... for their essential advice and service. Joanne shared a moving thank you email from a requestor in Iraq...was once a complicated process of requesting and sharing plasmids has become simplified through Addgene.... our impressive stats from 10 Years of Plasmid Sharing Or read more about what happens Inside Addgene ...

...greatest good you can do for another is not just to share your riches but to reveal to him his own." -Benjamin...fulfilling, productive relationships. I will be sharing what I’ve learned along the way in this ongoing...relationships with advisors and others who helped shape their experience. This led to the topic of “What...

...possible before,” said the Broad Institute's Ophir Shalem. “Whole regions of the genome which were not accessible...depends on your experiment and method of selection. Shalem and Sanjana say they kept a high representation... CRISPR-Cas9 Knockout Screening in Human Cells. Shalem O, Sanjana NE, Hartenian E, Shi X, Scott DA, Mikkelson...

...communication In between recurring meetings, you should share interesting results or ask questions to your collaborators... using rapid communication tools such as Slack. Share data and documents While having multiple scientists...may sound like something out of your nightmares, shared ownership of materials produced from your collaboration...

...Rachel Leeson. Here at Addgene, we like to help share useful new resources with the scientific community... can be adjusted based on query, reference, and shared allele search preferences to help you identify ... some surprising, and important, findings being shared. For instance, recently Cellosaurus received a ...

...Addgene fulfill its mission of helping scientists share information and, of course, plasmids. My primary...communication but thought that I would be best poised to share the stories and difficulties behind great discoveries... be therapeutic and I’ve certainly done my fair share of venting, I’m glad I did a PhD and even more glad...

...modeling and structure-guided direct evolution, Nathan Shaner and colleagues from The Scintillon Institute, Allele...mNeonGreen, mTFP1, or mWasabi that you would like to share with the scientific community, please deposit the...PMID: 18325109. PubMed Central PMCID: PMC2292683. Shaner, Nathan C., et al. "A bright monomeric green fluorescent...

...critique and figure out what to improve. You can share it with others and get their input as well. Giving...Candidates who have “started something” which they can share on their resumes have a definite advantage in our...with someone who has interesting experiences to share. The conversation builds a relationship for the ...

...built off of the concept of our AAV Data Hub) to share further validation data with you. “Affinity reagents...allow Addgene to further our mission of scientific sharing,” says Dr. Daniela Bourges, Addgene’s interim Executive...transparent resource where researchers can easily share, edit, and access the affinity reagents they need...

...six of us. We were initially strangers but we all shared a passion for DIY Biology and, on occasion, would...plant cell culture and transgenesis he wants to share his knowledge with the community by teaching a DIY...Interview with Darrell Kotton Learn How Plasmid Sharing Speeds Science Resources at Addgene.org Order...

...roles in fluorescent protein development by openly sharing their resources with the research community. To...scientists attend a conference of their choice to share their fluorescent protein based research. This year...thaliana - Pantoea agglomerans interaction. But the shape and metabolism of leaves can be changed by light...

...for further investigation of this pathogen. The sharing and distribution of cell lines from repositories...scientists are able to make when they have access to shared materials, and then pay it forward by making the...databases and resources Learn about Addgene's work to share plasmids to all scientists Resources on Addgene.org...

...class twice a week at Addgene HQ, but now we’re sharing links to online yoga classes. Get outdoors. Taking...activities like team lunches, a virtual soup lunch, and sharing photos of our pets. #SocialDistancing may send... and CV. Attend virtual workshops and webinars. Share your work online. Maybe you’ve always wanted to ...

...else to follow them, to being open and willing to share with other researchers to help them troubleshoot... and understand. All of this communication and sharing fits together to make for a transparent and collaborative... for data management plans and for evidence of sharing for grant renewal—but there is clearly room to ...

... visiting scientist is distinct from equipment sharing or a collaboration – it requires re-location of...the conversation. Many researchers are open to sharing knowledge and establishing collaborations, so it...priority list of experiments you hope to complete. Share this plan with your host prior to arrival so that...

... a radioactive tracer. As a solution, Mikhail Shapiro’s lab has developed four acoustic reporter gene ...water from entering their hollow interiors. The Shapiro lab discovered in 2014 that these gas vesicle proteins...immune cells has been a gap in the field. Arlene Sharpe’s group at the Harvard Medical School developed ...

... followed soon by GCE done in mammalian cells (Shandell et al., 2021).   How it works If you’re interested...their phosphoserine ncAA protocol, to optimize and share, they’re soliciting feedback from scientists on ...182–188. https://doi.org/10.1126/science.2649980 Shandell, M. A., Tan, Z., & Cornish, V. W. (2021). Genetic...

... navigate when mapping out a new project. We’ve shared some of the concerns that Revolution Bio had to...scientist, and how you were able to overcome them. Share your stories in the comments below! And if you have...developed a genetic tool for plant engineering, please share it with Addgene to both build the plant synthetic...

...powerful in almost any career. It allows you to share and build support for your ideas, invite others ...done a lot of public speaking, and I'm happy to share what I've learned along the way. Own your time ...performance art. You’re (usually) presenting either to share knowledge or to invite critique onto an ongoing ...

...excellent resource for research design is Kalpit Shah et al., “Do you know the sex of your cell?” I can...time—and sex can be hard to determine. To remedy this, Shah et al. provide a table indicating the sex of the...Addgene Blog Learn How Scientists Are Trying to Shape the Future of Research Learn How Notions of Scientific...

...will discuss where better data is needed to help shape and inform the overall structure of the biomedical...area include publishing the book ‘How Economics Shapes Science’ and contributing to the NIH’s Biomedical...PMCID: PMC4000813. 2. McDowell, Gary S., et al. "Shaping the Future of Research: a perspective from junior...

... barcodes within the resistant populations were shared by at least one other replicate; prior to treatment... “I spend a lot of time packing, shipping, and sharing protocols one by one,” and she is excited to deposit...Addgene because it is a “really great way of broadly sharing these kinds of reagents.” Here at Addgene, we’re...

...are different types of cellulose fibers, pattern, shape. All of that is controlled by regulatory networks...same guide RNA twice. The second problem was the shape of the response function or how the output changes...function of input. Usually that function is in the shape of an S; there is a narrow threshold like a switch...

...retrograde transmission (Etessami et al., 2000, Wickersham et al., 2007). Thus, RABV transsynaptic spread...due to lack of G protein on the viral envelope (Wickersham et al., 2007).   Summary and RABV applications...Jun 7;74(5):858-73. Pubmed PMID 22681690.  8, Wickersham IR, Finke S, Conzelmann KK, Callaway EM. Retrograde...

... Since Addgene is very proud to help scientists share plasmids across the globe, we’re working on ways...great lab automation tips and tricks, be sure to share them in the comments section.   Additional Resources... Shannon Rinaldi...

...Many thanks to our guest blogger, Harshana S De Silva Feelixge. Harshana S De Silva Feelixge is a researcher...This post was contributed by guest blogger Harshana S De Silva Feelixge. Gene therapy technologies ...

...Wormbase. And scientists have been continuously sharing their worm strains through the Caenorhabditis Genetics...C. elegans researchers embrace open science and share resources and tools. That message was fully exemplified...some examples of worm resources and tools openly shared by the worm community. For additional resources...

...difficult-to-engineer Sulfolobus islandicus rod-shaped virus 2 (Mayo-Muñoz et al., 2018). Only viral particles...CRISPR-Based Genome Editing of Sulfolobus islandicus Rod-Shaped Virus 2. Viruses 10:695 . https://doi.org/10.3390...Zhang Y, Garcia B, Hidalgo-Reyes Y, Lee J, Edraki A, Shah M, Sontheimer EJ, Maxwell KL, Davidson AR (2016)...

...experiments involve needles or scalpels (sharps). Cuts and scrapes from sharp equipment are one of the most common... yourself via needlestick or cut. Following all sharps procedures for your organization will significantly...

...metabolic pathways. Fertility (F) plasmids: microbial sharing F plasmids contain the genes for bacteria to form...new cloning techniques based on these vectors (Shareck et al., 2004). Plasmid categorization is complex... https://doi.org/10.1016/j.plasmid.2023.102684 Shareck, J., Choi, Y., Lee, B., & Miguez, C. B. (2004)....

...engaged in, these issues which will continue to shape the sector throughout their careers (McDowell et...available avenue. Interestingly, both of these changes share a common thread that has the potential to profoundly...PMCID: PMC4866822. 3. McDowell, Gary S., et al. "Shaping the Future of Research: a perspective from junior...

...speak about Addgene’s experience helping scientists share plasmids, especially those of the CRISPR variety...outskirts of Guangzhouon west of beautiful Mao Fengshan Forest Park. As a national science and technology...distinction of “higher” and “lower” life forms as we shared millions of years of common ancestry with worms...

...? Please share them in the comments below! Special thanks for Dr. Dan Stone and Harshana de Silva ...definitely made my life easier!  In this post, I’ll share some AAV production, purification, and titration...

... social media) and freely sharing code, raw data, and more. The team shares results early and often, including...impact,” he says, noting that his team regularly shares troubleshooting tips and works with other labs ...

...concentration of fluorophores) cast shadows along the illumination path. The shadows appear as stripes in the acquired...Klymkowsky. (1989) Development, 105.61-74. Pubmed. J. Sharpe, U. Ahlgren, P. Perry, Hill B, Ross A, J. Hecksher-Sorensen...

...for parents and other members of the community to share their expertise with the kids through some fun activities...Middle school: supplementing the science curriculum Sharing my expertise with our district middle school was... of the talk by running it by the teachers and sharing some slides on Twitter, where I was able to get...

...teleconferencing software. Any file sharing service should also serve to share the materials quickly and easily...easily ahead of and after the journal club. Sharing the materials serve two purposes: to allow those who...

...Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang F (2015) In vivo genome...KLM, Chuck J, Tan D, Knott GJ, Harrington LB, Al-Shayeb B, Wagner A, Brötzmann J, Staahl BT, Taylor KL,...

...when bacteria of different evolutionary lineages share genes that can improve chances of survival for an...experiences of the mentors were very valuable when shared with the next generation of scientists. The connection...Tiffany Lowe-Power, Eric Griffis, Janet Iwasa, Shawnna Buttery, Kat Milligan-Myhre, and Pragati Chengappa...

...That is, until earlier members of our lab - the Shapiro Lab - developed Acoustic Reporter Genes (ARGs),...studying bioengineering. They are both members of the Shapiro lab at Caltech.   References and Resources References...., Ho, G. H., Sawyer, D. P., Bourdeau, R. W. & Shapiro, M. G. Ultrasound imaging of gene expression in...