...transformed without a heat-shock step. Because of the large number of transformations being performed at Addgene...

... possible to quickly and efficiently assemble a large number of building blocks with just two or three...

...counter-selection. Anecdotally, there is also a large amount of user-to-user variability, and in some ...modification of bacterial genomes. It can accomplish large deletions, insertions, and even precise point mutations...

...companies now sell fast digest enzymes that can digest large amounts of DNA in as little as 10 minutes, but check...Additionally, if your final product is going to be very large (>10kb) you might want to use electro-competent ..., the DNA migrates toward the anode (+ charge). Larger fragments of linearized DNA migrate slower than...purification you can do is a miniprep, but if you need larger quantities of DNA, you’ll need to do a midiprep...

...genes for deoxyribose synthesis Allows uptake of large plasmids hee   High electroporation efficiency...Chloramphenicol High competency cloning and propagation of large plasmids, ligated DNA, and libraries, nalidixic ...

... responsible for cancer from genomics data, and large-scale patient cohort data from the MD Anderson Cancer...

...1) other strategies may not be feasible and (2) large-scale proteomic and transcriptomic datasets already...CRISPR/Cas9. You check the mutation and it causes a large deletion that removes the entire exon that codes...

...migration resistance and improve separation. For large proteins, reduce the percentage of acrylamide. To... and typically takes less than an hour. However large proteins typically do not transfer as well using...

...mutations behind. Furthermore, piggyBac™ offers a large cargo-carrying capacity (over 200 kb has been demonstrated...recombinase. However, the use of negative selection largely overcomes this obstacle. Furthermore, we have found...a cell line with the edit of interest. With the larger piggyBac™ donor vector, it is possible to make ...

... no good treatment. The dystrophin gene is very large (79 exons), but much of the sequence appears to ...lifespan. This work should be done in mouse and large animal models with mutations in human-relevant exons...

...correct phages will test. These includes labs with a large number of different phages or with phages targeting...

...system allowed us to handle and distribute such a large deposit. Once the project is complete, Ginkgo will...

...physical (blue) and social (orange)  scientists largely cluster by discipline, suggesting that scientists...the more friends they have in common. Only the largest connected part of the follower network is shown...

...single-molecule tracking Caveats of SunTag Very large size: a 24x scaffold fully occupied with GFP has...

... or p19 promoters to, respectively, produce the large (Rep78 and Rep68) and small (Rep52 and Rep40) Rep...

...collaborations and conferences for their students. Another large goal of the organisation is to encourage iGEM alumni...

...follow their passion. “There are many ways to have a large positive impact in society. We need much, much more...

... nm will pass through animal and human tissues largely unimpeded. The challenge then was to develop fluorescent... Ultrasound wavelengths, on the other hand, are larger than the size of a cell, so they scatter much less...

.... John Pringle and Jürg Bähler have deposited a large collection of plasmids for genome modification in...

...discovered. These proteins are characterized by large DNA recognition sites, which makes them highly specific...agriculture, its portrayal in popular fiction has been largely negative. But these negative depictions serve as...

...interest.   Advantages Disadvantages Can carry large transgenes (>8 kb) Potential for generation of replication-competent...have been developed, but these vectors are quite large and you might get lower viral titer when using them...

...indicates that the amount of leaky expression can largely be attributed to the level of spontaneous recombination...tested in the researcher's system before beginning large scale experiments. This will help to ensure the ...

... offer the most flexibility for expression in a large number of cell types and are useful if you're going...several different systems or want to transduce a large number of different cells. Promoters such as chicken-beta-actin...

...techniques like CLIP require a specific antibody and a large number of cells to analyze. A new technique from...trying to activate target genes, the relatively large size of dCas9-VPR limits one’s ability to package...their ability to generate both small (< 100 bp) and large (up to 120 kb) deletions in N. benthamiana and Arabidopsis...higher frequency and could be detected by PCR while large deletions were relatively rare. The vectors are ...human kinase domain constructs developed through a large-scale expression screen that can be used to generate...anchored to amylose resin for solid-phase synthesis of larger fusions through SpyTag/SpyCatcher and SnoopTag/...of target cells. The piggybac system has a much larger cargo capacity than lentivirus and is capable of...

...interested in exploring biological dark matter – large tracts of the microbial tree of life that remained...

...production can be a frustrating process that requires a large investment of time and resources for labs that don...

...more likely to closely read an email sent out to a large listserv or one sent to you and a few other people...

... for GUS staining or are not suited to studying large samples. RUBY Red But recently, something new has...

... regularly does immunoimaging, there’s likely a large collection of antibodies in your lab. Perhaps you...

...al., 2013). One issue with this strategy is that large complexes may not permeate through your tissue samples...

...protein) with amylose resin. The MBP tag, although large, can actually increase expression and solubility...

...that kill the target cell. If the pathogen is a large parasite, ADCC can be induced through a very similar...

...employee. This is especially true if the problems are large enough that the employee should be aware that they...

...specific mutations. For these purposes Addgene has a large collection of yeast expression vectors with various...

...self-interested reasons, and, given that science is largely funded by tax-payers, there are reasons to feel.... 4)   If you enjoy writing and are ready for a larger commitment, start your own blog. Plan to write ...

...how a protein of interest actually behaves. How large is it? What parts of it move when you feed it substrate...labeling reagents and structurally characterizing large ion channels involved in skeletal muscle contraction...

[email protected]. These deposits are considered large deposits and are exceptions to our standard workflow...your plasmid is designed to allow you to produce large amounts of your insert in E. coli, then this is ...

...FPs are tremendously complicated entities with a large range of characteristics (color, brightness, photostability...your help!), I believe this represents one of the largest available online collections of FP spectra. Every...

...to a lysine residue within the AviTag. However, large amounts of the target protein are typically required...

...but for viral vector core facilities producing a large number of viral preparations in parallel, there ...