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A look at the history of the CRISPR/Cas system. Discovered in bacteria, this system has been harnessed for genome engineering.

Nov. 25, 2013, 10:12 p.m.

Where did plasmids come from? Learn more about natural plasmids, the benefits they offer cells, and how scientists have adapted them into laboratory tools.

Oct. 8, 2024, 1:15 p.m.

Use anti-CRISPR proteins to turn off genome editing and prevent off-target effects. In this article, read about how it works and its applications

July 23, 2020, 1:20 p.m.

Learn about antibody engineering, including what it is and why we like it. Deep dive into antibody directed evolution techniques, and learn about Addgene's partnership with the Institute for Protein Innovation (IPI).

Aug. 5, 2025, 1:15 p.m.

High schoolers invented a new diagnostic for Lyme disease. In the process, they also created a tool for jointly designing RPA primers and Cas12a sgRNAs.

May 12, 2026, 1:15 p.m.

Learn about RNA interference and how you can use shRNA plasmids to knockdown gene expression!

June 17, 2025, 1:15 p.m.

This guide on single chain fragment variables (ScFvs) covers how ScFvs are made, the advantages and disadvantages of using ScFvs, and several applications.

June 3, 2021, 1:15 p.m.

Learn how CRISPR-based diagnostic systems can easily detect nucleic acids of choice with high sensitivity, specificity and portability.

April 16, 2020, 1 p.m.

Learn how you can use kinase translocation reporters to monitor kinase activity at the single cell level.

July 26, 2018, 12:46 p.m.

Choosing a CRISPR activator? This article features four of the most popular CRISPR activators (dCas9-VP64, SAM, SunTag, and VPR) and some newer activators like dCas9-CBP and SPH.

Oct. 6, 2020, 1:15 p.m.

Learn all about CAPTURE - a new CRISPR-based technique to study regulatory elements in their native chromatin contexts.

July 2, 2020, 12:40 p.m.

Multicistronic or bicistronic vectors contain IRES and/or 2A peptides to allow for coexpression of multiple genes from a single transcript.

Sept. 9, 2014, 8:20 p.m.

How do we know if a therapeutic antibody is a promising clinical prospect? In vivo and in silico methods can help us decide!

Feb. 13, 2024, 2:15 p.m.

Repositories provide fast access to wet lab research materials during COVID-19 such as cell lines, plasmids, and mouse strains.

Aug. 4, 2020, 1:15 p.m.

Ginkgo Bioworks and Addgene are synthesizing and distributing thousands of plasmids for COVID-19 research. Read to learn more about these constructs

May 19, 2020, 1:15 p.m.

Check out how you can use the top lentivirus and AAV from 2016 in your own research.

Jan. 6, 2017, 3:56 p.m.

Check out some of the new and useful tools recently deposited in the Addgene repository!

Aug. 27, 2024, 1:15 p.m.

Vectors containing the piggyBac transposon are now available at Addgene. Learn how these unique tools can be used for genome engineering!

May 31, 2016, 3:30 p.m.

Dr. Le Cong, first author on a seminal CRISPR article from Dr. Feng Zhang's lab, answers FAQs about the genome editing technology.

March 13, 2014, 4:08 p.m.

Looking for a plasmid that can thrive in a number of different bacteria? Learn about broad host range plasmids in this post!

April 11, 2024, 1:15 p.m.