...plagued by a steady barrage of problems. Why didn’t my PCR work? Why didn’t my ligation work? Why are there ... being able to figure out what went wrong with a PCR is a pretty niche skill; outside of a lab, where ...

... once decided that to teach high school students PCR, they first had to understand hydrogen bonds and ...unlikely to be able to follow a YouTube video on PCR, while a senior lab scientist is likely to be quickly...

...perform PCR against components of the viral vector using the harvested viral solution as a PCR template...

...developed by detecting these chimeric DNA fragments by PCR or next-generation sequencing. Figure 1: Overview...reversed so that DNA fragments can be detected by PCR or deep sequencing. A drawback of the original...

... Weigel and Glazebrook, 2002. Tips: Do a colony PCR on your agrobacterium: Getting a transformant takes...remaining starter culture can be used for colony PCR and glycerol stocks. Dip/drip plants more than once...

...presence of pathogens in environmental samples via PCR. We hope these plasmids will help Dr. Duedu inspire...

... use detector cell lines, ELISA, fluorometry, or PCR-based approaches to detect even low levels of contamination...detection, Uphoff et. al. outline a do it yourself PCR-based approach complete with freely available positive...

...facility requires you to perform your own Big Dye PCR amplification reaction (as opposed to using the all...

...Reaction (PCR) by using a biotinylated primer. Biotin can also be enzymatically incorporated during PCR using...

...differentiation process. Moreover, a splinkerette-PCR assay may be used to map the specific genetic loci...and D. J. Adams, “A high-throughput splinkerette-PCR method for the isolation and sequencing of retroviral...

...degrade the double helix, fragmenting plasmid DNA, PCR product, or chromosome. One of the main causes of...

...procedure daily before the advent of techniques like PCR and DNA synthesis. Most molecular biologists bought...

...single-stranded DNA sequence used as an initiation point for PCR amplification or sequencing. Primers can be exploited...

... your cloning experiments and tired of having to PCR many colonies to find a successful clone? Addgenie...

...Mellor, Founder and CEO of seqWell Inc. Plasmids and PCR products are the bread and butter of molecular biology...

...This method is best used for DNA fragments (like a PCR product). This method also gives an indicator of ...

...remove the selection marker. Other labs have used PCR-based screening to detect mutants, but this method...

...(nCas9). Similar to mutagenesis with error prone PCR, EvolvR uses an error prone polymerase to introduce...

...the crosslinking. Finally, purify your DNA with a PCR purification kit or with a phenol-chloroform extraction...through a variety of downstream methods, such as qPCR or next-gen sequencing. ChIP is an incredibly useful...isolated and purified DNA, such as sequencing or qPCR. Yay!  Where’s the challenge in that?  So while ...

...down candidates for more specific analysis, like PCR or restriction digest. Make sure you use a proper...