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We narrowed to 402 results for: LARGE

Showing: 61 - 80 of 402 results

  1. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    Published
    March 27, 2018, 1:32 p.m.
    ...to efficiently deliver large repair templates for applications that require large gene fusions. The current...Matthew H. Porteus. "CRISPR-mediated integration of large gene cassettes using AAV donor vectors." Cell reports...

  2. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ...guides directed towards that gene as there can be a large difference in guide potency. Thus, in cases where...pathways and genetic interactions. Additionally, large scale CRISPR-based screens can be used to gain insight...target effects are generally not regarded as being a large problem for Cas9 activators. This is believed to...makes it much cheaper to use Cas9 activators. This larger scale of activation allows for exploratory-based...

  3. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    Published
    Aug. 2, 2016, 2:30 p.m.
    ...more efficiently amplified than larger constructs, but plasmids as large as ~6 kb can be amplified fairly... This is particularly relevant in cases where a large plasmid with multiple functional regions is used... if you want to introduce a point mutation in a large construct. Particularly, if a pair of unique restriction...visible in a digest of the unmodified plasmid into a larger band (not shown). As a footnote; a similar approach...site, can identify these duplications (slightly larger bands sizes relative to the original template)....

  4. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    Published
    July 10, 2018, 1:08 p.m.
    ...solution that fits the most labs, our team conducted a large study which focused on the adoption of ELNs in labs...talking about ELN costs and limited budgets, “a large percentage of survey respondents indicated that ...support that. In addition, if you are working for a large institution or company, site licenses might be available...

  5. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    Published
    July 30, 2024, 1:15 p.m.
    ...and to verify the quality of the prep. This was a large factor in Addgene’s decision to launch our viral...category, available from Addgene as of 2023. A large percentage of viral vector preps contain biosensors...were distributed in 2023 alone. You’ll also find a large number of requests for vectors in the “controls”...

  7. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type
    Blog Post
    Published
    Sept. 14, 2021, 1:15 p.m.
    ...recommendations from the depositing lab. If your vector is large (>10 kb) or has a low copy number, try following...agar plates, you may notice that there are both large and small colonies. Bacteria containing the recombined...

  9. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    Published
    Aug. 25, 2015, 2:30 p.m.
    ...story altogether? The unfortunate answer is yes, largely due to a few limiting factors.  To start, the average...different than what was originally planned, due in large part to the feedback we received from our network...

  10. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    Published
    May 6, 2014, 1:07 p.m.
    ...optimized by testing various linker configurations in a large-scale functional screen in bacteria. Sensor variants...quite a robust way to make a FRET sensor with a large dynamic range,” he says. Merkx recently applied ...

  11. CRISPR Guide Update

    Type
    Blog Post
    Published
    Oct. 10, 2024, 1:15 p.m.
    ...and additions describing CRISPR’s ability to make large genome edits using transposases or the Cascade-Cas3...

  12. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    Published
    Sept. 8, 2015, 2:30 p.m.
    ...enable the assembly of larger systems. The work of Schmidl et al. also represents a large step forward in optimizing...required per system. Applications of these systems The large dynamic range of these systems ensures that they...

  13. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    Published
    Aug. 7, 2014, 1:58 p.m.
    ...thing to consider is the size of the conference. Large conferences have many opportunities but can be overwhelming... overwhelming as far as meeting new people. At large conferences, look for smaller events or meet-ups ...national recognition and the more people you know, the larger your profile in the community Job hunting outside...

  14. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    Published
    Dec. 10, 2013, 3:13 p.m.
    ...Musunuru: We have been using the kit to target a large number of genes involved in cardiovascular and metabolic...power here is that you could potentially sequence a large number of individuals and find several mutations...

  15. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ...time intensive to process a large number of microbiome samples. To enable large-scale, longitudinal studies...

  16. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    Published
    May 21, 2019, 12:10 p.m.
    ... scientists need a protease reporter that has a large dynamic range and high brightness. The Shu Lab from...FlipGFP is a novel fluorogenic reporter that has a large dynamic range and high brightness. This reporter...

  17. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    Published
    Sept. 24, 2019, 12:55 p.m.
    ...sequence, or if you want to amplify sequences from a large genome organisms like mouse or human. There are ...difficulties in amplifying a template, particularly from a large genome, you can recheck your primer design or optimize...

  19. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    Published
    May 26, 2020, 1:15 p.m.
    ...site in the genome you are trying to target play a large role in Cas9 activity and efficiency (Sander and... structure and genomic recognition sites play a large role in CRISPR-Cas9 efficiency. They continued to...

  20. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    Published
    June 2, 2015, 6:06 p.m.
    ..., gRNA and pol III terminator. These relatively large cassettes (considering the gRNA itself is ~100 bases...frequency of both indels at each locus, as well as large deletions. They detected fragment deletion with ...
Showing: 61 - 80 of 402 results