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Addgene
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  1. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...bbdone with our bbduk process.    At first, to make sure we understood the concept of channels and implemented...

  3. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    ...gene! STEP 2: Generate an Expression Clone When making the expression clone, it is important to choose...

  6. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ... than engineering an entirely new protein. This makes it much cheaper to use Cas9 activators. This larger...

  7. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...gRNA targets in that region. The CRISPR Software Matchmaker can be used to determine the pros and cons of...

  8. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ... generally ensure cell line identity and purity making it easier for comparable studies to be performed...

  10. Plasmids 101: Control Plasmids

    Type
    Blog Post
    Published
    April 9, 2015, 3:29 p.m.
    ...reproducibility controls". Biological replicates are what makes up your sample size (aka your n value)" and should...

  12. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ...exposed side chain that can be easily manipulated to make reactive species without affecting the avidin binding...

  15. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    Published
    June 27, 2024, 1:15 p.m.
    ...gating uses the same color for different markers, making it easy to gate on the negative cell population...

  16. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    .... Li, K et al. PLoS ONE 9(8); (2014). PubMed. Making designer mutants in model organisms. Peng, Y. et...

  17. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ...iGECI and NIR-GECO2G, and its small size (~1500 bp) makes iGECInano suitable for AAV packaging.    Find iGECInano...

  19. Evolution of Lab Techniques

    Type
    Blog Post
    Published
    June 21, 2016, 2:30 p.m.
    ...popular genome-engineering tool, CRISPR, may one day make techniques used to generate transgenic mouse lines...
Showing: 661 - 680 of 696 results