We narrowed to 621 results for: Dos
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TypeBlog PostPublishedMay 5, 2020, 1:15 p.m....utmost priority for countries around the world and to do so, the WHO recommends one strategy: testing, tracking...Servellita V, Singh J, Miao X, Streithorst JA, Granados A, Sotomayor-Gonzalez A, Zorn K, Gopez A, Hsu ...
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CRISPR 101: Any Base Transversion Editors
TypeBlog PostPublishedApril 1, 2025, 1:15 p.m....tested with AYBEv3!). Although AYBE, AXBE, and ACBE do not approach the high product purity of CBEs and ... -
Harnessing Bacterial Toxins for Allelic Exchange
TypeBlog PostPublishedAug. 15, 2019, 12:30 p.m....negative selection markers for allelic exchange. To do this, we performed the following modifications to... -
New Optogenetic Tools for Cytoskeleton and Membrane Control
TypeBlog PostPublishedSept. 28, 2023, 1:15 p.m....processes in different cell types. One great way to do this is to use CRY2-CIB1 clustering. Fuse either ...provided CIB constructs for targeting Golgi, ER, and endosome membranes) (Figure 4). The authors used SuperPLD... -
Using Phosphoserine to Study Protein Phosphorylation
TypeBlog PostPublishedJune 23, 2016, 2:30 p.m...., phosphomimetic mutations on the activation loop do not fully activate MEK1. In fact, replacing just ... -
Evolution of Lab Techniques
TypeBlog PostPublishedJune 21, 2016, 2:30 p.m....comparison. These next-generation sequencing techniques do not typically rely on Sanger sequencing but instead... -
Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods
TypeBlog PostPublishedMarch 1, 2016, 3:30 p.m.... by restriction enzyme cloning, you would have to do it in two steps, and a scar would likely remain between... -
Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons
TypeBlog PostPublishedApril 24, 2015, 2:39 p.m....default state. In contrast, Brainbow-3.1 and -3.2 do not display default fluorescence due to the addition... -
Technologies Enabled by NanoLuc® Luciferase
TypeBlog PostPublishedFeb. 8, 2018, 12:17 p.m....near optimal wavelengths (Fluc emits at 610 nm) and do not require external light sources. Unfortunately... -
Plasmids 101: Repressible Promoters
TypeBlog PostPublishedDec. 20, 2022, 2:15 p.m....systems listed above are orthogonal, meaning that they do not affect each other. For example, the GAL4 transcription... -
Viral Vectors 101: AAV Variables That Matter
TypeBlog PostPublishedJune 1, 2023, 1:15 p.m....success, we find it is usually faster and easier to do the validation first. AAV-mediated gene expression... -
RNA Interference in Plant Biology: New Tools for an Old Favorite
TypeBlog PostPublishedOct. 27, 2020, 1:15 p.m....you’ve introduced your RNAi into your organism, how do you identify the actively silenced ones? Here, I’... -
Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering
TypeBlog PostPublishedDec. 15, 2016, 3:57 p.m....recombinant clones are generated. A simple way to do this is to express lambda red genes from a plasmid... -
Which Fluorescence Microscopy Technique is Best for Me?
TypeBlog PostPublishedOct. 10, 2017, 1:57 p.m....μm) tissue sections and 3D cultures These samples do not necessarily require optical sectioning, but open...while also avoiding the toxic effects of high light doses to the cells. Thin static samples Ex: Fixed monolayers...out of focus light. However, they also continually dose the sample from top to bottom with excitation light... -
Typing CRISPR Systems
TypeBlog PostPublishedMarch 18, 2025, 1:15 p.m....adaptive immune functions that other CRISPR types do, due to a lack of canonical CRISPR features (Pinilla-Redondo... -
Delivery Methods for Generating iPSCs
TypeBlog PostPublishedApril 17, 2018, 1:37 p.m.... a new or experienced reprogrammer? Which methods do you have experience using? Let us know about your...multiple transfection makes it difficult to control the dose of plasmid the cells receive over the whole reprogramming... -
27 Hot Plasmids from 2016
TypeBlog PostPublishedDec. 22, 2016, 3:03 p.m.... significant medical interest, however efforts to do so have been hindered by a lack of tools, compounds...target soluble kinase domains, but these domains often do not express well in E. coli without their partner...cells. The mutant cells were treated with WNV at a dose and duration sufficient to kill all control cells...and not specific loci. To overcome this flaw, the Zoldos lab has developed CRISPR tools for epigenome editing...and their effects on gene regulation. Professor Zoldos and colleagues from the Department of the Biology...deletion efficiency was dependent upon 1) Cas9 and gRNA dose (both of which can be modulated using the different... -
15 Hot Plasmids from 2017
TypeBlog PostPublishedJan. 9, 2018, 3:02 p.m....Agudelo et al. prevent ouabain binding to ATP1A1 but do not compromise the ion pump’s function or cause cellular... -
22 Hot Plasmid Technologies from 2014
TypeBlog PostPublishedJan. 6, 2015, 5:21 p.m....authors the “Clonetegration”, is quick and easy to do. Clonetegration could become a “valuable technique... -
Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants
TypeBlog PostPublishedJune 22, 2021, 1:15 p.m.... Difference in neutralization after one dose and two doses of the mRNA vaccines While there have been.... Antibodies from individuals who received both doses of the Moderna or Pfizer vaccine could neutralize...from individuals who received either one or two doses of the Pfizer or Modern vaccine. This sera was used...emphasizing the importance of the second vaccine dose in mounting a robust immune response. The Balazs...