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We narrowed to 515 results for: gan

Showing: 41 - 60 of 515 results

  1. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    Published
    May 18, 2018, 7:32 p.m.
    ... with the Harvard GSAS Science Policy Group to organize a Minisymposium on Reproducibility. The minisymposium... additional resources and information about organizations promoting reproducibility in this booklet (which...within a published manuscript. Luckily, many organizations have been working to provide tools that make...expand to other protein technologies later. Organizations such as BenchSci and FPbase are working hard... they’ve used for data analysis while other organizations like Dryad make it easier for researchers to...are blogs - both hosted by individuals and by organizations like Addgene (a good example is our series of...editing technology NgAgo). In addition, there are organizations like ReFigure which aims to consolidate published...

  2. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    Published
    Sept. 28, 2020, noon
    ...has taken place in other organisms. In many bacteria, unlike other organisms, CRISPR-induced double stranded...diseases have connections to either specific organisms or organism/gene interactions with the host. This scenario...can consider the microbiome as a collection of organisms, their genes, and metabolic processes, occupying...to diseases where the presence of just a single organism, such as Clostridium difficile, could be targeted... This approach successfully targeted specific organisms when using bacteriophages or mobile plasmids in...Pseudomonas species Expanding this tool to other organisms, the Ji lab brought genome and base editing to...genome engineering remains challenging for most organisms in the genus. Work from the Sun lab demonstrates...

  3. Reproducibility for Everyone: Lessons from an Open Science Collaboration

    Type
    Blog Post
    Published
    Feb. 28, 2019, 1:34 p.m.
    ...In 2017, Lenny Teytelman, CEO of protocols.io, organized a panel to discuss reproducibility issues in research... there were other academics and like-minded organizations with similar ideas who were also thinking about... an eLife ambassador, had previous experience organizing open access events and grassroots efforts. After...in developing reproducibility workshops and organizational skills. Meanwhile, Addgene was also thinking... in the year and in the fall of 2018, I helped organize a workshop for community scientists at the Global... would want to know before, during, and after organizing such a workshop. Finally, the group put a lot...IS-MPMI. If you’re interested in having a workshop organized at your upcoming conference or you’d like to join...

  4. Educational Planarian Tools for Teaching Developmental Biology Techniques

    Type
    Blog Post
    Published
    May 24, 2018, 1:20 p.m.
    ...Girardia sp., Dugesia dorotocephala, Phagocata morgani, and Phagocata gracillis. There are 11 different...developmental biologists have to extract RNA from their organism of interest, use the RNA to create a complementary... in  the labeling specific cell types or entire organs in planarians. For example, the gene Piwi labels...discovered and characterized in an unrelated worm (C. elegans) [2]. Delivering dsRNA matching a specific mRNA...strain that is designated as DE3. For Phagocata morgani and Phagocata gracillis, the process is a little...E. coli. Thus, to get the dsRNA into Phagocata morgani and Phagocata gracillis, one has to purify the ...interference by double-stranded RNA in Caenorhabditis elegans. Nature, 391 (1998), pp. 806-811. PubMed PMID: ...

  5. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    Published
    July 23, 2015, 2:30 p.m.
    ...models of C. elegans molecular biology are based on the standard worm genome. While C. elegans are often ...of active spaces near you.   Learning about C. elegans with the OpenWorm Project One such space is the...project. The main goal of OpenWorm is to simulate C. elegans as a computer model. When I first heard about it...often thought of as simple organisms , they have many levels of complexity, especially when trying to explore...and understand this complexity using existing C. elegans mutants. For instance, we use a mutant known as...collection of samples and sequencing is being organized and funded by someone else (KittyBiome has been...

  6. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    Published
    Feb. 25, 2016, 3:30 p.m.
    ...educating the public and organizing events. I contacted local professional organizations and nonprofits. I volunteered...Central Indiana Science Outreach, or CINSO. We organize fun science events for adults and professional... test and discover what career suits you best I began viewing my activities as types of experiments. I...communication, editing, marketing, and website organization. My short experience with my blog helped immensely...establishments such as museums, professional organizations, and other nonprofits to gain support for CINSO...graduate experience, the point at which my career path began to take shape, was when I started volunteering. ...

  7. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    Published
    Sept. 3, 2015, 2:30 p.m.
    ... priority and have organized many events over the years. To keep the organizational side of things from...Use it! 8. Organize If you’re doing an event that requires people to be in groups, organize your troops...event! Addgene company culture At Addgene we organically developed the philosophy of  “work hard, play... in getting the fun started. I’m aware that  “organizing the fun” sounds a little neurotic, but hey- sometimes... her operational responsibilities, she enjoys organizing team building events at Addgene and cultivating...

  8. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    Published
    May 21, 2024, 1:30 p.m.
    ...asks for strong organizational skills. You might think, “I’d be good at that! I organized my lab data so...important part of the application. Even within an organization, there can be variance. At Addgene, our content...explain why you are interested in working for that organization or group. This is particularly important for...want to talk about! You could describe your organizational system, your rationale for designing it the...title of the position] at [name of the company/organization]. I am a [adjective that describes you] scientist...that particular job, your commitment to the organization's mission, or your deep interest in the lab's...

  9. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type
    Blog Post
    Published
    Feb. 18, 2020, 2:15 p.m.
    ...Williams, Dave Baltrus, Geof Hannigan, Irene Newton, Juliet Johnston, Kat Milligan-Myhre, Kim Dill-McFarland...discussion with my dinner partners Denise Akob and Geof Hannigan that the outline for Binning Singletons came together...a positive stir and was recognized by meeting organizers in their town hall.  Iterative improvement - ...Mentor-Singleton relationship. Some of the variability was organic, as some people need less assistance than others...take some leaps. Binning Singletons is its own organization, working to make scientific meetings more productive...Eric Griffis, Janet Iwasa, Shawnna Buttery, Kat Milligan-Myhre, and Pragati Chengappa.  Financial and moral...

  10. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...orphan receptors meaning their ligands are unknown. Identifying new ligands for orphan GPCRs is of significant...Caenorhabditis elegans CRISPR/Cas9 technology has revolutionized genome editing in multiple organisms thanks ...construction of a so-called “genomically recoded organism” (3) but its portability to other bacterial strains...monitoring and analysing autophagy of cytosol and organelles in yeast cells. Named after the brightly-coloured... both in vitro cell systems and diverse model organisms. Addgene’s newest TALEN kit, the FusX assembly...screens required to identify correctly modified organisms has limited routine use of this technology. To...CRISPR/Cas9-based genetic tag insertion in the C. elegans genome, Erik Jorgensen’s lab from the University...

  11. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    Published
    March 5, 2019, 1:08 p.m.
    ... (XFP) of different colors to label an organ or entire organism. Because each cell can have multiple copies...The ability to track single cell dynamics at the organism level has been made possible by tools that allow.... Genes encoding the fluorescent proteins are organized in tandem with a single promoter, but only the...developed for tracking various cell types and organisms and for improving the level of detail possible...that allow for multicolor labeling of an entire organism or a specific tissue. XFPs are introduced using...systems allow single cell dynamics in a variety of organisms to be observed in more detail than ever before...

  12. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ...an essential coenzyme that is required by all organisms for life. Biotin is only synthesized by plants...enzymes catalyze the transfer of carboxyl groups to organic acids to produce important cellular metabolites...post-translational protein modification. Most organisms have less than five enzymes in the entire proteome...for biotinylating only one lysine in the entire organism on the biotin carboxyl carrier protein (BCCP)....them is one of the strongest noncovalent protein-ligand interactions found in nature. Once the bond is ...is unaffected by changes in pH, temperature or organic solvents. Uses of biotin in molecular biology Biotin...these features it isn’t surprising that scientists began to exploit biotin and its strong interaction with...

  13. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...tethered ligands hold the ligand in close proximity to the receptor, when exposed to light, the ligand engages...photoswitchable tethered ligands (PTL), and photoswitchable, orthogonal, remotely-tethered ligands (PORTLs). OptoXRs... them. PORTLs use a similar concept, except the ligand is attached to a protein binding partner or nanobody..., McKnight, D. J., Yoshizawa, S., Kato, H. E., Ganguli, S., & Deisseroth, K. (2019). Cortical layer–specific...

  14. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    Published
    Dec. 14, 2020, 2:15 p.m.
    ...transducer complexes that are activated in response to a ligand binding a GPCR of interest. How does TRUPATH work...intracellular surface of the GPCR. Binding of a ligand to the extracellular surface of the GPCR causes...coelenterazine, followed by the addition of the endogenous ligand or drug. In the inactive state, the luminescent...emission. Following activation in response to the ligand, the complex will disassociate, resulting in a ...across the transducerome for a specific receptor-ligand pair. They further used TRUPATH to analyze a panel...This collection can be used to study endogenous ligands, the pathways they activate, as well as the activation...

  15. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    Published
    June 11, 2020, 1:34 p.m.
    ...designer ligands that can be simply introduced by injection. The main challenge in using ligands is ensuring...at broad tissue or system-wide targeting, as the ligand can reach target cells throughout the body. Controlling...chemogenetics, while different concentrations of ligands can be injected, this is not as precise and the...brain. One way to address this is by working with organisms with light accessible brains, such as zebrafish...while also being selective and effective. A new ligand from the Bryan Roth lab and Takafumi Minamimoto...

  16. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    Published
    June 5, 2017, 3:19 p.m.
    ...nonprofit" organization what do you think of? Maybe the term brings to mind a social service organization like... about a local food pantry or community arts organization. Many people are surprised to learn that Addgene...nonprofits do with their revenue. A nonprofit organization cannot distribute profits to private individuals...as a nonprofit, we attract partnerships with organizations that share our community-oriented values. Addgenies...Meron, Michael Lo., Michael Le., Michel, Michelle, Morgan, Natashia, Nicole, Olivia, Pamela, Racheal, Ramsey...

  17. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    Published
    Jan. 25, 2018, 2:09 p.m.
    ...affinity for the ligand, especially if the modified amines are located near the ligand binding site. A ...unbound material to calculate the amount of bound ligand (after subtracting out non-specific binding). Historically...dimers, may influence binding kinetics of the target ligand. Another important consideration is whether the...the affinity of an experimental protein-ligand interaction approaches that of GST-glutathione. Biotin-...measurement of high affinity (i.e. sub-nanomolar) protein-ligand interactions. Avidin and its derivatives have four...

  18. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ...serve as slaves.  Meganucleases In 1989 the first ever DNA-editing enzymes, meganucleases, were discovered...capability of transforming the genetic code of living organisms and its societal implications even before the ...pneumoniae bacteria could be transformed from one organism to the other. This is now famed as the Griffith...genetics and SciFi.  Scientists around the world began looking for the magic molecule that serves as the...in 1990, just a year after the discovery of meganucleases. The storyline involves the resurrection of ...of all the endonuclease-based gene editors (meganucleases, ZFNs, TALENs, and CRISPR-Cas) is that they ...

  19. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    Published
    Oct. 27, 2020, 1:15 p.m.
    ...many organisms. When supplemented with adenine analogs such as 2-fluoroadenine (2-FA), organisms with ...Zurich. He is fascinated by how organisms exquisitely and robustly self-organize at the cellular and multicellular...Fortunately, researchers studying plants and other organisms lacking this innate immunity need not worry and...plants Once you’ve introduced your RNAi into your organism, how do you identify the actively silenced ones... of APT and any gene target of interest, only organisms that are actively silencing to a sufficient degree...

  20. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    Published
    June 23, 2022, 1:15 p.m.
    ... post was originally written in 2014 by Kendall Morgan and updated in 2022 by Lucie Wilson. Lucie is an...used to generate mutations in a wide range of organisms, but its potential to efficiently correct disease...functional correction of the gene in clonally expanded organoids. “CRISPR in adult stem cells has huge potential...potential, because the genomes of adult stem cells in organoid cultures are stable,” said Gerald Schwank, first..., however, because the disease affects multiple organ systems. Nevertheless, the findings are proof-of-principle...of CFTR by CRISPR/Cas9 in Intestinal Stem Cell Organoids of Cystic Fibrosis Patients." Cell Stem Cell. ...
Showing: 41 - 60 of 515 results