CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More
Type
Blog Post
Published
Sept. 28, 2020, noon
...has taken place in other organisms. In many bacteria, unlike other organisms, CRISPR-induced double stranded...diseases have connections to either specific organisms or organism/gene interactions with the host.
This scenario...can consider the microbiome as a collection of organisms, their genes, and metabolic processes, occupying...to diseases where the presence of just a single organism, such as Clostridium difficile, could be targeted... This approach successfully targeted specific organisms when using bacteriophages or mobile plasmids in...Pseudomonas species
Expanding this tool to other organisms, the Ji lab brought genome and base editing to...genome engineering remains challenging for most organisms in the genus. Work from the Sun lab demonstrates...