...two-part series, we continue our conversation with Niroshi Senaratne and Ben Vincent from the Biological and...environment 3:09 - 5:58 : Specific Examples where Niroshi and Ben helped build their grad student communities... discussion, we bring up a performance Ben and Niroshi organized for Tyler's (the host's) thesis defense...students discuss the third year dip (see graph). Niroshi and Ben give their insights as to what causes this... made to the grad school process 22:41 - END : Niroshi and Ben's favorite advice to give to younger graduate...and Plasticity in Mentoring" We'd like to thank Niroshi and Ben for taking the time to sit down and talk...Systems Biology, and he tweets @benjvincent.     Niroshi Senaratne is a graduate of the PhD program in Genetics...

...and the pH shift can be prevented. Keep this information in mind if you’re planning on shipping something...We’re always looking for ways to improve our shipment processes. After reading a publication describing...describing how short term storage on dry ice can shift sample pH, we wondered whether or not the dry ice we ...we use to keep viruses frozen during shipment was having an impact on the samples. We therefore devised...for 48 hours (to replicate how our viruses are shipped), where they would be exposed to a closed environment...function optimally at -80°C. For samples that are shipped on dry ice, this can affect the pH of the sample...

...expertise in shipping hundreds of plasmids a day, the Lab and Office Teams develop shipping systems and...accelerating research and discovery, while also pushing us to challenge assumptions, holding us to nonprofit... Addgene’s high quality standards?” Under the leadership of Addgene’s co-founder, Melina Fan, we created...viral samples. The Office Team made sure we could ship internationally and be ready to provide extensive...extensive logistics support for export and shipping on dry ice. The Business and Finance Teams continue to facilitate... facilitate the formation of partnerships to expand the service and figure out what we can afford to do...

...to be shifted away from an axis, decrease the compensation value. If neither of these helps shift your ...Figure 1). In Figure 1A, the population needs to be shifted towards the x-axis, so you would increase the compensation... value for APC. If the population needed to be shifted away from the x-axis, you would decrease the compensation...determine which way your population needs to be shifted, consider your perpendicular lines. In this case...so our circled population on the right needs to shift towards the X axis to become a perpendicular vertical...control samples: if the population needs to be shifted towards an axis, increase the compensation value...

... more open to developing a relationship. The time to develop relationships is before you need them (well...doing interesting things. I call this building relationships because networking has gotten a bad reputation...Scientists enjoy learning new things. Building new relationships is all about learning new things from other ...networking for a job connection with ongoing relationship building through Informational Interviews. Making...is always best to meet in person to develop relationships, but if it is not possible consider a phone ...

... of the time because of relationships. It’s much harder to build relationships when you are more desperate...Here we are seven months past a worldwide shift to work-from-home and just now realizing that we can’...throughout our careers. An easy way to access great mentorship is by recruiting your own peers and classmates...experiences to share. The conversation builds a relationship for the future and the 1:1 format is good for...you will make good progress on both building relationships and thinking about future career options. 5....

...next month?” Some mentoring relationships grow naturally out of friendships or work interactions. Watch...watch my webinar, “Not Networking 101-Building Relationships for Success”. Choose 1-2 tactics to try. One...be a bit random because you never know which relationships will take off, or who your new contact will ...current organization but also important to build relationships outside your organization. The mentor in your...other ways they can help you grow. Mentoring relationships can last for an hour or for a year. Some click...

...Cancer Institute (NCI) has an internship similar to Susan’s NEI internship. Application information can...a master’s, Susan completed a science writing internship with the National Eye Institute (NEI), where ...audience. She then got a full time position with a Washington D.C.-based communications consulting firm, Palladian...Communications and Public Relations at the University of Washington and the connections she made through this program...started her scicomm career: “ I think living in Washington D.C. helped because there was so much work through...

... despite the evolving export/import rules. Our shipping and lab teams continue to keep up with the demand... for more offerings. The viral vector team is publishing research, hosting conferences, and are expanding...have confidence that from Business Development to Shipping we will execute any new projects with our usual...much potential for service and contribution! Leadership On an organizational level, we keep passing...vigilant about resources, I’m not worried about the stewardship of this in the hands of our finance team, now...often focus on the excitement of what we are accomplishing and what more we might do. I am grateful every...

...like-minded science communicators? Attend the annual flagship meetings of AAAS, WCSJ or ECSJ. These meeting ...in the UK, join the SciComm mailing list. 7) Internships – Learn on the job Nothing prepares you better...better than learning on the job. Try to get an internship at a magazine or newspaper (here’s a partial list...). A great opportunity is the AAAS Mass Media Fellowship, which places science students for 10 busy weeks...American or National Geographic. Similarly, media fellowships from the British Science Association allow you...Society of Experimental Biology offers press internships to cover their annual meetings. 8) Training –...

...https://doi.org/10.1126/science.aat0839 Yoshimi, K., & Mashimo, T. (2022). Genome editing technology and...all-in-one Cascade plasmids — such as the Tomoji Mashimo lab and the Tilmann Weber lab — to increase efficiency...doi.org/10.1016/j.molcel.2023.01.024 Morisaka, H., Yoshimi, K., Okuzaki, Y., Gee, P., Kunihiro, Y., Sonpho...Yamamoto, T., Sano, S., Hotta, A., Takeda, J., & Mashimo, T. (2019). CRISPR-Cas3 induces broad and unidirectional...s41587-019-0310-0 Makarova, K. S., Wolf, Y. I., Alkhnbashi, O. S., Costa, F., Shah, S. A., Saunders, S. ...

...skills that create a full package and add an extra shine to your work. Knowing your transferrable skills ...using your charisma to give people hope for some shiny vision on the horizon. You’re about to keep silent...opportunity much different? Might you already have leadership skills that are just not being tapped at the ...this time to step up and develop your nascent leadership skills. You might just find that you’re more ...didn't even know you possessed. Think about your shining moments. Recall a few times you really felt great...Managing a team Conflict resolution Organization Leadership Problem Solving Creativity Data analysis Listening...

...contributed by Ryan Watkins, Professor at George Washington University and developer of wesharescience.org...English is a second or third language (making publishing their research all the more challenging even ...Hamilton, biology professors at the University of Washington in St. Louis, offer guidance on how to make a...Rhythm and Melatonin for the Timing of Nocturnal Courtship Vocalization. The video summarizes the research...science! Ryan Watkins is a professor at George Washington University and he the developer of the WeShareScience.org...journals: An overview and case study." Journal of Librarianship and Scholarly Communication 2.2 (2014): 2. Additional...

...pCAG-EGxxFP target plasmid and pX330-sgRNA plasmid (Mashiko et al. 2013). The pCAG-EGxxFP target plasmid contains...indicates a more successful gRNA design. Adapted from Mashiko et al., 2014. Not all gRNA’s are effective....fluorescent signal, for details on this plasmid, see Mashiko et al., 2013). Testing the efficacy of your gRNAs...validation assay (above) and it is just as effective (Mashiko et al., 2014). The plasmid is sturdier than the...24253446. PubMed Central PMCID: PMC3875854. 2. Mashiko, Daisuke, et al. "Generation of mutant mice by ...24284873. PubMed Central PMCID: PMC3842082. 3. Mashiko, Daisuke, et al. "Feasibility for a large scale...

...formal stance taken by Addgene or its staff. In Washington, D.C. as well as sister locations on April 22...inherently political so why is there a march in Washington, D.C., the seat of the United States government...citizens. Thus civilization proceeds in an informed fashion. For science to be useful to governments, however... for Science has the details for the march in Washington as well as the satellite marches. Anyone can ...Climate Change References Purged from Website The Washington Post: Trump administration tells EPA to freeze...Line The New York Times: A Scientists’ March on Washington Is a Bad Idea Pew Research Center: Public Praises...

...facilitating connections to mentorship and networking opportunities, publishing assistance, and more to Instrumental...everywhere, but resources are not. Through its flagship program, Instrumental Access, Seeding Labs partners...research in the labs they’re granted to, as well as shine a light on some of the great minds working in developing...Castano Corsino’s Recent Post on the International Mentorship Program Learn How to Network and Meet People ...

...Cas9 compared to ZFNs and TALENs,” said Tetsushi Sakuma of Hiroshima University. “However, there had been...now available thanks to researchers at Japan’s Hiroshima University who described their new tool in Scientific...they’ve filled that gap. As Sakuma and colleague Takashi Yamamoto describe in their recent study, the construction...all-in-one CRISPR/Cas9 vector system. Sakuma T, Nishikawa A, Kume S, Chayama K, Yamamoto T. Sci Rep. 2014...

...’t until much later when, in the 1960s, Osamu Shimomura studied the bioluminescent properties observed...green light. To investigate this green glimmer, Shimomura collected many, many jellyfish specimens from ...Sound in the Pacific Ocean, off of the coast of Washington state. Using these samples, he was able to isolate...mutations that alter and improve its function and shift its emission spectrum. New fluorescent proteins ...when the Nobel Prize in chemistry was awarded to Shimomura, Chalfie, and Tsien for their work on GFP. This...

...speakers from academia, industry, nonprofits, and publishing. The livestream video from the event can be found...should be reproducible. Indeed, researchers are pushing the limits of knowledge as they develop new ways...single, searchable space. Finally there are new publishing outlets like F1000, PeerJ, PLoS, and bioRxiv ...adoption of policies that promote reproducible publishing practices. One example is the publication of ...Within universities, we’ll also hopefully see a shift toward hiring and promotion practices that focus... discussion to learn a bit more about how the publishing community views reproducibility, how industry...

...conferences. The Barry Goldwater scholarship is also a national scholarship that supports students interested...t know yet has helped me cultivate stronger relationships with my labmates. Sometimes what I thought was...makes all the difference in a lab experience. “Mentorship” was a bit of a foreign concept to me when I ...online for a while and observed. Apply for those scholarships that you don’t think you’re qualified for. Wayne...populations. Research universities often have scholarships and funds dedicated for undergraduates interested...