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Showing: 721 - 740 of 842 results

  1. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ...resulting in GFP signal. With the addition of standards, the assay is semi-quantitative and can approximate...plasmids and suitability of various antibiotic resistance markers, promoters, and inducible gene expression...

  2. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    Published
    April 6, 2017, 1:02 p.m.
    ...use electroporation, a technique that briefly destabilizes the cell’s outer membrane and allows the components...structures and processes and can lead to the better understanding of cell biology. We hope that these high-quality...

  3. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    Published
    Jan. 10, 2017, 3:37 p.m.
    ... effect different perturbations. Consider, for instance, the challenge of attempting to simultaneously...without making any functional changes to Cas9. For instance, when native (nuclease-competent) Cas9 interacts...

  5. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...vivo studies should always take DNA’s chromatin status into account (Fig 1a). The laboratory of Karmella...dCas9 expression and contain different antibiotic resistance markers. New CRISPR pooled library from Keith...

  7. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    Published
    Nov. 7, 2017, 1:59 p.m.
    ...that treat disease at the molecular level. For instance, if a disease is caused by a mutation in a single...developing AAV-based therapies. Furthermore, deeper understanding of disease pathogenesis will enable robust therapeutic...

  8. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    Published
    April 28, 2016, 2:30 p.m.
    ...considered "Cre-On" meaning that your gene of interest starts in the inverse/antisense "off" position and is ...employed for "Cre-off" (i.e. your gene of interest starts in the sense orientation and Cre is used to flip...

  9. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    Published
    Oct. 12, 2017, 2:26 p.m.
    ...light up endogenous genes of interest to better understand normal protein function. However, CRISPR protein...introduced directly into a Drosophila S2 cell line stably expressing Cas9. This PCR toolkit offers C- and...

  10. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    Published
    June 25, 2024, 1:15 p.m.
    ...et al., 2024) have highlighted how chromatin substantially affects prime editing outcomes across many genomic...with PRIDICT/PRIDICT2.0, ePRIDICT can help you understand how chromatin might influence editing results...

  11. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    Published
    Dec. 7, 2020, 2:53 p.m.
    ...randomly, or at fixed genomic target sites.  This obstacle was recently surpassed with the discovery of CRISPR-transposons...genome engineering in bacteria, alongside more established tools such as Cas9-recombineering, CRISPRa/i,...

  13. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    Published
    Sept. 28, 2017, 2:01 p.m.
    ...CNS and PNS! What is the CREATE method?  CREATE stands for Cre Recombinase-based AAV Targeted Evolution...number of neurons overall (GFP+ cells that also stained for a neuronal marker). Check out tables 1 and ...

  14. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    Published
    Oct. 18, 2018, 12:37 p.m.
    ...is carefully designed primers. The majority of mistakes are due to incorrect primer sequences! Primers...transduction, and uses electron cryo-microscopy to understand receptor function.     Follow them on Twitter...

  15. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ... (MCS). Modules are assembled one at a time by standard restriction enzyme digestion followed by ligation...Goler JA, Arkin AP, et al. BglBricks: A flexible standard for biological part assembly. Journal of biological...

  16. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    Published
    March 20, 2014, 3:04 p.m.
    ... should not even be the real goal. We are all constantly negotiating our energy and attention. The goal...going away to a conference. Really. No one will starve while you are away a few days. Also, your children...

  17. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...fluorescently labeled piggyBac vectors for uniform and sustained expression of single or multiplexed gRNAs and ...includes a CRISPRi-based synthetic oscillator, bistable network, stripe pattern-forming incoherent feed-forward...

  18. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    Published
    Oct. 13, 2022, 1:45 p.m.
    ... T’s. Thus, it’s important that there are no “bystander bases” if you choose this system.  Fig....demonstrated that targeting dCas9 to transcriptional start sites was sufficient to repress transcription by...

  20. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ...tool for sharing ideas. This leads to everyday obstacles for many of us, such as finding time to read all...platforms (such as social networking, visual appeal, instant sharing across multiple other sites), I created...
Showing: 721 - 740 of 842 results