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Addgene
Showing: 41 - 60 of 102 results

  1. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...the University of Pennsylvania Vector Core. Transfections are performed using the transfer plasmid, a ...primers targeting the transfer plasmid used in the transfection. PCR products are analyzed by gel electrophoresis...

  2. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    Published
    Nov. 5, 2024, 8:32 p.m.
    ...in mammalian cells is relatively quick. After transfection, HEK-293T cells start producing virus within...systems, it is standard to do only one passage (transfection). This differs from typical insect cell preparations...

  3. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...be produced through a single or dual-plasmid transfection, depending on whether the heavy and light chain...they’ve been separated onto two. You’ll first transfect your cells with your plasmid(s), and then wait...

  5. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ...packaging and host genome integration. After co-transfection of these plasmids, 293T cells released transgene-containing...work with primary cells or other difficult-to-transfect cells. Lentiviral barcoding libraries have been...

  6. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    Published
    March 27, 2018, 1:32 p.m.
    ...identical targeting vectors delivered through transfection of naked DNA. Other investigators who had been...then packaged into AAV virions via transient transfection of 293T cells with helpers, and used to infect...

  8. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...site. (c) The pCAG-EGxxFP target plasmid was co-transfected with pX330 plasmids containing sgRNA sequences...efficacy of your gRNAs is then a simple matter of transfecting HEK293T cells with your modified pCAG-EGXXFP...

  9. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ...the passage number of a culture the lower the transfection and transduction efficiencies. Cultures should... while others opt for a single harvest after transfecting with the transfer and packaging plasmids. A ...

  10. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ...as effective NIR fluorescence in transiently transfected live HeLa cells with no supply of exogenous BV...and after normalization to fluorescence of co-transfected EGFP. e Originally reported as a monomer16, IFP2.0...

  11. Our Most Popular Blog Posts from 2019

    Type
    Blog Post
    Published
    Dec. 31, 2019, 2:26 p.m.
    ...Transformation, Transduction, Bacterial Conjugation, and Transfection by Alyssa Cecchetelli The introduction of nucleic...

  13. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ...multimers may exhibit reduced transformation and transfection efficiency compared to the monomeric version... Hartl D, Rudolph C. Transgene expression of transfected supercoiled plasmid DNA concatemers in mammalian...

  14. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    Published
    Sept. 14, 2017, 12:40 p.m.
    ...Harrington et al tested GeoCas9’s editing skills by transfecting HEK293 cells with GeoCas9 or SpCas9 RNP’s and...

  19. Fluorescent Proteins 101: Luciferase

    Type
    Blog Post
    Published
    June 24, 2014, 3:59 p.m.
    ...luciferase containing a stop codon, which is then transfected into the desired cells. A hybrid mRNA transcript...
Showing: 41 - 60 of 102 results