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...LitSuggest Tool Following the “rabbit hole” of related cell lines when annotating a cell line Community...whether a cell line is known to be genetically manipulated, its anatomical origin, if cell line has ever...

... left four years later with a master’s. At the time my degree felt like a consolation prize, but in time...

...bacteria, yeast, mammalian, etc.). To individually isolate a protein, there must be a special feature about...S-Transferase (GST) is a 26 kDa tag which can be isolated with glutathione resin. Like MBP, it can be removed...

... data, and I was given a fantastic spreadsheet template to build out a report on. I was able to understand...cultivates trust between coworkers and that trust then translated into this incredible experience.  Of course, ...

...may be most effective. Once drug candidates are isolated, they can be validated for specificity to their...before time and money gets put into them in those later stages. Target identification Not all diseases ...

...selectable marker. Each insertion line can then be isolated and screened. Depending on the marker, traps can...require the trapping of endogenous enhancers to stimulate the expression of the reporter. The system is ...

...proteins are also involved in the transcriptional regulation of the AAV p40 promoter which drives Cap expression...promoter. Alternative splicing and differing translational start sites generate the three VP proteins. ...

...inactivation, protein degradation, and/or the use of chelating agents that can be introduced in the purification...time. Tris buffer controls the pH, while the EDTA chelates any divalent cations like Mg2+, preventing the...

...person, and helps improve your interpersonal relationships with people you’re collaborating with remotely...external collaborations Browse our science career related blog posts ...

...use of viruses is also important for social and regulatory reasons. 1.) The transposon consists of a mirrored...your gene-of-interest, either constitutively or regulated by doxycycline. The Sleeping Beauty transposase...

...backbones) are frequently used in molecular biology to isolate, multiply, or express the insert they carry in ... system is great for overexpressing YGOI in an isolated, controlled environment. In most cases bacterial...

...which stands for Multiplexed Overexpression of Regulatory Factors. The Feng Zhang lab developed this library...Element. This library consists of broad host range regulatory sequences ready to be assembled with other elements...

...viral vector repository with a nice cup of hot chocolate is absolutely delightful. Grab a hot beverage ...forget to use the link below to keep up with the latest in Addgene's viral vector services and resources...

... projects studying developmentally programmed regulation of DNA replication and gene transcription during... useful and productive, forming a different constellation of interests and skills than what fits for applied...

...sorbitol is already a common excipient in AAV formulation buffers, this sugar may be a more attractive ...Charlotte A., Michael Hollinshead, and Amin Hajitou. "Clathrin-mediated endocytosis and subsequent endo-lysosomal...

...scheduling Diverse opportunities to do science related jobs that require no bench work Better resources... important first step. Developing mentoring relationships, especially with peer mentors, to discuss your...

...single mRNA translation in U2OS cells. HA is small, only 9 amino acids, thus it is translated quickly by...

...genomes can be transcribed into mRNA, directly translated into proteins, or, in the case of retroviruses...which is then transcribed into mRNA before being translated into proteins. RNA viruses replicate their genomes...

...even by introducing a person that the audience can relate to. It doesn’t need to be anything fancy; it just... anthropomorphized animals, or even seemingly unrelated stories that they then tuck the science into. ...

... focus of the NABOR platform will be neuroscience reagents and data, the platform can be expanded for ...