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...proteins are also involved in the transcriptional regulation of the AAV p40 promoter which drives Cap expression...promoter. Alternative splicing and differing translational start sites generate the three VP proteins. ...

...endothelial cells via an injection into the systemic circulation. In Old World monkeys it actually targets neurons...forget to use the link below to keep up with the latest in Addgene's viral vector services and resources...

...inactivation, protein degradation, and/or the use of chelating agents that can be introduced in the purification...time. Tris buffer controls the pH, while the EDTA chelates any divalent cations like Mg2+, preventing the...

...person, and helps improve your interpersonal relationships with people you’re collaborating with remotely...external collaborations Browse our science career related blog posts ...

...researchers represent a minority of the world’s population, we account for an estimated 2% of global plastic...it has to be packaging (no coated tissue culture plates, for example). Check the link for more details ...

...cultural importance of these animals to human populations around the world. Here at Addgene, we love camelids...living cells or tissues. sdAbs can also be used to manipulate cellular functions, like in this early example...

...be located. By easily using BLAST to check the translated amino acid sequence from within Snapgene, we ...size of the plasmid and the insert. By using the “Simulate agarose gel” option with the desktop version of...

... to discover your insights on a topic. Beyond collating other’s data, you can even include your own data...patients was published, Zika researcher Christa Osuna collated Zika case studies, making a meta analysis available...

... part of the job, but Caitlin doesn’t work in isolation. “A lot of what we do is work with students who...theses, and occasionally posting something science-related on Facebook. “I think this helped me say with confidence...

...identified proteins and enzymes. These compounds will later get tested for their potential agricultural and ...agriculture. For this project the expression of isolated bacterial proteins can be achieved using a bacterial...

...confidentiality where it is appropriate. This grows our relationship with our community members. We also find that...Mission Meet the Addgene Leadership Team Get the Latest News from Addgene ...

...use of viruses is also important for social and regulatory reasons. 1.) The transposon consists of a mirrored...your gene-of-interest, either constitutively or regulated by doxycycline. The Sleeping Beauty transposase...

...backbones) are frequently used in molecular biology to isolate, multiply, or express the insert they carry in ... system is great for overexpressing YGOI in an isolated, controlled environment. In most cases bacterial...

...which stands for Multiplexed Overexpression of Regulatory Factors. The Feng Zhang lab developed this library...Element. This library consists of broad host range regulatory sequences ready to be assembled with other elements...

...viral vector repository with a nice cup of hot chocolate is absolutely delightful. Grab a hot beverage ...forget to use the link below to keep up with the latest in Addgene's viral vector services and resources...

...genomes can be transcribed into mRNA, directly translated into proteins, or, in the case of retroviruses...which is then transcribed into mRNA before being translated into proteins. RNA viruses replicate their genomes...

...even by introducing a person that the audience can relate to. It doesn’t need to be anything fancy; it just... anthropomorphized animals, or even seemingly unrelated stories that they then tuck the science into. ...

...sorbitol is already a common excipient in AAV formulation buffers, this sugar may be a more attractive ...Charlotte A., Michael Hollinshead, and Amin Hajitou. "Clathrin-mediated endocytosis and subsequent endo-lysosomal...

... focus of the NABOR platform will be neuroscience reagents and data, the platform can be expanded for ...

...single mRNA translation in U2OS cells. HA is small, only 9 amino acids, thus it is translated quickly by...