...IF-ICC.) This method was chosen because cells transfected with a plasmid to express the target antigen ...

...Animal Models Q9: Is there any difference in transfection efficiency when delivering these viruses via...

...cells after the plasmids have been introduced by transfection or electroporation. All you do to create a protein...

...plasmid electroporation (episomal) [7, 8] and mRNA transfection [9]. Many of the plasmids used for these methods...Weintraub, and A.B. Lassar, Expression of a single transfected cDNA converts fibroblasts to myoblasts. Cell,...

...of cell types that are typically ‘difficult to transfect’, such as primary cells. They can also be directly...

...When a dish of mammalian cells is transiently transfected with a plasmid containing a fluorescent protein...

...PubMed PMID: 26914317. 4. Mock, U. et al. mRNA transfection of a novel TAL effector nuclease (TALEN) facilitates...

...base editor is split into two plasmids and co-transfected into cells for reconstitution. Image from Levy...

...an unregulated fashion and thus a marker for transfection. The other (mKate2) is regulated in the 3’ UTR...

...PPI) studies in plants that rely on transient transfection systems using Agrobacterium (such as Nicotiana...

...pSpCas9(BB)). The 2A-GFP allows for screening of transfected cells. Find pSpCas9(BB)-2A-GFP (PX458). lentiCRISPR...

...laboratories. The development of the transient transfection platform for assaying transcriptional activity...

...single gene. The gRNA expressing cells were then transfected with a Cas9-expressing plasmid to generate a ...luciferase. The bait and prey plasmids are co-transfected and proteins are immunoprecipitated from the ... plasmid is also available). Second, they co-transfected short-arm donor vectors containing mAID and either...efficiently creating stable cell lines using direct transfection precluding the need for virus. Furthermore, ...

... plasmid). Viral production then proceeds by transfection of all three plasmids into a producer cell line...

...targeting experiments allows you to visualize transfection efficiency and even track localization in cellular...

...bonds, monobodies can be easily expressed in transfected eukaryotic cells, allowing for them to be used...

...hippocampal cultured neurons, imaged 14 days post-transfection, excited at 505 nm (right). Scalebar represents...

... include both scrambled RNA motifs and cells transfected with BirA* protein alone (i.e. no RNA bait). ...

...Transformation, Transduction, Bacterial Conjugation, and Transfection Resources on Addgene.org Addgene's Synthetic...

...over for other applications, like PCR, cloning, transfection, or long-term storage. But what about those ...