...and a key step for that is sharing the data when an AAVs doesn't work. Sharing your negative data helps ...Addgene AAV Data Hub, the place for scientists to share their AAV validation data. The Data Hub contains...incredible AAV talks given by Connie Cepko and Ian Wickersham.     Submitting to the Data Hub If you've ever...others and, when published, will meet FAIR data-sharing guidelines. But what if my AAVs didn't work?  ...

..., developed and deposited at Addgene by Arlene Sharpe’s lab, allow for wide deployment of in vivo gene...Marty LaFleur is a Postdoctoral Fellow in Arlene Sharpe’s laboratory at Harvard Medical School and is interested...Montero Llopis, P., Haining, W. N., Doench, J. G., & Sharpe, A. H. (2024). X-CHIME enables combinatorial, inducible..., Al Abosy, R., Freeman, G.J., Haining, W.N., & Sharpe, A.H. (2019). PTPN2 regulates the generation of..., Coxe, D. J., Doench, J. G., Haining, W. N., & Sharpe, A. H. (2019). A CRISPR-Cas9 delivery system for...

...to quickly find what you’re looking for. Tip 3: Share protocols and spreadsheets with lab members Now ... software, there are many free and easy ways to share your documents with your colleagues. Start small...frequently. Copy these into an electronic file sharing tool, such as Google Drive or Dropbox, and file...file them into a folder that you plan to share. The programs will allow you to choose who should have access... stop back and let us know what worked for you. Share your best tips, tricks, and ideas below. ...

...from 47 different tissues. Researchers upload and share both their successes (recommended reagents) and ... with the recommendation. The goal is to easily share and find what works and what doesn’t while engaging...They particularly encourage people to upload and share their negative data. After all, they tell me, when...anyone who has used IBEX and wants to preserve and share their validation work. Everyone who contributes ...the Zenodo release in keeping with their value of shared ownership. Go check it out! References and Resources...

...community, sharing, and fun. The Addgene repository is built on the foundation of a community that shares reagents...and repeat - we’re proud and excited to finally share with you what we’ve been working on behind the scenes...much easier to get around our site.  Community, sharing, and fun Along with improving search and navigation..., fun, and promote our values of community and sharing.  One big feature upgrade? Blugene now has a full...

...and share user data on the antibody webpages. These outlets can be particularly useful for sharing negative...protein, allowing you to proceed with confidence.  Share your results Ultimately, like all of science, validation...validation experiments don’t mean anything unless you share the results with others. If you publish data generated...antibody’s trustworthiness. You may also consider sharing your validation attempts by other means, such as...antibody (perhaps one from Addgene!), consider sharing your results so that others in the community can...

...CRISPR systems can be as confusing as reading Shakespeare for the first time. The discovery and application...as it seems. There is not a single gene that is shared across all CRISPR types; the fast evolution of ...systems that are in the same class and type don’t share sequence similarity but are grouped due to mechanisms.... S., Wolf, Y. I., Alkhnbashi, O. S., Costa, F., Shah, S. A., Saunders, S. J., Barrangou, R., Brouns, ...Horvath, P., Moineau, S., Mojica, F. J. M., Scott, D., Shah, S. A., Siksnys, V., Terns, M. P., Venclovas, Č...., Garrett, R. A., Randau, L., Sørensen, S. J., & Shah, S. A. (2019). Type IV CRISPR–Cas systems are highly...

...Hodges Sharon Dent Archa Fox Elaine Fuchs Kai Ge Robert Kingston Thoru Pederson Phil Sharp Julie Ahringer...! And if you study chromatin and want to start sharing your plasmids through Addgene, don’t hesitate to...spanning 20 years of research. DNA Cross-Bridging Shapes a Single Nucleus from a Set of Mitotic Chromosomes.... 2015 Jan 15;29(2):197-211. DNA Cross-Bridging Shapes a Single Nucleus from a Set of Mitotic Chromosomes...

...about five years ago. I was interested in what she shared about the company and what the goals were and so... to the scientific community through Addgene’s sharing platform. Expanding further beyond the US and increasing...lawyers. While there will always be an interest in sharing, there will always be an interest in protecting...challenge.Paratore: The promise there is the ability share the information, making it available to everyone...efficiency through which knowledge is created and shared. Can you tell me a bit about your job? Katz: I’...

... we were closing in on the one million plasmids shared milestone in 2018, we wrote a post on the time ...plasmid. In 2023 (and at closer to 2 million plasmids shared than not!), we think it’s about time we updated... repositories Repositories such as Addgene help share materials quickly and cheaply. We save you from ... free for you, and many labs are quite happy to share, labs that get high volumes of plasmid requests ...cloning method Find blog posts about scientific sharing here Download the Plasmids 101 eBook Resources...

...lab has developed two new FLIM-FRET acceptors, ShadowG, a novel dark green fluorescent protein (GFP) with... with an absorption peak of 486 nm and ShadowY, a novel dark yellow fluorescent protein (YFP) with an ...fluorescence lifetime imaging microscopy) assays. ShadowG is darker than other dark YFP mutants, it reduces...desired due to its very low quantum efficiency. ShadowY has a lower quantum yield and higher absorption...reduced artifacts due to residual fluorescence. ShadowY was additionally shown to have a 7-fold lower quantum...

...unpublished data they may ask the audience to not share their research on social media. Use the hashtag ...live-tweeting any sessions that the presenter doesn’t mind sharing on social media. If you could not attend some of...as well. In addition to using social media for sharing new scientific information from the conference,...business,” but having business cards is a good way to share your information quickly. During the conference ...the Addgene blog Read other posts on scientific sharing, scientific communication, and scientific networking...

...perform functional tests. The newest genome engineer sharing the stage with much-lauded CRISPR-Cas9 is DNA-guided...difficulty with the NgAgo system. I encourage you to share your results and protocols with the research community...specifically set up a forum where NgAgo users can share protocols and tips Twitter - Tweet suggestions and...who have been lucky with NgAgo should explicitly share tips and protocol details with the scientific community...biotechnology (2016). PubMed PMID: 27136078. 2. Burgess, Shawn., et al. "Questions about NgAgo." Proteins & Cell...

...unrelated to the issue and consider how it could be shaped to solve your problem. In a study outlined in Originals... assure that all participants feel comfortable sharing their ideas? And how do we give people the opportunity...s advocate is elected, others may feel they can share opposing opinions as well. I am sure we have all...time. Meetings shouldn’t be the only place people share ideas. Ideas had over lunch are just as valuable... The purpose of this form was to encourage the sharing of ideas, not to give people more work. Identify...

...have been shared over 140,000 times to over 75 countries. That’s a lot of CRISPR! As we’ve shared these tools.... This week, we’ll continue to grow the ways we share science by hosting our first ever CRISPR Week on...think so we’ll ask you some questions on Friday - share with us how you use CRISPR. Hope you will join us...

...neuronal connectivity in the mammalian brain (Wickersham et al., 2007; plasmids at Addgene). AAV vectors...al., 2017), and these two modes of transport may share common mechanisms. Rare observations like these ...2014). Adeno-associated virus serotypes 1, 8 and 9 share conserved mechanisms for anterograde and retrograde...Frank, D., Kajikawa, K., Mise, N., Obata, Y., Wickersham, I.R., and Tonegawa S. (2014). Cell type-specific...Tanaka, M., Kearney, M., Chattree, G., Yang, C. F., Shah, N.M., and Mooney, R. (2017). Identification of ...Neurosci Res 93, 144-157. PubMed PMID: 25240284. Wickersham, I.R., Lyon, D.C., Barnard, R.J.O., Mori, T.,...

...The best part is, we’re not only sharing them with you, but you can share them with your coworkers, labmates...labmates, and friends as well!   Addgene is sharing so much more than plasmids these days. via GIPHY...out everything we’ve uploaded so far, and start sharing the science love today!        ...

... always, a big thank you to our depositors for sharing your science with us! Corralling and Cornering ...antibodies for use in mice and human brain IHC and sharing the data on the mAb3D atlas website.  The iGluSnFR3...Abudayyeh and Jonathan Gootenberg worked with us to share their new CRISPR tools, Cas7-11, a low-toxicity ...work!) If you've got a research story you want to share on our blog, please reach out to [email protected]...

... post on Instagram                     A post shared by Xi Chen (@seleena78) The lab has officially... post on Instagram                     A post shared by 草方 (@gracie_fang) That’s right, researchers...need only decorate your lab, take photos, and then share them on Twitter or Instagram with #DeckTheLab and... post on Instagram                     A post shared by Xi Chen (@seleena78)   Here’s how to enter:...

... during funding transitions. Enabling resource sharing: Our operating model allows researchers to access...strengthening our commitment to collaboration and resource sharing, we can navigate this difficult period together... Want to Hear from You We invite researchers to share how these funding changes are impacting your work...Addgene during this time. Your input will directly shape our response initiatives as we stand with the scientific...