... next steps in the process involve repairing chromosomal DSBs. It is important to know that cells possess...extensive DNA synthesis. NHEJ also finishes repair of most types of breaks in tens of minutes – an order of...should possess indels in the majority of their chromosomes within a day. Another factor expected to impact...or more can simultaneously introduce a pair of chromosome breaks, and could result in deletion of the intervening...Stark JM (2009) Limiting the Persistence of a Chromosome Break Diminishes Its Mutagenic Potential. PLoS...EA, Giovannangeli C, Jasin M, Brunet E (2014) Chromosomal Translocations in Human Cells Are Generated by...

...Harvard Medical School working on human artificial chromosomes. Montreal, QC, Canada 45.5017°N 73.5673°W – Vanessa...to full reproductive health care. And it’s needed most in low-income and minority communities, where these... hardest. According to the Guttmacher Institute, most women of reproductive age live in a state with at...about my work because it's important to our health.  Most people won't argue with that idea, but they might...into a successful PhD thesis. We use the same atmospheric physics to understand exoplanets that we use ...organized the march was, as well as the friendly atmosphere that was created by the many thousands of science...science, but I worried (and continue to worry) that most who participated will consider that marching in ...

...Microscopy Fluorescent Widefield Microscopy is the most common and simplest form of fluorescent microscopy...developed that allow for sub-diffraction limit imaging. Most often, these techniques provide a 2-10x improvement...interference from blurry out-of-focus light. The most difficult characteristic to capture in these types...resolution on the millisecond scale may be needed. Most commonly these experiments are performed on a fluorescent... a fast, sensitive detector such as a scientific CMOS camera. If plasma membrane dynamics are the main...small model embryos >15 μm Thsee are some of the most challenging samples to image as they require both...quality images for this type of sample as they are most efficient at excluding out of focus light. However...

...stream to commute to and invade multiple organs, and most importantly escape the immune mechanisms of the ...known as the tumor microenvironment - a vital atmosphere created by the cancer cells to shield themselves...cell-surface tolerance-producing proteins are one of the most commonly used immunotherapeutic agents. Currently...receptors (CARs) on their surfaces have been the most acclaimed form of immunotherapy in recent times.... validation to identify populations that are the most likely to be positively impacted by the trial. During...potential beneficiaries. Collaborative therapy For the most efficient outcomes, collaborative therapies must...questions in this exciting field of research. One of the most important: How will cancers evolve and mutate based...

...efficiency than in iPSCs, but with the caveat of mosaicism. To circumvent this problem, they co-injected ...Indeed, they observed that 41/42 embryos were non-mosaic at 3 days post fertilization. Since Ma et al. designed...term ‘editing.’ While repair using endogenous chromosomal DNA is appealing for heterozygous mutations, ...needed to define whether off-target editing or mosaicism would occur. Although Ma et al. looked at various...this detailed analysis is not possible with IVF, as most of the cells are needed for proper development. .... Ma et al.’s experiments showed a 3% mosaic rate, and this rate would need to be much, much lower before...

...email me with suggestions.  Most coaches have websites you can review and most create useful online content...and do not know where to look for a coach. Many (most?) graduate students and postdocs may feel they have... have limited funds to invest in this. However most coaches who focus on scientists are sensitive to this...effective and also help keep the coaching costs low. Most will work with you remotely (by phone, Skype or .... This larger shop might appeal to some although most scientist coaches can help with transitioning out...

... be the most common poster size, but this can vary as well.  Sections of your poster In almost all scientific...be as concise as possible, communicating only the most important results to your audience. With a poster...Adobe also offers free poster templates, although most are vertical.  Large central box. Another commonly... of this style is that it will display your data most prominently, making it the star of your presentation...navigating the poster session Find tips to make the most of your conference attendance Read all of our blog...

...fast-tracked development of diagnostics. Mostly transmitted by Aedes mosquitoes (aegypti and albopictus) and, aided...virus had received little attention and, for the most part, had been associated with low morbidity and...conducive to the spread of the virus by the Aedes mosquito (Messina et al, 2016). We quickly assembled a ...true team effort and many late nights, not unlike most research, but the urgent need for the test did catalyze...community when we needed additional expertise. Perhaps most striking is that there were seven institutions involved...

...preparation and day-of game plan will ensure you have the most beneficial scientific conference experience possible...your poster as a visual aid, not a crutch. Even the most prepared of us can find ourselves talking to our...navigate questions Answering questions is arguably the most difficult part of a poster session, but with a little...which posters you are interested in viewing. For most larger conferences, poster titles and abstracts ...to the session, so bookmark which posters are of most interest to you a few days in advance. If you don...website. Time permitting, you can go back to the most interesting and popular posters at the end of the...

...delivery and gene therapy and represent one of the most studied viruses. Adenoviruses are non-enveloped ...isogenic AdVs encoding TALENs targeting the same chromosomal region. The CRISPR/Cas9-derived RNA-guided nuclease-induced... will interact with sporadic double-stranded chromosomal DNA breaks “that always happen naturally.” These...Muscular Dystrophy, Alpha1-antitrypsin deficiency (most widely used so far) or for cancer gene therapy such...Current state and clinical perspectives. Biochemistry Moscow 81:700–708 . https://doi.org/10.1134/s0006297916070063...Ventura A (2014) In vivo engineering of oncogenic chromosomal rearrangements with the CRISPR/Cas9 system. Nature...

...retroviruses have been developed into some of the most widely used tools in molecular biology. “Retroviruses...of their more complicated genomes. Here, we will mostly use the gamma-retroviruses as a simple spokesmodel...molecular biologists working with vectors are, in most cases, interested in transferring genes to mammalian...double stranded DNA is integrated into the host chromosome, and this is where viral vectors and natural ...express. In the case of a natural virus, this new chromosomal locus is considered “proviral DNA” (Rowe et al...Resources on addgene.org Addgene's Lentiviral Guide Most popular lentiviral plasmids γ-Retrovirus Guide ...

...change, the safety of genetically modified organisms (GMOs), the absence of a link between vaccines and autism...Association for the Advancement of Science) believe GMOs are safe for human consumption in comparison to ...active research scientists believe climate change is mostly due to human activity while much less of the general... of the word ‘fact’. Policy must be based on our most accurate interpretation of the world, and this interpretation...the following subjects: Climate change Vaccines GMOs Transgender issues Reproductive health Immigration...paired with long-term science outreach, may have the most profound impact (15). Diversity and inclusion ...

... expect: Search bar The biggest change is to our most frequently used feature: the search bar. We’ve changed... easy-to-use depositing guide, with links to our most helpful depositing articles. If you’re depositing...Figure 2: The new deposit materials bar New menu Most common activities can be done straight from the ...educational resources page, and previews for our three most recent blog posts. Finally, you’ll find everyone...discover as you browse. You may find one of your most frequently used buttons stands out better on the...

... is the best studied and most commonly used serotype. Its genome is the most common choice for AAV pseudotyping...adeno-associated viral vectors, or rAAVs, can sound almost too good to be true! In a previous post, we covered...the genome, and the second number to the capsid. Most vectors provided by Addgene are pseudotypes using...from one another. Common serotypes We’ll cover the most commonly used serotypes in this post. However, tissue...our in-stock viral prep service.  AAV5 AAV5 is the most genetically distinct AAV. Though AAV5 is one of ...

...questions can elicit the most valuable insights you will get from the interaction. Most people like to help...help us learn about the nonacademic sphere where most of us will be working.  Check out Joanne's Reddit... lists of questions on the internet. To make the most of the opportunity, use a written list and take ...stories. Thanks to the Benjamin Franklin Effect, most people actually enjoy helping (just ignore the people...

...candidate at the University of Cambridge. One of the most important steps in the CRISPR experimental process... a variety of options for CRISPR genotyping. The most common options include mismatch cleavage assays,...genome and sgRNA. This is a good starting point for most researchers as it provides a list of putative off-target...effects in the clinic. NGS-based methods provide the most complete information profile regarding putative ...comprehensive list of off-target editing events. Two of the most common cell-based methods are genome-wide, unbiased...using just genome-wide unbiased methods. However, most investigators use single cell clones for in vitro...

... job opportunities in academia - the main reason most people enter postdoctoral training. Similarly, there...example). This blog post is about how to get the most out of your postdoctoral training experience. If... biomedical postdoc fellowship lasts five years. Most universities and institutes annually advertise for...you how to write as well as mentor. Yet, even the most dedicated mentor is not always available for mentees... you or the audience. Ask yourself: What is the most exciting story I can tell with my data?  Can I make...this blog add up to a substantial amount of time- most of your waking hours. This is doable and it's a ...

...your experiments, I've described below some of the most frequently requested vectors in our repository and... consider as you make your choice. The first and most important thing you need to know is your expression...or a fusion protein. Mammalian vectors One of our most popular mammalian expression vectors, c-Flag pcDNA3... YGOI in an isolated, controlled environment. In most cases bacterial expression vectors are inducible... We have a variety of other types of vectors for most applications used to study gene function. From making...

...replicate the plasmid in addition to its own chromosomal DNA, costing additional resources to maintain... have also been used to disrupt genes at the chromosomal level. Scientists introduce an antibiotic resistance...Tips and tricks from the bench Use fresh stocks. Most antibiotics are stable in powder form, but quickly...and avoiding repeated freeze/thaw cycles will keep most antibiotics viable for at least 6 months. Ampicillin...Ampicillin and can be used in place of Ampicillin in most applications. Antibiotics vary in their sensitivitly...

...unusual ploidy and are considered hypotriploid: most chromosomes are represented by two to three copies, but...but several have four. No Y chromosome is present in this line, making it genetically female. HEKs are...suspension. Of the many derivatives of HEK-293, the most commonly used line expresses SV40 large T antigen...transcriptome studies of the cell line have determined it is most likely adrenal in origin. (Anatomy refresher: the...