...Kanamycin aminoglycoside Binds 30S ribosomal subunit; causes mis-translation Bactericidal 50-100 ug/...Spectinomycin aminoglycoside Binds 30S ribosomal subunit; interrupts protein synthesis Bactericidal 7.5... Erythromycin macrolide Blocks 50S ribosomal subunit; inhibits aminoacyl translocation Bacteriostatic... Tetracycline tetracyclin Binds 30S ribosomal subunit; inhibits protein synthesis (elongation step) ... ug/mL Chloramphenicol   Binds 50S ribosomal subunit; inhibits peptidyl translocation Bacteriostatic...

...database design (schema).  Registered users can submit new fluorescent proteins, add data to existing ...database is free from the risk of inaccurate data submissions, so FPbase includes a versioning system that ...and to view previous versions.  Currently, all submissions also require a DOI referencing primary literature...published literature as soon as possible after submission. Using FPbase The most obvious use case for FPbase...experience in Python, fork the project on github and submit a pull request. Many thanks to our guest blogger...

... own subclone, they were able to develop a 12-plex multiplex imaging system. Mixing the subclones within...Every few months we highlight a subset of the new plasmids and viral preps in the repository through ... a fluorescent protein coupled to a specific subcellular localization peptide. By combining three different...their work with the “signalome,” a 12-plex mixed-subclone system using mStrawberry, localization peptides...

...Monitor neo-substrates Small molecule degraders may recruit CRBN to nonstandard or “neo-substrates.” We advise...evaluation of degradation of known cereblon neo-substrates (IKZF1, IKZF3, GSPT1, ZFP91, and CK1a). While...While expression of these cereblon neo-substrates varies in different contexts (for example, IZKF1 and IZKF3... to date, we have not observed off-target neo-substrate degradation with dTAG-13. Example experiments ...

...spaced CRISPR software submissions. With few prior publications to go on, a submitted manuscript could only...few moments after the piñata burst, value was subjectively ascribed relative to the basic needs of each...software design that is applicable to only one, or a subset of organisms. In only a few cases the software ... agnostic. Most tools are exclusive to one or a subset of organisms. Examples: “organism agnostic”, “model... you can rule out any tool that limits you to a subset of specific genomes. These are most of the tools...

...Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids...distribution of clusters without accumulation in subcellular compartments such as nuclear speckles. Furthermore..., by fusing a fragment of the kainate receptor subunit to the C-terminus of the protein. Unlike the diffuse...construct in which TagRFP-T and Dronpa flanked a PKA substrate fused to the phosphopeptide binding domain FHA.... The FHA1 domain binds to phosphorylated PKA substrate, inducing a conformational change that placesTagRFP-T...

...Did you know that one of the quickest ways to submit deposits with more than 10 plasmids is to use our...the page, you’ll see two options for data entry: “Submit Online Deposit” and “Download Deposit Spreadsheet...Figure 1: Begin the deposit process by clicking “Submit Online Deposit”or “Download Deposit Spreadsheet...Plasmid sequences or GenBank files. We encourage submission of QUEEN-generated GenBank files (Mori and Yachie...Download a new spreadsheet each time you want to submit new plasmids! We update the spreadsheet every so...

...they are looking for in grant submissions and will direct you on how to submit your work either through seminars...writing and hopefully increase your success rate. 1. Submit your grant early! First off, and perhaps the most...for you to make the relevant alterations before submitting - handing them your application 3 days before...their scoring system or meeting with their grant submission panel. Some funding bodies provide opportunities...

...The blood-brain-barrier (BBB) exists to prevent substances in the blood, like proteins and viruses, from...mechanism unknown)   Chen et al., 2023 AAV9.452sub.LUNG1 Lung: ATII cells Mice N/A Goertsen... encompasses more molecularly-defined neuronal subtypes and with less bias among them than AAV-PHP.eB ...type II cell transduction in the rodent lung AAV9.452sub.LUNG1 is an engineered capsid derived from AAV9 ... does express in the brain. Both AAV9 and AAV9.452sub.LUNG1 have significantly higher off-target expression... validation, validation! (psst… don't forget to submit your validation data to our Data Hub once you’re...2021). Enhancer viruses for combinatorial cell-subclass-specific labeling. Neuron,109(9), 1449–1464.e13...

...chemiluminescent substrates are available. Labs with limited equipment often opt for the chromogenic substrates since...For enzymatic signal detection, users provide a substrate that the enzyme converts into a detectable product...antibody. The reporter reacts with a provided substrate and produces a signal that is directly proportional...enzymatic reaction between the detection antibody and substrate is proportional to the amount of antigen present...

...After production, all virus preps are titered and subjected to quality control by Addgene before being distributed...VP2, and VP3. Samples of viral preparations are subjected to polyacrylamide gel electrophoresis (PAGE) followed...Silver staining of purified and non-purified AAV subjected to gel electrophoresis. Viral capsid proteins ...lentiviral preparations (described above) that are subject to a concentration step prior to being frozen. ...gene and the cellular ribonuclease P/MRP 30 kDa subunit (RPP30) as a control for normalization purposes...

...variety of topics. Individual forums are hosted on subreddits (r/”topic”) which are essentially lists of forums...Software for Biology) on Reddit and got some substantial traffic that we thought it would be a good idea...engaged, and has great questions! 2. The r/Science subreddit is well moderated. One of our original worries..., and even coached us on how to write our AMA submission to avoid rousing the trolls. 3. The AMA process...organized. You can check out the r/Science AMA Submission Guidelines here, but essentially we just needed...

...or more copies of the same plasmid (Bedbrook & Ausubel, 1976). This recombination event produces a multimeric...therefore run higher on an agarose gel (Bedbrook & Ausubel, 1976). In fact, if your plasmid sample has a mix...the formation of plasmid multimers (Bedbrook & Ausubel, 1976; Fishel et al., 1981). E. coli strains such...independently increase the frequency of base substitution in human cells. J Biol Chem. 2011 Mar 25;286....1021/bi00850a028. PMID: 4878700. Bedbrook JR, Ausubel FM. Recombination between bacterial plasmids leading...

...negative control is a sample or group that is not subjected to the experimental condition. In the example ...control could be a third group of mice that is subjected to the experimental protocol but does not receive...knockout.  If knockout samples are unavailable, try substituting with knockdown approaches using siRNA or shRNA...either by modifying the blocking conditions or by substituting for a more specific secondary antibody. The ...Immunoblotting and Immunohistochemistry: A Guide for Submission to the British Journal of Pharmacology.” British...

...identified subpopulation. For traditional cell culture, enriching your active RNAi subpopulation can be easily...necessarily dictates the direction and scope of the subsequent two. Consequently, I will focus on different ...gene silencing efficacy, thereby requiring a substantial time investment. Once the target mRNA is cleaved...and visual screening method was limited in two substantial ways: variable silencing efficiency and labor-intensive...

...same bottle of serum again and again for each subculture can enhance the growth of mycoplasmas. The serum...generated will enter the culture medium during subculturing processes or from the air if the cell line remains...source of the problem is the media, used for subculturing, which is available in both liquid and powdered...contamination present in the serum to be used for subculturing. Avoid using expired products or reagents that...viability of cryopreserved cells decreases with every subculture. Equipment Fumigate the laminar airflow and...

...itself. Many thanks to our guest blogger Subhadra Jayaraman! Subhadra Jayaraman is currently a doctoral candidate...This post was contributed by guest blogger Subhadra Jayaraman, a doctoral candidate at Binghamton University... ability to inhibit further cancer growth and subjecting patients to the risk of microbial infection. ...cytotoxic effector T cells, and targeting specific DC subsets in vivo using specific antigens to activate them...

... or does not result in a reward sensation and subsequent repeated stimulation. A spatial memory task, ...activation of which results in depolarization and subsequent firing of an action potential. Halorhodopsins... of which results in efflux of chloride ions, subsequent hyperpolarization, and inhibition of action potential...help restrict expression to a specific neuronal subtype. However, the length of promoter that can be used...identification of the bregma and lambda sutures and subsequent determination of the proper coordinates of your...

... lines when annotating a cell line Community submissions via email: [email protected] Entries include... lines is included when available, and can be submitted via email for cell lines profiled in the lab. ...available information about a cell line you’ve used? Submit your manuscript or data to Cellosaurus via cellosaurus...sib.swiss.This community resource is actively encouraging submissions from scientists all over globe, with some surprising...

... biological processes. Chemical effectors are suboptimal for a number of reasons, including their potential..., promoting transcription (see figure below). Subsequent exposure to red light rapidly deactivates the...computational problem of edge detection. Tabor et al. subsequently engineered a second photosensitive TCS activated...phosphorylation, promoting transcription of a GFP reporter. Subsequent exposure to red light rapidly deactivates the...