... distribution of research materials. Our small shipping and handling fees drive our work. As we have grown...confidentiality where it is appropriate. This grows our relationship with our community members. We also find that...that, as a nonprofit, we attract partnerships with organizations that share our community-oriented values...Michael Lo., Michael Le., Michel, Michelle, Morgan, Natashia, Nicole, Olivia, Pamela, Racheal, Ramsey, Randall...Learn More About Our Mission Meet the Addgene Leadership Team Get the Latest News from Addgene ...

...scientific publishing world and debate issues about how to improve the scientific publishing process. There...as financially critical. Those who work on the publishing side of science have professional meetings, societies...Kitchen’ for a glimpse into the world of scholarly publishing. And see for example the Eighth International...science communications world, you might consider an internship or a short program to build skills and connections...

...We are excited about our new partnership with Allele Biotechnology which allows researchers to deposit...Because the excitation and emission maxima were red-shifted (462 nm and 492 nm, respectively), they gave this...further sought to identify why this protein was blue-shifted compared to its parent protein CFP484. Collaborating... that specific amino acid changes favored blue-shifting more than others. During this process, they identified...Central PMCID: PMC1698604. Ai, Hui-wang, et al. "Hue-shifted monomeric variants of Clavularia cyan fluorescent...

...from Kyoto University, and Marcus N. Leiwe and Takeshi Imai from Kyushu University. Stochastic multicolor...from Kyoto University, and Marcus N. Leiwe and Takeshi Imai from Kyushu University. Richi Sakaguchi is...anatomical maturation of the olfactory system.      Takeshi Imai is a professor at Kyushu University. His lab...2732. PubMed PMID: 26972009. Ke, Meng-Tsen, and Takeshi Imai. "Optical clearing and index matching of tissue...PMC4246963. Sakaguchi, Richi, Marcus N. Leiwe, and Takeshi Imai. "Bright multicolor labeling of neuronal circuits...

... 181-206. PubMed PMID: 17224625. Mizushima, Noboru, Tamotsu Yoshimori, and Beth Levine. "Methods in mammalian...PubMed Central PMCID: PMC4517063. Yoshii, Saori R., and Noboru Mizushima. "Monitoring and measuring autophagy...20144757. PubMedCentral PMCID: PMC2852113. Noda, Takeshi, and Daniel J. Klionsky. "The quantitative Pho8Δ60...

...framework If you can’t find internships at science companies, try to find internships with translatable skills...career: Try to get software product management internships, preferably on teams that run within the agile...project management  Bolster your skills in strong relationship-building and influence. You’ll need a large ...large deal of influence and strong relationship-building in order to rally many stakeholders behind a common...

...no better place for a scientist to make new relationships than at scientific conferences. Conferences ...this is a great ice breaker. Of course, real relationships go further and grow over time, but being interested...fun, memorable meal is a great way to build relationships. Make a list of experimental problems or questions...work – casual socializing is how people build relationships that are memorable. It isn’t always appropriate...Thesis Whisperer "Not Networking - Building Relationships for Success" webinar with Joanne Kamens via ...

...participants in skills useful for future mentoring relationships.   If you would like to start a group mentoring...for the time commitment of a formal mentoring relationship or there aren’t enough senior mentors available... the discussion. This is a chance to practice leadership and communication skills. The leader also provides...ideas on what to do once you are in a mentoring relationship.  Celebrate your successes and support each... come. Are you involved in Peer Mentoring relationships? Comment below with ideas from your own mentoring...

...010822 Nishimasu, H., Ran, F. A., Hsu, P. D., Konermann, S., Shehata, S. I., Dohmae, N., Ishitani, R., ..., Isomura, K., Kajimoto, S., Komori, T., Nishimasu, H., Shima, T., Nureki, O., & Uemura, S. (2018). Real‐time...they are completely unique to the CRISPR system (Nishimasu et al., 2014). Table 1. Summary of Cas9 domains... the last base pair with an end-capping loop (Nishimasu et al., 2014). This cap is where the DNA:RNA heteroduplex...

... with each other: from formal mechanisms like publishing a research article, presenting a conference poster...access or feedback. One of the most significant shifts in scientific communication is the rising use of... public server independent from peer-reviewed publishing in a journal.  Accelerating Scientific Communication...while peer review is valuable, modern scientific publishing has a number of drawbacks, especially the increasingly...2019) A proposal for the future of scientific publishing in the life sciences. PLoS Biol. https://doi....

...excellent tactic for scientists making a career shift or who wish to improve their current job situation...scientists are aware of the benefits of coaching relationships and do not know where to look for a coach. Many...thinking about what your goals are for the coaching relationship. Do you want to change jobs? Grow your professional...the coach to define how you will know that the relationship has been successful – what are the desired outcomes...engagements and has many areas of specialty from leadership development to mentoring for women and under-...

...throughput work, it’s likely that you come into the lab wishing there was a machine that could make your life a...Beckman Coulter’s Biomek FXP. We had a previous relationship with Beckman Coulter and that certainly played...DNA as opposed to in live bacteria. For these shipments, we’ve needed to find a way to purify DNA from...vial fiIling The majority of Addgene plasmids are shipped as agar stabs in glass vials. Have you ever wondered...caps and push them into micronic tubes instead of pushing them in one by one. It seems very simplistic and...

...to give yourself plenty of time when writing a fellowship proposal. Yes, yes, I hear you saying “We know.... Become a science communicator More and more fellowship applications are asking for a 200-300 word explanation...explanation of your research for non-scientists. Most fellowships are funded by the general public (either through...assisting me with applications for much larger fellowships. Also, if you were awarded money to carry out... fellowship...

...university and alterations to the student-university relationship, along with, of course, union dues. Time will...common thread that has the potential to profoundly shift the academic science landscape: the treatment of...opinion on these changes, it is likely that the shifts caused to the practical and philosophical landscape...Science and the MIT Graduate Student Council, we shift our focus toward advocacy and action with sessions... Kate Stoll, PhD - Senior Policy Advisor MIT Washington Office Ben Corb - Director of Public Affairs ...

...distribution and deposits. (A) Cumulative CRISPR plasmids shipped by year. (B) Cumulative CRISPR plasmids deposited...by year. (D) Cumulative CRISPR pooled libraries shipped by year.   As of the end of 2023, Addgene had...even mix of plasmids (by age) are requested and shipped out each year.     Figure 3: Percentage...from labs in ~40 different countries, and we’ve shipped CRISPR plasmids to labs in ~100 countries.  The...distributed per year, or approximately 104 plasmids shipped per working day, including appropriate MTAs and...

...many of you have negotiated over the order of authorship for a manuscript? And that’s only in your scientific...negotiation skills will help you strengthen relationships with your colleagues and supervisors and lead...negotiation skills are key to maintaining and growing relationships with your colleagues within and outside of your... what you want and can also strengthen your relationships with others who know that working with you will...Conflict Resolution Time management Managing a team Leadership Resources on Addgene.org Check out our careers...

...mK-GO. Large Stokes Shift (LSS): Stokes shift (named after George G. Stokes) is the shift in wavelength from...FPs, Stokes shift is less than 50nm (often much less).  For LSS proteins, the Stokes shift is ≥ 100nm....

...chromophore concealed at the center of the structure, shielded from quenching by aqueous solvent. This tightly-packed...intensity and photostability of GFP. This also shifted its major excitation peak from 395 nm to 488 nm...S65T Increased fluorescence, photostability, and a shift of the major excitation peak to 488 nm F64L Increased...phenol component (cyan derivatives) Y66H Blue-shifts the wavelength (blue derivatives) Y145F Increases... aggregation at high concentrations T203Y Red-shifts the wavelength (yellow derivatives) A206K Interferes...

...careers, women in STEM, work-life negotiation, and mentorship, we decided to have her participate in an AMA...careers, women in STEM, work-life negotiation, and mentorship. We can direct people to the AMA in the future...whole point is to meet actual people to build relationships. I also like Twitter because I can get a little...important to find good mentors and incentivize good mentorship: AMA mechanics: Be prepared! The mechanics of... mentorship...

...HDR), a distinct cellular DNA repair pathway. Accomplishing this usually involves simultaneously introducing...characterize this phenomenon and found a general relationship between the distance of a mutation from the ...(and let them cut close).   Figure 2: Relationship between cut site to mutation distance and editing...mutations When thinking further about the distance relationship described above, we realized that, in addition...incorporation efficiency, we might exploit this relationship to direct the zygosity of the cells. This was...