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  1. Plasmids 101: How to Name Your Plasmid in 3 Easy Steps

    Type
    Blog Post
    Published
    Feb. 13, 2014, 1 p.m.
    ...can usually derive: a) the bacterial antibiotic resistance, b) the promoter that drives the insert, and ...followed by its position (number) relative to the start Methionine (Met) followed by the one letter abbreviation...

  2. The Addgene Antibody Data Hub

    Type
    Blog Post
    Published
    Sept. 12, 2024, 1:15 p.m.
    ... your research, you've probably found yourself staring at a browser full of tabs, each open to a different...anti-Lhx6.1 antibody.    For a more detailed understanding of the experimental conditions in which the ...

  3. Plasmid Preps: Different Purity, Different Quantities, Different Uses

    Type
    Blog Post
    Published
    June 16, 2020, 1:25 p.m.
    ...several hundreds of nanograms or micrograms of starting material; Gibson assembly or Golden gate assembly...success.  In conclusion, it is important for you to establish in advance how you want to utilize your plasmid...

  4. Inntags: Innovative Protein Epitope Tagging

    Type
    Blog Post
    Published
    Nov. 10, 2015, 3:30 p.m.
    ...protein, inntags IT5 and IT6 are a great place to start. These tags are easy to use in multiple common applications...Nature methods (2015). PubMed PMID:26322837. 2. Stadler, Charlotte, et al. "Immunofluorescence and fluorescent-protein...

  5. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    Published
    Dec. 29, 2020, 2:15 p.m.
    ...plasmids in one cell, it might be that you can't stably maintain them in the cell. This blog post features...blogger Shravanti Suresh who studies CRISPR at Iowa State University detailed five methods for using CRISPR...

  6. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    Published
    Aug. 28, 2018, 12:04 p.m.
    ...microscope called the ‘foldscope’ (Dr. Prakash, Stanford University). Right there on the floor outside ...Microbiology and Fluorescent Protein Resources and get started!  I learned a lot about creative ways to do science...

  8. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    Published
    June 17, 2014, 7:57 p.m.
    ... a couple of tips: First, she recommends using standard BsaI in place of the high fidelity version (despite...investigating the issue and hopes to have a better understanding of the phenomenon soon.  Rest assured, as the...

  9. Why Do I Need an MTA Anyway?

    Type
    Blog Post
    Published
    May 1, 2014, 8:23 p.m.
    ... We understand - you’re a busy scientist working hard on research, and you need the right experimental...Have a question? Our friendly and knowledgeable staff is here to help. Contact us at [email protected] or...

  10. Save Time and Money by Making Your Own Competent Cells

    Type
    Blog Post
    Published
    Jan. 28, 2014, 3:10 p.m.
    ...you're transforming a plasmid with ampicillin resistance.  After a plasmid is introduced to bacteria, ...culture without antibiotics to give the antibiotic resistance gene time to be expressed.  However, you can ...

  11. Editor's Choice, September 2016

    Type
    Blog Post
    Published
    Oct. 7, 2016, 4 p.m.
    ...contributed by guest blogger Sean Stacey from Eastern Tennessee State University and provides a brief rundown...

  13. Inside Addgene's 10 Year Anniversary Gala

    Type
    Blog Post
    Published
    July 3, 2014, 3:48 p.m.
    ...ending the celebration. The party later moved to State Park, where I had a great (maybe too great!) time...last 10 years @Addgene Check out our impressive stats from 10 Years of Plasmid Sharing Or read more about...

  14. Celebrating Accomplishments in the Lab

    Type
    Blog Post
    Published
    May 8, 2014, 3:07 p.m.
    ... at our next group meeting and see if we should start celebrating papers and grants too with pizza or ...Choosing a Research Project Advice for Scientists Starting a Lab ...

  15. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    Published
    Nov. 19, 2013, 2:37 p.m.
    ...desired targeting homology—in frame, of course—and start tagging! Yeast-optimized fluorophores for imaging...performance in categories such as brightness, stability, and disruption of the tagged protein. Based on...

  16. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    Published
    June 2, 2022, 2:15 p.m.
    ...back, I accepted that day and was excited for the start of my co-op in January. This excitement did not ...resources on the Addgene blog A Guide to Getting Started in Undergrad Research Tips for Writing a Good Cover...

  18. 2023 and 2024: Reflections and Looking Ahead

    Type
    Blog Post
    Published
    Jan. 23, 2024, 2:15 p.m.
    ...With renewed energy and optimism that comes at the start of every new year, it is a wonderful opportunity...for-profit companies, while making use of our nonprofit status to achieve mission-driven goals of accelerating...
Showing: 661 - 680 of 842 results