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...When I started writing for the Addgene blog, I was focused on writing about new scientific techniques...the workspace. The color and size of icons are adjustable. New icons can be requested, although there’s...

...hours of neuronal excitation or inhibition. This sustained activation period is beneficial for use in therapeutic...permanent intracranial implant to be surgically installed to allow for light delivery. Chemogenetics poses...

...of fast bursts, as well as slow up/down voltage states in deep tissue, in vivo. Two of their constructs...Find JEDI-2p plasmids here!  Liu, Zhuohe et al. Sustained deep-tissue voltage recording using a fast indicator...

...Optogenetic Tools Like pDawn  Making cassava plants resistant to cassava mosaic virus Dr. Fiona Robertson, University... order to manage insect pests and to support sustainable agriculture. For this project the expression ...

... evolution took over (with a little mutagenic assistance). Quicker CAST activity allowed certain mutated... the National Academy of Sciences of the United States of America, 114(35), E7358–E7366. https://doi.org...

...open doors to fresh insights and broaden your understanding, ultimately enhancing your project's potential...museums can be excellent methods to detach from the constant stream of thoughts related to lab research and...

...SunTag is expressed at very low levels due to poor stability of the GCN4 scaffold. To increase expression, ...sequence to increase its alpha-helical structure and stability, creating v4 SunTag. Since the v4 system does ...

...Page! The start of the conference After setting up the Addgene booth on Thursday evening, I stayed to see...

...that I love explaining things to people. When I started my graduate work at Harvard, I recognized that ...are transferable to many different fields. If you start thinking about preparing yourself for a career while...

...antibiotic resistance. Next, consider the expression level of your construct: would you like to stay as close...

...aptamers and MS2-binding fluorescent proteins were the state of the art in RNA imaging in vivo. These new “Riboglow... the brightness. Arne Fabritius and colleagues started with the FP mNeptune684 (originally described by...

...about the paper recently published by her lab and Mustafa Khammash's lab at ETH Zürich. “Instead of a graphical... it!Disclaimer: I had to cut it very abruptly to stay within the time limit. pic.twitter.com/j6rhCCby2m...

...repository are used to produce CRISPR tools, we’re starting the Viral Service with lentiviral preps for the...transductions, and overviews for beginners just starting work with virus. You can find all of our viral...

...can be ordered as oligonucleotides and should be stable in the cell. gDNA guides will also need to be transfected...-stranded breaks, with either blunt (Cas9) or staggered (Cpf1) cleavage. NgAgo takes a different approach...

...of studying the effects of endogenous looping on distal promoters and enhancers, or have attempted to engineer...these LADL Anchors to genomic targets, such as a distal promoter and an enhancer. What makes LADL unique...

...high contrast between its resting state and its calcium-bound state, which was achieved by lowering the...

...Now with the DAP array, it is possible to use the state-of-art base editor, prime editor, or any CRISPR-...CRISPR-based gene editors, for correcting mutations or installing functional genes to study and potentially treat...

...distribution. You can also use these tips as a starting point when you need to amplify a library for your...Typically they’ll be in a file in FASTQ format, a standard format for storing NGS reads and their corresponding...

... demand for the related plasmid. Under such circumstances, we would observe this same correlation, but...ssrn.com/author=1232466.     Neil Thompson is an Assistant Professor of Innovation and Strategy at the MIT...

...including the product origin; purity, mechanism, and stability; and sequence of biologic product, mode of administration...drug’s immunogenicity in-vitro helps researchers understand a drug’s safety and efficacy in vivo.  ADAs ...