...much for how bright you can make a reaction but for how sensitive you can make a reaction. NLuc requires...proteins to make better reporters and the second couples NLuc to fluorescent proteins to make better biosensors...interesting construct you wished to use, you could either make it yourself or you could “clone by phone”. One of...tactics for getting the plasmids he wanted. Addgene makes it so much easier to share your constructs from ... however NLuc’s emission peak is at 460 nm thus making it non-optimal for in vivo imaging. The solution...the fluorescent donor for a bioluminescent donor, making BRET probes. Here the bioluminescent donor is unaffected... fluorescent proteins has allowed researchers to make innovative tools not possible with other luciferases...

...genome is 1 in every 256 base-pairs of DNA sequence, making it very useful for genetic engineering approaches... and gene editing. How does the piggyBac™ system make it easier to generate transgenic cell lines? One...transgenic cell lines. The traditional method of making stable cell lines is to transfect, select, and ...negative impacts on fitness. One consideration when making a transgenic cell line with the piggyBac™ system...However, several challenges still remain, such as making gene edits completely seamless (i.e., so no other...piggyBac™ transposase (PBx), which contains mutations making it competent for excision, but defective for integration...larger piggyBac™ donor vector, it is possible to make the ITR and selection cassette disrupt the target...

... to read it, give it back to you, and for you to make the relevant alterations before submitting - handing...otherwise why would you be interested in it?) so make sure that comes across. Check out some in-depth ...agencies want value for their money so you need to make it clear that you will be doing your research in...routinely isn’t common to non-scientists and may make your perfectly reasonable paragraph unintelligible... about what I hope to discover. This section may make the difference in whether you get funding or not...Improve Your Science Communication Skills 5. Practice makes perfect, apply for small grants too There are actually... awarded money to carry out research, be sure to make it clear what the outcome of the work was. Ideally...

... As a member of the Quality Control team, I help make sure the plasmids that go out our doors are correct...assistant in a lab at the university that I went to. Making that switch from being a grad student to an employee...she didn’t want to take it. “I realized if I don’t make it to med school it’s not the end of my life, even...programs. I thought, ‘Let me pursue this, let me make a slight change so I can have flexibility and be...is incredibly helpful to me to know that you can make big changes in your career path and still find comfort...never work a day in her life. She said, “it now makes sense because I love working in the lab and being...people and for the people, feeding the people, and making them get outside of their comfort zones. I came...

...to Ireland and take a chance as entrepreneurs. To make things more interesting, we are working in a new.... Being unable to publish or attend conferences makes it very challenging to compete in today’s tough ... a lot of time talking about what we could do to make ourselves marketable to the wider world and occasionally...Keira’s an optimist, I’m a realist, and together we make a pretty good team. We have confidence that we can...the lines of “I hate GMOs, but what you are doing makes sense. I can support that.” That was interesting...technology we will need, and the business training to make the most of it. It has also allowed us to really... we enjoy and see a future in. And we decided to make education and outreach a cornerstone of our business...

...this make things more manageable, but it also keeps stock solutions from getting contaminated. Make sure...minutes before you start any tissue culture work and make sure that clean air is flowing in. The vent for ...not be used as storage cabinets. Clutter not only makes it difficult to clean your workspace properly, but...disrupt the laminar air flow around your work area. Make sure to only bring required materials into the hood...stay out too long While you’re preparing things, make sure your cells don’t stay out too long. Cells usually...indicates that the cells have grown enough, are making their surrounding media acidic through the secretion...all but one in liquid nitrogen. The frozen vials make up your master stock, while you can use the remaining...

...more easily identify what to delegate and to whom (make sure that person has time for it!). Then schedule...not a clear deadline. Finally check-in regularly, make yourself available, and encourage questions. Unless...opportunities. I still need to check myself sometimes to make sure I am delegating well, but it does get easier...grateful for the feedback, but at least you can start making changes for the best. Positive feedback is equally...experienced to guide you - is valuable. Protect your team Make you team feel safe. Your team members need to trust...to encourage them to speak their minds. They will make mistakes and you will take responsibility (within... and help you grow in your role. The latter will make you feel discouraged and not valued which will undoubtedly...

...you use depends on your preferences. Either way, make sure to test your colony PCR primers before using...insert or if the insert is in your plasmid Have to make a new primer pair for each insert Backbone-Specific...plasmid Great for blunt end cloning You have to make a new primer pair for each insert since primers ...positive ones. Regardless of which method you choose, make sure to use the appropriate antibiotic for selection...polymerase is sufficient. Controls: Controls can make or break an experiment. The best controls for a ...products on an agarose gel to determine their size. Make sure to use an appropriate molecular weight standard...doesn’t mean there aren’t mutations in your insert. Make sure to submit multiple positive clones for sequencing...

...manager quickly make the connection between your skills and the needs of the job. Make it clear that you...successful, reproducible assay. That information can make you a much stronger candidate!  Additional uses ...cover letter to explain why your transferable skills make you a good candidate for the position. You can also...could talk about how your experience with qPCR would make it easy to master ddPCR.  Finally, it’s a good idea...highlight what specific skills, qualities, or interests make you a good candidate for the job. The body of the...should be one to three paragraphs. This is where you make the case for why you are a good candidate, explain...start from a standard version, but take some time to make it fit the job you’re applying for. The cover letter...

...philosophy Customer service can be a hassle. You finally make it through a 20-step phone tree, only to be left... customer support with self service We strive to make our support team available whenever you need them...not have the chance to stop what you’re doing and make a phone call or write out an email. You need help...resources, we will continually update the Help Center to make sure that, whatever your support needs are, we’ve...ll navigate to articles answering your questions making it easier than ever to get the support you need... regular site, www.addgene.org. We’re excited to make these new features available to the Addgene community...valuable feedback. This is Addgene after all, and making your research easier always comes first. Resources...

...produced in abundance, making them useful for in vivo studies. These attributes make frog eggs some of the...physiological, or developmental characteristics that make them ideal for studying in a laboratory environment...Additionally, its status as a Biosafety Level 1 microbe makes it a perfect candidate for introductory teaching...Iannaccone et al., 2009). Rats are larger than mice making them more useful for certain studies that involve...axolotl has several attractive characteristics that make them an ideal model organism; they are relatively...genetics and developmental patterns are conserved, making them great models for human disease research. One... alike.  Arabidopsis has many of the traits that make an ideal model organism: quick development, a large...

...’s critical to make these decisions in the following order of importance: 1. First make decisions based... Then make decisions based on financial values. All too often, we see early investigators making unnecessary...step by step instructions on creating a budget, making decisions based on that budget, and negotiating...science will define your success. Therefore, it only makes sense to reverse engineer your budgetary needs from...you can eliminate from your budget. Every cut you make signifies that you've made a conscious effort to...dependent on a pathology microtome/cryostat, then making a careful decision on the condition and availability...

... studies, making a meta analysis available to the community on ReFigure. ReFigure also makes it easier...during this period occurs infrequently and rarely makes it into the small selection of data found in full-length...early career researchers, we wondered how we could make the insights gained during this time more visible... on science. ReFigure saves time, knowledge and makes your insights discoverable. Watch this quick video...cell lines/model systems ReFigure allows you to make meaningful collections from a large section of open...paper they’re reading has been linked in a ReFigure making it easy for others to discover your insights on...

...culture towards a more generous and rewarding way of making science.   How we started Crowdfight-COVID19...Confirm the expert’s suitability and availability; 3) Make the contact to start a direct collaboration between...realization that there is a great untapped potential to make science more efficient, open, and collaborative....collaborative. We now want to use this potential to make a difference not just against COVID-19, but also in other...These are transient partnerships where a researcher makes a small but crucial contribution to someone else...We are a community of researchers who believe in making science less of an individual effort and more of...bono. If this sounds appealing, join us and help make science a better place. You will receive opportunities...

...summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you...with the cells’ endogenous ubiquitin ligase, the Kanemaki lab was able to translate AID to yeast, as well...Nishimura et al., 2009, Natsume et al., 2016).  The Kanemaki lab previously developed a CRISPR/Cas9 based method...essential proteins (Natsume et al., 2016). Now, the Kanemaki lab has created a new toolkit of plasmids for ...Yesbolatova et al., 2019) using CRISPR. This toolkit makes it easy to tag your protein of interest to create...create conditional human cell lines. Lastly, the Kanemaki lab demonstrated that auxinole, a TIR1 inhibitor...such as a distal promoter and an enhancer. What makes LADL unique from previous iterations is the addition...

...Alzheimer's - a connection I wouldn’t have thought to make (even if science writer Ed Yong’s book “I Contain... skills you’ve developed in your current work to make new insights in neuroscience. Even if you're currently...expertise and can be difficult for individual labs to make efficiently. For select viral vectors in the repository...repository, you can instead have Addgene make the virus for you. Neuroscientists are particularly interested...experiments. This helped reinforce our goals of making more AAVs and educational content to help researchers...for future experiments and to find tools that will make your transition into the field much easier! If there... suggest a plasmid to us, request that we start making the AAV, or help us produce new educational content...

...rushing, you’re much more likely to make a mistake. When you make a mistake, you’re more likely to be ...and the same goes for your workflow. Next time you make a mistake, instead of silently vowing never to do... physical copy of the protocol when you work and make a checkmark next to each step as you complete it...experimental steps are worth doing, and doing well, so make sure you’re taking time to do them carefully and...reach for spreadsheets, printouts, and even label makers to keep things legible. It is faster to spend more...however, many ways for you to reduce the chances of making an error. Try a variety of things, including (and...

...as a great first-time mentoring experience. They make mentoring less mysterious and train the participants...Provides a good mechanism for accountability that makes mentoring effective Goal setting is easier with ...example, a group of people who are all interested in making a transition. Schedule your first meeting and get...regularly no matter what, even if one person can’t make it. One of the biggest group mentoring predictors...breakfast once a month. All participants must commit to making the meetings a scheduling priority (and last minute... Set concrete goals with input from the group to make the plan better. Track your goal progress as a group...the series to get more advice on mentoring: What Makes a Good Mentor? Choosing a Good Mentor for Scientists...

...during your current job and note what makes your heart sing, what makes you pull your hair out, your successes...opportunities to pivot and grow along the way. That’s what makes it so wonderful. The most challenging part of jumping... in a government setting: drafting legislation, making rules, and implementing the policy within the jurisdiction...career or a part-time passion, there are many ways to make it work for your time and interests.  Action Items... healthcare systems so I could understand how to make new treatments and technologies accessible to patients...healthcare system? Defining your why is what will make you a competitive candidate for any science policy...your interest to future positions, but will also make your transition from academia to the policy world...

...of the Cas9 nuclease (PDB ID: 4OO8 and 4UN3), Slaymaker et al. hypothesized that Cas9 cutting efficiency...enough to keep off-target sites from being cut. Slaymaker et al reasoned that, if they decreased the positive...the electropositivity of the HNH/RuvC groove, Slaymaker et al. made a variety of alanine substitutions...off-target effects genome-wide. Further work by Slaymaker et al. showed that these mutants are not toxic...SpCas9-HF1, and HypaCas9 should enable researchers to make precise edits in mammalian cells and may decrease...look forward to seeing how the research community makes use of these tools and are excited to see continued...improvements in genome engineering! References 1. Slaymaker, Ian M., et al. "Rationally engineered Cas9 nucleases...