Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9
Type
Blog Post
Published
Dec. 16, 2015, 3:30 p.m.
...of the Cas9 nuclease (PDB ID: 4OO8 and 4UN3), Slaymaker et al. hypothesized that Cas9 cutting efficiency...enough to keep off-target sites from being cut. Slaymaker et al reasoned that, if they decreased the positive...the electropositivity of the HNH/RuvC groove, Slaymaker et al. made a variety of alanine substitutions...off-target effects genome-wide.
Further work by Slaymaker et al. showed that these mutants are not toxic...SpCas9-HF1, and HypaCas9 should enable researchers to make precise edits in mammalian cells and may decrease...look forward to seeing how the research community makes use of these tools and are excited to see continued...improvements in genome engineering!
References
1. Slaymaker, Ian M., et al. "Rationally engineered Cas9 nucleases...