...service. Some AAV1 tools are available through our in-stock viral prep service.  AAV2 Despite being isolated...service. Some AAV2 tools are available through our in-stock viral prep service.  AAV5 AAV5 is the most genetically...service. Some AAV5 tools are available through our in-stock viral prep service.  AAV8 AAV8 has high transduction...as plasmids in the repository, as part of our in-stock viral prep service and can be selected as a serotype...service. Some AAV8 tools are available through our in-stock viral prep service.  AAV9 AAV9 has relatively broad...service, but some AAV9 tools are available as in-stock preparations. AAV retrograde (AAVrg, also known ...  Some AAVrg tools are available through our in-stock viral prep service.  In summary Your choice of ...

... maintain many fly stocks that are available to everyone. Flybase lists what stocks are available at Bloomington...start of the day and throughout daytime. They mostly stop emerging at night. An incubator with a light cycle...genetic crosses. More importantly, this knowledge will stop you from embarking on a fruitless journey of trying...information about gene function, while others offer fly stocks and other reagents. If you’re ever confused about...where the gene is expressed within the fly, whether stocks of mutant flies exist, whether any phenotypes have...mentioned the gene. The Bloomington Drosophila Stock Center at Indiana University is another tremendous...

...anniversary celebration, we’ve been finding and sharing stories unique to our repository and resources. As editor...picked our viral vector service as one of those stories. Viral vectors are viruses engineered to be safely...available and number of requests tell only part of the story. Viral vectors are, after all, a delivery method...blog, you may recall our first 20th anniversary story on CRISPR, where we dove fairly deeply into the ...primary literature, soliciting information from requestors and depositors, and identifying trends in our...actually show you if the vector was useful for the requestors or how it performed. Addgene launched our AAV...platform for the sharing of that exact data. Here, requestors can upload their experimental data in a standardized...

...norvegicus genes! Mexican axolotl (Ambystoma mexicanum) Ambystoma mexicanum, aka the axolotl. Image...are five more model organisms that have left a historical impact on the scientific community. (And, check...  The E. coli genome has an extensive research history and characterization. Many proteins and processes...organisms in the world. With its extensive scientific history, highly characterized genome, and widespread utilization...organisms is its thoroughly developed and defined history in research (Krämer, 2015). As a result, a vast... https://doi.org/10.1159/000490898 Gibbs, R., Weinstock, G., Metzker, M. et al. Genome sequence of the...

...copies of a tRNA that specifically recognizes amber stop codons (TGA) to encode phosphoserine, the Sep-tRNA... the phosphorserine OTS recognizes the TAG amber stop codon, using the OTS in an unmodified bacterial ...would result in read-through translation of many stop codons and detrimental effects on cell growth. The...was genomically recoded to eliminate all 321 amber stop codons (TAG), replacing them with TAA (7). This ...TAG codon should encode phosphoserine instead of a stop codon, which will allow full length GFP to be translated...can also be cloned with an ORF of interest for customized experiments. Once the ORF of interest is cloned...suppression, as described (6). To determine the stoichiometry of phospho-incorporated to non-phospho-incorporated...

...individuals through stock or investor pay outs. Addgene has no investors or stock. All of our proceeds...processes, distribution, and customer service) Addgene provides unmatched customer service that is consistently...

... chose the stop motion format for our videos as it is the most approachable for LEGOs. Stop motion consists...pictures together to form the full movement. Producing stop motion videos is very time consuming; producing ...toys” gaining life and working as scientists in a custom made, colorful laboratory. When we started, the...child to work. If you have an odd or quirky lab story you’d like to see portrayed in LEGO form, feel free...us at [email protected]. Who knows, your story might just get “legofied.” Many thanks to our guest...

...research project must have innovative yet attainable milestones that contribute to intellectual thought and discovery... The research project also serves as a stepping stone into the next professional stage of your career ... and skills do you need to use to reach these milestones? Can you get assistance from people who have ...for guidance, intelligent discussion, and/or brainstorming sessions to maximize productivity and direction...Think about what your personal goals are and set milestones along the way so that you don't get stuck doing... Passion fuels the drive to continue persuing milestones and working hard. Being patient, being selective...

...negative” data We all want to be able to tell a good story when presenting scientific findings. This makes ...of information and, from a SciComm perspective, stories with links of causation from initial hypothesis...validation are easier to understand. The need for great stories has, however, made it difficult to publish so-called...original hypotheses. Looking for novelty in our stories, we are additionally averse to producing papers...published results. This over-reliance on the perfect story for a “high impact” publication can lead to the ...the tested hypothesis is correct (a great success story) even though the majority of the unpublished evidence...generate can, however, bias us. We’ve all heard stories and read papers wherein a researcher tries 5 separate...

...Leiwe and Takeshi Imai from Kyushu University. Stochastic multicolor labeling is a powerful solution for...microscopy-based neuronal reconstruction. To achieve stochastic multicolor labeling, Brainbow used the Cre-loxP...copies of the transgene cassette are introduced, stochasticity will result in a combinatorial expression of...Brainbow was inherently low. This is because the stochastic and combinatorial expression of fluorescent proteins... such vector-based gene delivery systems, the stochastic expression of XFPs is possible by simply introducing...introducing a mixture of XFP genes. Stochastic multicolor labeling is possible when each of the XFP genes...

...exciting story I can tell with my data?  Can I make better slides that more effectively tell a story? Generally...publications (published, not in preparation) A history of successful funding (fellowship grants) A project...additional controls necessary? As you build your story, are additional data needed to rule out alternative...Before you do your first experiment in your new lab, stop and plan it out. Some questions to answer include...presentation. Yet, PFs need to tell and sell their stories to get jobs. PFs (and mentors) can never be too...question is yes. As you get better at telling your story, you can find clearer ways to consolidate or present...

...widely varied with many properties, having well-understood standardized terms makes it a lot easier for ...plasmids at Addgene are shared under, is well-understood and accepted by the majority of research institutes...Recipient (in Addgene parlance, the depositor and requestor) organizations. Over the last twenty years, we...your organization agrees to let Addgene receive, store, and distribute your material under the specified...move forward with shipping the material to the requestor.  Why the MTA?  The MTA process can be a bit confusing...exceptionally fast tech transfer offices with the Golden Stopwatch award and seek feedback as to how we can make ...

...offerings! We recently re-amplified our distribution stock of the Brunello Human gRNA library, and we thought... a file in FASTQ format, a standard format for storing NGS reads and their corresponding sequencing quality...in an automated manner. Addgene commonly uses a custom script derived from the count_spacers.py file from...representation of your library. When plotted as a histogram, the counts will give you a bell curve of the ...finds that this representation is most easily understood visually. As an example, the curves from our ...screening, and if you have any tips, input, or stories of interest about library use, let us know in the...

...help win a business plan competition and advance investors to the next stage of diligence." In business competitions...non-scientific audiences. "There are 100 ways to tell a story - convince your audience you are doing amazing things...The team is key, and the initial team is the cornerstone of it all. Without a good nucleus, the company...are 100X better than current technology and get customers to change behavior. When you are ready to commit...crowd funding, federal funding, foundations, and investors are examples of various sources of funding. Always... Biology & the Importance of Assembly Standards Story of a SynBio Startup: RevBio's Epiphany (or Lack ...

...privilege to find and share what stories we can, and to hear the stories others have to tell.  "Also thanks...we wanted to walk through some of our favorite milestones and share some thoughts from the community that...six years to reach our first major repository milestone — 10,000 plasmids deposited and available — and... which was a major barrier for depositors and requestors alike. We transformed this process by aligning...Addgene reached the 500,000 plasmids distributed milestone! We were thrilled to see the impact our repository...

... Outlines a detailed scope of specific aims, milestones, possible setbacks, and potential alternative...Carefully read over all parts until all aspects are understood. While reading the funding description, pay attention...will address these limitations and lead to new milestones in the field. Research strategy The Research...aims The Specific Aims section highlights each milestone that must be met in order to deem your proposal...proposals consist of 3 to 5 aims, with secondary milestones to meet along the way to achieving each aim. ...comments may make it clear that the reviewer misunderstood the point that was being made, take it as an...

...advocating-for-science-boston-2016/. Sign Up For the Advocating for Science Symposium Boston 2016 Changes since...Science Symposium and Workshop 2016 – Sept 16-17 MIT, Boston The Advocating for Science Symposium and Workshop...also started progress towards solutions. The 2015 Boston FoR meeting was designed to explore areas where...various levels, with panelists including: Kate Stoll, PhD - Senior Policy Advisor MIT Washington Office...Professor of Sociology. University of Massachusetts Boston Christine Oslowski, PhD - Biotechnology Communications...

.... The viral genome DNA is further protected by histone-like proteins forming the nucleocapsid. B) Three...recommended to test for the presence of RCA in the viral stock by doing a plaque formation assay on A549 cells ...recombination for amplification. The final purified plasmid stock should be analyzed by endonuclease restriction digest...you’ll produce the primary recombinant adenoviral stock (rAdV-S). HEK293 cells are transfected with the ...lysate is your primary low titer rAdV-S, and can be stored at -80C for later use or used immediately for amplification.... Purified high-titer rAdV is then dialyzed and stored at -80C. Purification and concentration kits that...

...cause bad reactions in mice. Customization AAV offers a level of customization that is unmatched by other...over the past decade. CRISPR screens are easily customizable and enable the targeting of hundreds to tens...lentivirus but offer an improved safety profile, customization, and enhanced expression in many mouse tissues...combination of serotypes and psuedotypes means you can customize your CRISPR screen to a high degree. This is particularly...interrogate immune cells to improve immunotherapy in glioblastoma, and generate programmable screening platforms...

...proximity labeling and TransitID Tracking cellular history with protein ticker tape Teaching an old antibody...on-target delivery of genetic cargo  By Alyssa NeuhausTo improve delivery and reduce off-target effects....1101/2023.02.07.527548.    Tracking cellular history with protein ticker tape By Brian O’Neill The Cohen...created a system for recording the transcriptional history of a cell. Lin et al. generated a fusion protein...ticker tape images could recover transcriptional histories, accurate to within 40 minutes. Figure ...their initial tests with thick slice (70 μm) immunohistochemistry (IHC) (Figure 5). PSD-95 is a scaffolding...