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  2. 5 Great Apps for Lab Life

    Type
    Blog Post
    Published
    Sept. 22, 2016, 2:30 p.m.
    ...technique, I try to look for a resource to help me. I’m still waiting for a mass spectrometry app. I digress. ...one convenient resource. I can safely say that I still have the single and triple abbreviations of the ...

  3. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    Published
    April 4, 2023, 1 p.m.
    ...either fission or budding, which is the primary distinction between two of the most frequently used yeast...highly efficient at protein secretion, making harvesting recombinant proteins a cinch. This is because...

  4. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    Published
    Aug. 23, 2022, 1:15 p.m.
    ...misinformation about sexual and reproductive health, as the stigma around sexual and reproductive health issues creates...never forgot about all the anxious, whispered questions from friends and classmates back in high school...

  6. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    Published
    March 14, 2019, 1:54 p.m.
    ...2014), but scientists have yet to decipher the mechanistic aspects behind these improvements. What have..."Enhanced liver gene transfer and evasion of preexisting humoral immunity with exosome-enveloped AAV vectors...

  7. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    Published
    Oct. 9, 2014, 3 p.m.
    ...spectra differ sufficiently for them to be imaged in distinct fluorescent channels on the microscope. With the... to choose fluorescent proteins that can be distinguished from one another and that can be imaged with...

  8. Fluorescent Proteins 101: Luciferase

    Type
    Blog Post
    Published
    June 24, 2014, 3:59 p.m.
    ...luciferase-containing plasmid is most often used to investigate the effect of regulatory elements, such as promoters...Pathways Known cellular signaling pathways can be investigated in a high-throughput screen using luciferase...

  9. Advice for Scientists Starting a Lab

    Type
    Blog Post
    Published
    April 29, 2014, 1:43 p.m.
    ...available from your institution, your department, and online. The Howard Hughes Medical Institute (HHMI) offers...

  11. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    Published
    May 9, 2017, 2:12 p.m.
    ... a jigsaw puzzle, the oligos were designed with sticky ends that only anneal together in one direction...plasmids available from Addgene. If you have any questions or thoughts about Cpf1 multiplexing, leave them...

  12. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    Published
    July 25, 2023, 1 p.m.
    ...making virus’ or what gag and pol stand for, we demystify all components of viral vectors here! Need Virus...into the host genome (more on this below), it’s still used to deliver gene expression constructs. Sandwiched...

  13. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    Published
    June 15, 2023, 1:15 p.m.
    ...producing the proteins – are not, and many of the existing GCE tools are not very robust. When using GCE,...work. Plus, keep an eye on our blog – we’ll be posting more posts on the various protocols and helping...

  14. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    Published
    March 21, 2023, 1:15 p.m.
    ...Image courtesy of National Human Genome Research Institute. Its genome, however, isn’t enough to ensure...their low immunogenicity, which means they do not stimulate a strong immune response when introduced into ...

  15. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    Published
    Aug. 6, 2024, 1:15 p.m.
    ...prototypical gamma-retrovirus.    Frequently asked questions about γ-retroviral vectors How do engineered γ-retroviruses...post‐transcriptional regulatory element; sequence that stimulates the expression of transgenes via increased nuclear...

  16. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    Published
    June 6, 2024, 1:50 p.m.
    ...application. These controls will allow you to distinguish real results from background noise or nonspecific...positive and negative cells. This can be achieved by adjusting your voltage using your single color controls ...

  18. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    Published
    Jan. 18, 2018, 2:34 p.m.
    ...promoters, are always active and thus referred to as constitutive promoters. Others are only active under specific...complexed with cAMP activator protein (CAP) can also stimulate AraC binding to I1 and I2 sites. Supplementing...

  19. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    Published
    Sept. 15, 2020, 1:15 p.m.
    ...to identify cells that took up the vector and distinguish between plasmids that contain the gRNA (white...Walkowski.   Restriction digest Another way to distinguish between colonies containing plasmids with inserts...

  20. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    Published
    May 26, 2020, 1:15 p.m.
    ..., integrated reporter for assaying CRISPR/Cas-stimulated HDR activity) The Madison lab created SRIRACCHA...integrated surrogate reporter for assaying CRISPR/Cas9-stimulated homology-directed repair. Journal of Biological...
Showing: 641 - 660 of 832 results