads from very high cell density suspension. Biotechnol Bioeng. 2021 Sep;118(9):3499-3510. doi: 10.1002...

...metabolic cassettes (Zhang et al. 2020); however, the technology potential enabled by CRISPR-transposons has yet...CRISPR biology and CRISPR genome engineering technologies. Message or follow him on twitter @Vopinator...multiplexed bacterial genome engineering. Nat Biotechnol. https://doi.org/10.1038/s41587-020-00745-y Zhang...

...approximately 20,000 droplets using water-oil emulsion technology. Each droplet contains the ingredients for amplification... added benefit, replicates are built into the technology. One well (containing thousands of droplets) ...titration, there are many applications for the technology.  Droplet digital PCR is well suited for the ...

... CRISPR–Cpf1 Using a Single crRNA Array." Nat Biotechnol. 35.1 (2016): 31-34. PubMed PMID: 27918548. ...specificities of Cpf1 endonucleases in human cells. Nat Biotechnol. (2016). PubMed PMID: 27272384 Find plasmids...CRISPR-Cas Cpf1 nucleases in human cells." Nat Biotechnol. (2016). PubMed PMID: 27347757. Find plasmids...

...editing in mammalian cells and embryos. Nature Biotechnology, 42(4), 638–650. https://doi.org/10.1038/s41587...with an N-methylpurine DNA glycosylase. Nature Biotechnology, 41(8), 1080–1084. https://doi.org/10.1038/s41587...and C-to-G editing in mammalian cells. Nature Biotechnology, 42(10), 1538–1547. https://doi.org/10.1038/...

... able to distribute materials containing FLEx technology. Read the genOway press release for more information...Recombinase Evolved by Cycling Mutagenesis.” Nature biotechnology 16(7): 657–62. PubMed PMID: 9661200.  3. Cardin...at the Cellular Level in the Mouse.” Nature Biotechnology 21(5): 562–65. PubMed PMID: 12665802. 9. Villa...

...showed APEX2 can use an orthogonal substrate, alkyne-phenol, to label proteins with an alkyne group for click... tapes for intracellular recordings. Nature Biotechnology, 41(5), 631–639. doi: 10.1038/s41587-022-01524...monoclonal antibodies for enhanced multiplex immunolabeling of brain. eLife, 8, e43322. doi: 10.7554/eLife...

...) either produced de novo using yeast display technology or produced from hybridoma antibodies developed...synthetic rAbs, developed using yeast display technology, are directed to integrins that bind the Arginine-Glycine-Aspartate...science, IPI hosts events and training courses on technologies and laboratory techniques related to antibody...

...impact (15). Diversity and inclusion in science, technology, engineering and mathematics (STEM) (17). This... 27). In order to achieve the breakthroughs, technologies, and treatments promised by modern research,...Trump’s first budget Environmental Science and Technology: Crossing The Imaginary Line The New York Times...

...avidin-biotin technology to improve cell adhesion on nanofibrous matrices.” Journal of Nanobiotechnology 13.1 ...interaction with avidin for molecular biology and biotechnology purposes.  Once a polypeptide is biotinylated...

...Multiplexing and Optogenetics across Scales. Trends Biotechnol 36, 1230-1243 (2018). Shcherbakova, D.M. et al...encoded calcium indicator for in vivo imaging. Nat Biotechnol 39, 368-377 (2021). Shcherbakova, D.M. Near-infrared... fluorescent protein for in vivo imaging. Nat Biotechnol 29, 757-761 (2011).   ...

...especially in in vivo models, and new Cre-lox based technologies are still being developed today. In this post...name Brainbow is truly fitting for this colorful technology. Optimizing the system: Brainbow-3 Although Brainbow... et al. released a refinement of the Brainbow technology, Brainbow-3.0, with the goal of overcoming the...

...They also have plans to expand to other protein technologies later. Organizations such as BenchSci and FPbase...fluorescent proteins so you can best apply these technologies to your own research needs. Other companies ...series of blog posts on the defunct genome editing technology NgAgo). In addition, there are organizations ...

...recombination mediated by 28 bp direct repeats. Mol Biotechnol. 2008 Nov;40(3):252-60. doi: 10.1007/s12033-008... on efficacy, safety and upstream production. Biotechnol Adv. 2009 Jul-Aug;27(4):353-70. doi: 10.1016/...seed-stock and fed-batch fermentation processes. Biotechnol Bioeng. 2009 Aug 15;103(6):1129-43. doi: 10.1002...

... Why might one be interested in state machine technology? Biological research has produced a massive amount...PBAD), and the A118 gene downstream of the diacetylphloroglucinol (DAPG)-inducible promoter (PPhlF).  Output...synthetic circuits from genetic context." Nature biotechnology 30.11 (2012): 1137-1142. PubMed PMID: 23034349...

...tubes, snap freeze in liquid nitrogen or a dry ice/ethanol bath, and store at -80° C. Once thawed, the AAV...purposes. The University of Pennsylvania and the biotechnology firm Biogen have recently collaborated to develop...is their ability to be used with new exciting technologies (such as CRISPR/Cas9) as they allow for gene...

.... With the widespread adoption of CRISPR/Cas9 technology and rapid improvements in the field, it has recently...experiments (in collaboration with Integrated DNA Technologies, USA). These synthetic RNAs can also be purchased...delivery of gRNA, Cas9 nuclease, and donor DNA. J Biotechnol 241: 136-146. PubMed PMID: 27845164. 5. Clarke...

..., which are pivotal in their application in biotechnology (Grimm & Lavis, 2022). These variations directly...toxicity due to the permeation process. Advanced technologies in fluorescent dyes/ligands Throughout the history...capabilities of fluorescence-based techniques in biotechnology. Recent examples of advancements include the...

... variety of fields including neuroscience and immunology. They can be used for qualitative and quantitative...Historical Perspective and Future Outlook. Front Immunol 8: . https://doi.org/10.3389/fimmu.2017.01589 ..., IMMUNOGENS, VACCINES, AND IMMUNIZATION. In: Immunology Guidebook. Elsevier, pp 17–45. https://doi.org...

...the history of neuroscience research we have a technology that allows us to show causality in neural circuits...technique doesn’t have its limitations or that other technologies need to be discarded but clearly we are in an...and relatively cheap (compared to many other technologies and techniques in the sciences). But how exactly...

...human endeavor including but not limited to biotechnology, chemistry, architecture, engineering, mathematics... sector are transferrable to industry such as technology transfer, communications, medical writing, compliance...Casseus Law. Driven by her own love of science and technology, Rachel is proud to assist companies and foreign...

...permeabilization protocol, but very poorly in a methanol fixation-permeabilization protocol. Although the...targets, we usually test 3 different fix/perms (methanol, PFA-saponin, PFA-triton). The fix/perm solutions....1% saponin; 4% PFA and 0.1% Triton X-100; and methanol, we found that the optimal perm/fix solution depended...

...share and describe some incredible new CRISPR technologies developed and widely adopted in the past few...guide for a great introduction to what CRISPR technologies have to offer. Happy CRISPR-ing! More resources...

...nervous system With the advent of viral vector technology, new methods have been developed to study neuronal... Gradinaru Lab at the California Institute of Technology generated novel AAVs (Chan et al., 2017) that...targets neonatal neurons and adult astrocytes. Nat Biotechnol 27, 59–65. PubMed PMID: 19098898. PubMed Central...

...are not with denaturing agents like DTT or β-mercaptoethanol.  Denaturing proteins To denature your proteins...you'll want to use SDS and either DTT or β-mercaptoethanol (BME). DTT is a stronger reducing agent than...without staining; simply wet your membrane with 20% methanol and place it on a light box after drying. Nitrocellulose...

... focuses on producing biotechnologies in the areas of genome editing, immunology, and gene therapy.   ...

...Plasmid technologies are constantly evolving, but sometimes a technology is so useful it forever enhances...

...podcast that introduces you to 4 new plasmid technologies from one of our recent newsletters. Listen ...you enjoy this new way to learn about plasmid technologies. If you have any comments about the Hot Plasmids...

...views) was an update on new CRISPR genome editing technology, Cpf1. Written by our Science Communications ...the end of the post also really helped put this technology into a concrete context. I recommend this post...

... for more detailed information about this new technology. Our Plasmids 101 series continues to grab scientists...questions or if you have a topic, researcher, or technology you would like us to feature in the coming year...