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TypeBlog PostPublishedJan. 14, 2021, 1:30 p.m....showcase it all! View this post on Instagram A post shared...able to #DeckTheLab and continue the tradition we started last year: The Christmas Decoration Competition.... 🙌 🥂 🎄 🎉 😷 Remember wear a mask,↔️social distance, 💓show gratitude,☎️ call up an old friend. Remind...
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Interview with Bart Newland, Addgene’s Newest Board Member
TypeBlog PostPublishedAug. 3, 2021, 1:15 p.m....Newland is General Counsel at Atalanta, an early-stage biotech company that is developing RNAi therapeutics...transition now to being general counsel of a small start-up. For the last 20 years, I was a patent counsel...different jobs. Being general counsel at a small start-up is very interesting. It’s very varied. There’... -
AAV Packaged on Request is Here!
TypeBlog PostPublishedJan. 10, 2025, 1:01 p.m....our new AAV Packaged on Request webpage to get started with your browsing and requesting. If you’re curious...easier to go from selection to viral vector. To start the AAV packaged on request process, simply navigate...placed, we’ll facilitate the necessary MTA, then start production and quality control. Our packaged on... -
Podcast: Interview with Addgene's Founders
TypeBlog PostPublishedApril 13, 2018, 12:51 p.m....you’ve ever been curious about what it takes to start your own nonprofit, this is the episode for you....the ground Chapter 3 - Addgene as a leader in standardized tech transfer and the adoption of the UBMTA ...Developing Addgene as a modern e-commerce company and establishing credibility Chapter 5 - Realizing that Addgene... -
A Scientist's Guide to Ebola
TypeBlog PostPublishedOct. 16, 2014, 3:01 p.m....Dallas was the first to be infected in the United States while caring for a patient, despite wearing protective...Recommended Reading For those looking to delve deeper or stay tuned, there are plenty of online resources available...available. I recommend a few: -The recently established Ebola Genome Portal at the University of California... -
Addgene's Website Update
TypeBlog PostPublishedApril 8, 2024, 2:15 p.m....Starting today, you may notice some changes to our website! Part of sharing science and making access...plasmids from a specific paper. If you’re ready to start a deposit, simply click on “Deposit Materials” and...may find one of your most frequently used buttons stands out better on the page, or that the text is just... -
A Conference By Postdocs For Postdocs: Future of Research
TypeBlog PostPublishedSept. 2, 2014, 3:05 p.m.... in some form? There is a lot of talk about the state of scientist training in the US and around the world...postdoc-driven biotechnology clubs such as Penn State, Berkeley and Harvard. Students also partner with...their own hands, there are some easy ways to get started. I am happy to work with any group of trainees ... -
Stem Cell Models for Disease & Open Science: Interview with Darrell Kotton
TypeBlog PostPublishedJuly 7, 2017, 1:32 p.m....Goals of the Center for Regenerative Meidince - Understanding and developing means to treat disease through...surfactant producing cells that can be used to better understand the biology of certain lung diseases. Chapter...Outro - remember to step back and ask, "Why did I start doing this in the first place?" Many thanks to ... -
Quick, Versatile Plant Transgenesis with GreenGate Plasmids
TypeBlog PostPublishedJan. 23, 2014, 3:23 p.m....interest, C-terminal tags, plant terminators and resistance cassettes for selection of transgenic plants.... available at Addgene, also gives the option to stack several expression cassettes onto a single T-DNA...that they cut at arbitrary sequences a defined distance away from the recognition site. In other words... -
Top 10 Open Science Developments of 2013
TypeBlog PostPublishedDec. 19, 2013, 3:46 p.m.... after all, with each generation of scientists standing on the shoulders of their colleagues and all those...Also in June, the G8 Science Ministers issued a statement after a meeting in London on the global science... as much about open access as it does about the state of peer review. 8) That same issue of Science... -
Six Spooky Science Stories and Halloween at Addgene
TypeBlog PostPublishedOct. 31, 2019, 1:06 p.m....infects a snail’s eyestalks. Once ingested by the snail, the larvae reach the eyestalk and transform them...fish (Lutjanus guttatus) by a parasitic isopod (Crustacea: Isopoda)." Copeia 1983.3 (1983): 813-816. Gilbert... -
Seeing Red: Simple GFP Photoconversion
TypeBlog PostPublishedAug. 4, 2015, 2:30 p.m.... that purified GFP could be imaged in the green state after excitation at 488 nm, photoconverted with ...with 405 nm light, and then captured in the red state after excitation at 561 nm. This imaging process worked...Hazelwood KL, Davidson MW, Looger LL. A bright and photostable photoconvertible fluorescent protein. Nat Methods... -
Scientific Sharing in the Time of COVID-19: Databases and Resources
TypeBlog PostPublishedMay 12, 2020, 1:15 p.m....sharing as the scientific community strives to understand COVID-19. While the specific motivations vary...scientific community can make progress towards understanding this disease. Figure 1: Scientists are...investigate COVID-19, and once universities reopen and restart other research, scientific sharing will remain ... -
SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture
TypeBlog PostPublishedAug. 13, 2014, 3:49 p.m....circulating tumor cells, we found that the binding stability really mattered for efficient capture. We wanted...Andrews discovered the domain. It is small, highly stable and has this amazing chemistry locking the domain...(CTCs) is going to have a big impact on our understanding and treatment of cancer. It is clear that one... -
The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins
TypeBlog PostPublishedJuly 29, 2014, 6:16 p.m....cell biology, Michael Davidson’s lab at Florida State University has been the go-to place. In 2012, his...every fluorescent protein under the sun, from the standard oldies but goodies (e.g., EGFP and YFP) to the...Democrat in a story about his life’s work and the stage IV lung cancer that has now prompted him to deposit... -
CRISPR 101: Cytosine and Adenine Base Editors
TypeBlog PostPublishedFeb. 13, 2025, 2:15 p.m....specified by the Cas protein These elements were the starting point towards the development of the first cytosine...divisions, indicating that this method produces stable edits. However, this system is also subject to ...cytosines in the base editing window, reducing bystander editing (Wu et al., 2025). Adenine base editors...DNA before the edit is made. Using ABE7.10 as a starting point, Gaudelli evolved the base editor into 40... -
Cellosaurus: A Cell Line Information Database
TypeBlog PostPublishedFeb. 21, 2023, 2:15 p.m....ever been the target of an ‘-omics’ study or is resistant to specific compounds, links to publication references...down the hall who got it from some technician downstairs who got it from who knows where), now is as good...surprising, and important, findings being shared. For instance, recently Cellosaurus received a report that HT... -
Five Questions to Ask If You're Deciding Between a Master's or PhD Program
TypeBlog PostPublishedFeb. 16, 2021, 2:15 p.m....work. 5. Do you enjoy bench work? It’s easier to stay at the bench longer (or for your whole career) with...often technical career tracks for PhDs who want to stay at the bench. So if you enjoy wielding a pipette...hopefully the questions in this blog post can help you start to figure out if a master’s is a good fit with your... -
CUT&RUN: An Improved Method for Studying Protein-DNA Interactions
TypeBlog PostPublishedFeb. 13, 2018, 2:51 p.m....during an initial fixation step. This serves to stabilize DNA-protein interactions for subsequent sheering... of the limitations of ChIP-seq. CUT&RUN, which stands for ‘Cleavage Under Targets and Release Using Nuclease...likely that this technique will quickly become a standard method alongside ChIP. Many thanks to our guest... -
ReFigure: Save Scientific Figures into Dashboards and Share Your Insights
TypeBlog PostPublishedDec. 13, 2017, 3:20 p.m.... For instance, long before a study with multiple patients was published, Zika researcher Christa Osuna... and good for science ReFigures are published instantly and are editable by the author at any time. Insights...