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Addgene
Showing: 581 - 600 of 832 results

  1. Addgene Stands with the Scientific Community

    Type
    Blog Post
    Published
    April 23, 2025, 1:30 p.m.
    ...Addgene is uniquely positioned to help research institutions maximize their increasingly limited resources...investment isn't lost. Universities and research institutions can turn to Addgene as a trusted partner for...

  2. What's New in CRISPR - May 2019

    Type
    Blog Post
    Published
    May 28, 2019, 12:49 p.m.
    ...new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools... was developed by Rebecca Shapiro’s lab. After testing dCas9 fusions with transcriptional repressors, ...

  3. Your Top Requested Plasmid in 2015!

    Type
    Blog Post
    Published
    Dec. 30, 2015, 3:30 p.m.
    ...lab at the Broad Institute and was designed and cloned by Neville Sanjana. This fantastic research tool ...

  4. Inntags: Innovative Protein Epitope Tagging

    Type
    Blog Post
    Published
    Nov. 10, 2015, 3:30 p.m.
    ...domains from non-vertebrate systems. Preliminary testing indicated that inntags IT5, IT6, and IT10 expressed...and the chaperone Ydj1, both of which display sophisticated interactions with multiple other proteins. Again...

  5. A Conference By Postdocs For Postdocs: Future of Research

    Type
    Blog Post
    Published
    Sept. 2, 2014, 3:05 p.m.
    ...Bourne has some radical (and some not so radical) suggestions for defining the problem and for finding solutions...don't have the resources to do enough. There are still university research departments that have no formal...

  6. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    Published
    March 11, 2014, 5:55 p.m.
    ...Structural details All this, and scientists are still working out important details in their understanding... tool. A week later, researchers at the Broad Institute and MIT published another report showing Cas9'...

  8. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    Published
    March 21, 2019, 12:47 p.m.
    ...new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools...CRISPR-based marker excision method for marking distinct cell clones developed by the Michalis Averof lab...

  9. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    Published
    June 17, 2014, 7:57 p.m.
    ...specificity engineering. “The RNA binding domain is interesting because by changing certain amino acids you can...lose their solubility. Her lab is currently investigating the issue and hopes to have a better understanding...

  10. Developing Transferable Skills During Science Training

    Type
    Blog Post
    Published
    June 3, 2014, 2:14 p.m.
    ...project involving multiple scientists, create and stick to timelines Be a mentor in a formal program Start...Collaboration and teamwork One of the defining characteristics of nonacademic science careers is that a team...

  12. Let There Be LITE Plasmids

    Type
    Blog Post
    Published
    Nov. 21, 2013, 3:56 p.m.
    ...anchors for genome engineering applications was still very new. Now, Konermann and her colleagues including...transcription in as little as 30 minutes after light stimulation. Under those optogenetic controls, gene expression...

  13. Top Requested AAV of 2017: pmSyn1-EBFP-CRE

    Type
    Blog Post
    Published
    Jan. 17, 2018, 2:57 p.m.
    ...our viral service in late 2016 and requests are still pouring in! While our lentiviral service is going... many more tips and ideas on how to use these fantastic tools in your experiments.  Many thanks to Hongkui...

  14. What's New in CRISPR - December 2019

    Type
    Blog Post
    Published
    Dec. 17, 2019, 2:37 p.m.
    ...new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools...of ADAR2 into a cytidine deaminase. This editor still retains A-to-I editing. This system is called RNA...

  15. Reflections and Looking Ahead

    Type
    Blog Post
    Published
    Feb. 25, 2025, 2:15 p.m.
    ...quality control. Through our partnership with the Institute for Protein Innovation (IPI), we expanded a growing...repository can now be packaged on demand in five distinct serotypes, offering you unparalleled flexibility...

  18. X-CHIME: Context Dependent Germline Knockout in Immune Cells

    Type
    Blog Post
    Published
    Jan. 16, 2024, 6:13 p.m.
    ...CHimeric IMmune Editing system) to enable the constitutive, ubiquitous knockout of single genes in immune...To demonstrate the power of these systems to investigate in vivo mechanisms, we targeted two phosphatases...

  19. Searchable and Sortable gRNAs for Your Next CRISPR Experiment

    Type
    Blog Post
    Published
    May 17, 2016, 2:30 p.m.
    ...save you the time that would be spent making and testing entirely new gRNA designs. You can now easily find...variations (e.g. SNPs) exist. Additionally, consider testing multiple gRNAs targeted to the same locus to ensure...

  20. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    Published
    Aug. 4, 2015, 2:30 p.m.
    ... FPs since its emission wavelength (612 nm) is distinct from other commonly used FPs, and GFP does not...localization that have been encountered by some investigators when using forms of mEos." The ability to repurpose...
Showing: 581 - 600 of 832 results