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We narrowed to 628 results for: Dos

Showing: 581 - 600 of 628 results

  2. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    Published
    Aug. 2, 2016, 2:30 p.m.
    ... extension can usually ensure that the 3’-base(s) do not form secondary structures. The introduction (...

  3. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ...caption courtesy of Challis et al., 2022. Where do they come from? Systemic capsids are typically derived...efficacy can vary depending on species. LY6A and LY6C1 do not have known homologs in NHPs, and capsids that...mouse strains). AAV-PHP.eC, AAV9-X1.1 and AAV1-X1 do not display strain-specific tropism and can be used...2023). High viral titer High vector genome (vg) doses are recommended for all systemic capsid applications... AAV-PHP.eB is typically administered at a total dose between 1 x 1011 and 5 x 1011 vg per animal (i.e...mL titer), while AAV-PHP.S is typically used at a dose between 3 x 1011 and 1 x 1012 vg*. Most systemic...capsids are easy to produce in high titer, but higher doses (>1 x 1012 vg total in mice) may increase the chances...

  4. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ...utmost priority for countries around the world and to do so, the WHO recommends one strategy: testing, tracking...Servellita V, Singh J, Miao X, Streithorst JA, Granados A, Sotomayor-Gonzalez A, Zorn K, Gopez A, Hsu ...

  7. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ...processes in different cell types. One great way to do this is to use CRY2-CIB1 clustering. Fuse either ...provided CIB constructs for targeting Golgi, ER, and endosome membranes) (Figure 4). The authors used SuperPLD...

  8. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    Published
    Jan. 3, 2019, 1:35 p.m.
    ...your target base in the protospacer sequence. If you do not find a suitable ‘NGG’ PAM at your target genomic...

  10. Evolution of Lab Techniques

    Type
    Blog Post
    Published
    June 21, 2016, 2:30 p.m.
    ...comparison. These next-generation sequencing techniques do not typically rely on Sanger sequencing but instead...

  14. Exploring Applications of the Bioluminescent HiBiT Tag

    Type
    Blog Post
    Published
    Oct. 2, 2025, 1:15 p.m.
    ...surface epitopes for many proteins, but some proteins do not have such epitopes. These classical 'undruggable... to track endogenous activity of EGFR, including dose-dependent response to EGF (Promega, 2025). The value...diminished luminescence signal, associated with endosomal localization of EGFR. This method offered kinetic...

  15. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ...systems listed above are orthogonal, meaning that they do not affect each other. For example, the GAL4 transcription...

  19. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    Published
    Oct. 10, 2017, 1:57 p.m.
    ...μm) tissue sections and 3D cultures These samples do not necessarily require optical sectioning, but open...while also avoiding the toxic effects of high light doses to the cells. Thin static samples Ex: Fixed monolayers...out of focus light. However, they also continually dose the sample from top to bottom with excitation light...

  20. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ...adaptive immune functions that other CRISPR types do, due to a lack of canonical CRISPR features (Pinilla-Redondo...
Showing: 581 - 600 of 628 results