27 Hot Plasmids from 2016
Type
Blog Post
Published
Dec. 22, 2016, 3:03 p.m.
... significant medical interest, however efforts to do so have been hindered by a lack of tools, compounds...target soluble kinase domains, but these domains often do not express well in E. coli without their partner...cells. The mutant cells were treated with WNV at a dose and duration sufficient to kill all control cells...and not specific loci. To overcome this flaw, the Zoldos lab has developed CRISPR tools for epigenome editing...and their effects on gene regulation.
Professor Zoldos and colleagues from the Department of the Biology...deletion efficiency was dependent upon 1) Cas9 and gRNA dose (both of which can be modulated using the different...